Journal of Endodontics
Volume 31, Issue 8 , Pages 605-607, August 2005

Biocompatibility In Vitro Tests of Mineral Trioxide Aggregate and Regular and White Portland Cements

  • Daniel Araki Ribeiro, PhD

      Affiliations

    • From the Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu Medical School—UNESP
    • Corresponding Author InformationAddress request for reprints to Dr. Daniel Araki Ribeiro, PhD, TOXICAN, Departamento de Patologia, Faculdade de Medicina de Botucatu—UNESP, Distrito de Rubião Jr s/n Botucatu, SP Brazil 18618-000
    • ,
  • Marco Antonio Hungaro Duarte, PhD

      Affiliations

    • Department of Dental Clinics, University of Sagrado Coração—USC.
    • ,
  • Mariza Akemi Matsumoto, PhD

      Affiliations

    • Department of Dental Clinics, University of Sagrado Coração—USC.
    • ,
  • Mariangela Esther Alencar Marques, PhD

      Affiliations

    • From the Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu Medical School—UNESP
    • ,
  • Daisy Maria Favero Salvadori, PhD

      Affiliations

    • From the Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu Medical School—UNESP

Abstract 

Mineral trioxide aggregate (MTA) and Portland cement are being used in dentistry as root end-filling materials. However, biocompatibility data concerning genotoxicity and cytotoxicity are needed for complete risk assessment of these compounds. In the present study, genotoxic and cytotoxic effects of MTA and Portland cements were evaluated in vitro using the alkaline single cell gel (comet) assay and trypan blue exclusion test, respectively, on mouse lymphoma cells. The results demonstrated that the single cell gel (comet) assay failed to detect DNA damage after a treatment of cells by MTA and Portland cements for concentrations up to 1000 μg/ml. Similarly, results showed that none of the compounds tested were cytotoxic. Taken together, these results seem to indicate that MTA and Portland cements are not genotoxins and do not induce cellular death.

Key Words:  Mineral trioxide aggregate , Portland cement , comet assay , genotoxicity

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PII: S0099-2399(05)60868-0

doi:10.1097/01.don.0000153842.06657.e2

Journal of Endodontics
Volume 31, Issue 8 , Pages 605-607, August 2005

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