Journal of Endodontics
Volume 32, Issue 2 , Pages 104-109 , February 2006

Prevalence of Enterococcus faecalis at Multiple Oral Sites in Endodontic Patients Using Culture and PCR

  • Christine Sedgley, PhD

      Affiliations

    • Corresponding Author InformationAddress requests for reprint to Dr. Christine Sedgley, Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Drive, Ann Arbor, MI 48109-1078.
  • ,
  • Gwendolyn Buck, BS
  • ,
  • Oliver Appelbe, BS

References 

  1. Byström A , Claesson R , Sundqvist G . The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals . Endod Dent Traumatol . 1985;1:170–175
  2. Sjögren U , Figdor D , Persson S , Sundqvist G . Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis . Int Endod J . 1997;30:297–306
  3. Siqueira JF , Rôças IN , Souto R , de Uzeda M , Colombo AP . Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections . J Endod . 2002;28:168–172
  4. Baumgartner JC , Siqueira JF , Xia T , Rôças IN . Geographical differences in bacteria detected in endodontic infections using polymerase chain reaction . J Endod . 2004;30:141–144
  5. Molander A , Reit C , Dahlén G , Kvist T . Microbiological status of root-filled teeth with apical periodontitis . Int Endod J . 1998;31:1–7
  6. Sundqvist G , Figdor D , Persson S , Sjögren U . Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment . Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 1998;85:86–93
  7. Hancock HH , Sigurdsson A , Trope M , Moiseiwitsch J . Bacteria isolated after unsuccessful endodontic treatment in a North American population . Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 2001;91:579–586
  8. Rôças IN , Siqueira JF , Santos KRN . Association of Enterococcus faecalis with different forms of periradicular diseases . J Endod . 2004;30:315–320
  9. Rôças IN , Jung IY , Lee CY , Siqueira JF . Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population . J Endod . 2004;30:504–508
  10. Siqueira JF , Rôças IN . Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment . Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 2004;97:85–94
  11. Gold OG , Jordan HV , van Houte J . The prevalence of enterococci in the human mouth and their pathogenicity in animal models . Arch Oral Biol . 1975;20:473–477
  12. Sedgley CM , Lennan SL , Clewell DB . Prevalence, phenotype and genotype of oral enterococci . Oral Microbiol Immunol . 2004;19:95–101
  13. Sedgley CM , Nagel A , Shelburne C , Clewell DB , Appelbe OK , Molander A . Quantitative real-time PCR detection of oral Enterococcus faecalis . Arch Oral Biol . 2005;50:575–583
  14. Socransky SS , Manganiello SD . The oral microbiota of man from birth to senility . J Periodontol . 1971;42:485–496
  15. Hartke A , Giard JC , Laplace JM , Auffray Y . Survival of Enterococcus faecalis in an oligotrophic microcosm (changes in morphology, development of general stress resistance, and analysis of protein synthesis) . Appl Environ Microbiol . 1998;64:4238–4245
  16. Möller AJ. Microbiological examination of root canals and periapical tissues of human teeth. Thesis Odontol Tidskr 1966; spec 74.
  17. Ng YL , Spratt D , Sriskantharajah S , Gulabivala K . Evaluation of protocols for field decontamination before bacterial sampling of root canals for contemporary microbiology techniques . J Endod . 2003;29:317–320
  18. Sedgley CM , Molander A , Flannagan SE , et al.   Virulence, phenotype and genotype characteristics of endodontic Enterococcus spp . Oral Microbiol Immunol . 2005;20:10–19
  19. Engström B . The significance of enterococci in root canal treatment . Odontologisk Revy . 1964;15:87–106
  20. Shelburne CE , Prabhu A , Gleason RM , Mullally BH , Coulter WA . Quantitation of Bacteroides forsythus in subgingival plaque (comparison of immunoassay and quantitative polymerase chain reaction) . J Micro Meth . 2000;39:97–107
  21. Rolph HJ , Lennon A , Riggio MP , et al.   Molecular identification of microorganisms from endodontic infections . J Clin Microbiol . 2001;39:3282–3289
  22. Kishen A , Chen NN , Tan L , Asundi A . Chairside sensor for rapid monitoring of Enterococcus faecalis activity . J Endod . 2004;30:872–875
  23. Papapanou PN , Abron A , Verbitsky M , et al.   Gene expression signatures in chronic and aggressive periodontitis (a pilot study) . Eur J Oral Sci . 2004;112:216–223
  24. Signoretto C , Lleo MM , Tafi MC , Canepari P . Cell wall chemical composition of Enterococcus faecalis in the viable but nonculturable state . Appl Environ Microbiol . 2000;66:1953–1959
  25. Pruzzo C , Tarsi R , Lleo MM , et al.   In vitro adhesion to human cells by viable but nonculturable Enterococcus faecalis . Curr Microbiol . 2002;45:105–110
  26. Auschill TM , Arweiler NB , Netuschil L , Brecx M , Reich E , Sculean A . Spatial distribution of vital and dead microorganisms in dental biofilms . Arch Oral Biol . 2001;46:471–476
  27. Marsh PD . Dental plaque as a microbial biofilm . Caries Res . 2004;38:204–211
  28. Sedgley CM , Clewell DB . Bacterial plasmids in the oral and endodontic microflora . Endod Topics . 2004;9:37–51
  29. Siqueira J . Taxonomic changes of bacteria associated with endodontic infections . J Endod . 2003;29:619–623
  30. Lee W , Lim S , Son HH , Bae KS . Sonicated extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes . J Endod . 2004;30:209–212
  31. Abdullah M , Ng YL , Gulabivala K , Moles DR , Spratt DA . Susceptibilities of two Enterococcus faecalis phenotypes to root canal medications . J Endod . 2005;31:30–36
  32. Cwikla SJ , Bélanger M , Giguère S , Progulske-Fox A , Vertucci FJ . Dentinal tubule disinfection using three calcium hydroxide formulations . J Endod . 2005;31:50–52
  33. Schöfer E , Bössmann K . Antimicrobial efficacy of chlorhexidine and two calcium hydroxide formulations against Enterococcus faecalis . J Endod . 2005;31:53–56
  34. Tomita H , Fujimoto S , Tanimoto K , Ike Y . Cloning and genetic and sequence analyses of the bacteriocin 21 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pPD1 . J Bacteriol . 1997;179:7843–7855
  35. Irwin RS , Whitaker S , Pratter MR , Millard CE , Tarpey JT , Corwin RW . The transiency of oropharyngeal colonization with gram-negative bacilli in residents of a skilled nursing facility . Chest . 1982;81:31–35
  36. Bentley C , Crawford JJ , Broderius CA . Analytical and physiological variability of salivary microbial counts . J Dent Res . 1988;67:1409–1413
  37. Portenier I , Waltimo TMT , Haapasalo M . Enterococcus faecalis: the root canal survivor and ‘star’ in post-treatment disease . Endod Topics . 2003;6:135–159

PII: S0099-2399(05)00062-2

doi: 10.1016/j.joen.2005.10.022

Journal of Endodontics
Volume 32, Issue 2 , Pages 104-109 , February 2006