Journal of Endodontics
Volume 33, Issue 6 , Pages 667-672 , June 2007

Bacterial Reduction in Infected Root Canals Treated With 2.5% NaOCl as an Irrigant and Calcium Hydroxide/Camphorated Paramonochlorophenol Paste as an Intracanal Dressing

  • José F. Siqueira Jr, DDS, MSc, PhD

      Affiliations

    • Corresponding Author InformationAddress requests for reprints to Dr. José F. Siqueira Jr, Faculty of Dentistry, Estácio de Sá University, Av. Paulo de Frontin 628, 5o andar, Rio de Janeiro, RJ, Brazil 20261-243.
  • ,
  • Karen M. Magalhães, DDS
  • ,
  • Isabela N. Rôças, DDS, MSc, PhD

References 

  1. Sjögren U, Hagglund B, Sundqvist G, Wing K. Factors affecting the long-term results of endodontic treatment. J Endod. 1990;16:498–504
  2. Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997;30:297–306
  3. Engström B, Hard AF, Segerstad L, Ramström G, Frostell G. Correlation of positive cultures with the prognosis for root canal treatment. Odontol Revy. 1964;15:257–270
  4. Waltimo T, Trope M, Haapasalo M, Orstavik D. Clinical efficacy of treatment procedures in endodontic infection control and one year follow-up of periapical healing. J Endod. 2005;31:863–866
  5. Fabricius L, Dahlén G, Sundqvist G, Happonen RP, Möller AJR. Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci. 2006;114:278–285
  6. McGurkin-Smith R, Trope M, Caplan D, Sigurdsson A. Reduction of intracanal bacteria using GT rotary instrumentation, 5.25% NaOCl, EDTA, and Ca(OH)2. J Endod. 2005;31:359–363
  7. Byström A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985;18:35–40
  8. Shuping GB, Orstavik D, Sigurdsson A, Trope M. Reduction of intracanal bacteria using nickel-titanium rotary instrumentation and various medications. J Endod. 2000;26:751–755
  9. Sjögren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J. 1991;24:119–125
  10. Peters LB, van Winkelhoff AJ, Buijs JF, Wesselink PR. Effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. Int Endod J. 2002;35:13–21
  11. Byström A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol. 1985;1:170–175
  12. Portenier I, Haapasalo H, Rye A, Waltimo T, Orstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J. 2001;34:184–188
  13. Waltimo TM, Siren EK, Orstavik D, Haapasalo MP. Susceptibility of oral Candida species to calcium hydroxide in vitro. Int Endod J. 1999;32:94–98
  14. Siqueira JF, de Uzeda M. Disinfection by calcium hydroxide pastes of dentinal tubules infected with two obligate and one facultative anaerobic bacteria. J Endod. 1996;22:674–676
  15. Siqueira JF, de Uzeda M. Influence of different vehicles on the antibacterial effects of calcium hydroxide. J Endod. 1998;24:663–665
  16. Menezes MM, Valera MC, Jorge AO, Koga-Ito CY, Camargo CH, Mancini MN. In vitro evaluation of the effectiveness of irrigants and intracanal medicaments on microorganisms within root canals. Int Endod J. 2004;37:311–319
  17. Sukawat C, Srisuwan T. A comparison of the antimicrobial efficacy of three calcium hydroxide formulations on human dentin infected with Enterococcus faecalis.. J Endod. 2002;28:102–104
  18. Gomes BP, Ferraz CC, Garrido FD, et al. Microbial susceptibility to calcium hydroxide pastes and their vehicles. J Endod. 2002;28:758–761
  19. Siqueira JF, Rôças IN. Exploiting molecular methods to explore endodontic infections: Part 1—current molecular technologies for microbiological diagnosis. J Endod. 2005;31:411–423
  20. Chu FC, Leung WK, Tsang PC, Chow TW, Samaranayake LP. Identification of cultivable microorganisms from root canals with apical periodontitis following two-visit endodontic treatment with antibiotics/steroid or calcium hydroxide dressings. J Endod. 2006;32:17–23
  21. Clarridge JE. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev. 2004;17:840–862
  22. Siqueira JF, Rôças IN, Favieri A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod. 2000;26:331–334
  23. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–410
  24. Munson MA, Pitt-Ford T, Chong B, Weightman A, Wade WG. Molecular and cultural analysis of the microflora associated with endodontic infections. J Dent Res. 2002;81:761–766
  25. Sakamoto M, Rôças IN, Siqueira JF, Benno Y. Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol. 2006;21:112–122
  26. Siqueira JF Jr, Guimarães-Pinto T, Rôças IN. Effects of chemomechanical preparation using 2.5% NaOCl and intracanal medication with calcium hydroxide on cultivable bacteria in infected root canals. J Endod (in press).
  27. Sakamoto M, Siqueira JF, Rôças IN, Benno Y. Bacterial reduction and persistence after endodontic treatment procedures. Oral Microbiol Immunol. 2007;22:19–23
  28. Chávez de Paz LE. On bacteria persisting root canal treatment. Identification and potential mechanisms of resistance to antimicrobial measures [PhD thesis]. Göteborg, Sweden: Göteborg University; 2005;
  29. Ramage G, Tunney MM, Patrick S, Gorman SP, Nixon JR. Formation of Propionibacterium acnes biofilms on orthopaedic biomaterials and their susceptibility to antimicrobials. Biomaterials. 2003;24:3221–3227
  30. Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:86–93
  31. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003;36:1–11
  32. Siqueira JF, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J. 1999;32:361–369
  33. Grecca FS, Leonardo MR, da Silva LA, Tanomaru Filho M, Borges MA. Radiographic evaluation of periradicular repair after endodontic treatment of dog’s teeth with induced periradicular periodontitis. J Endod. 2001;27:610–612
  34. Sathorn C, Parashos P, Messer HH. Effectiveness of single- versus multiple-visit endodontic treatment of teeth with apical periodontitis: a systematic review and meta-analysis. Int Endod J. 2005;38:347–355
  35. Rôças IN, Baumgartner JC, Xia T, Siqueira JF. Prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. J Endod. 2006;32:1135–1138

 Supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a Brazilian governmental institution.

PII: S0099-2399(07)00006-4

doi: 10.1016/j.joen.2007.01.004

Journal of Endodontics
Volume 33, Issue 6 , Pages 667-672 , June 2007