Journal of Endodontics
Volume 32, Issue 5 , Pages 442-446 , May 2006

Role of Free Radicals and Metal Ions in Direct Current-Induced Cytotoxicity

  • Yuko Nakamura, DDS

      Affiliations

    • Department of Endodontics, Meikai, University School of Dentistry, Sakado, Saitama, Japan
    • ,
  • Keiso Takahashi, DDS, PhD

      Affiliations

    • Department of Endodontics, Meikai, University School of Dentistry, Sakado, Saitama, Japan
    • Corresponding Author InformationAddress requests for reprint to Dr. Keiso Takahashi, Department of Endodontics, Meikai University School of Dentistry, Keyaki-dai 1-1, Sakado, Saitama 350-0283, Japan.
    • ,
  • Kazue Satoh, PhD

      Affiliations

    • Dental Pharmacology, Meikai, University School of Dentistry, Sakado, Saitama, Japan
    • ,
  • Akiko Shimetani, DDS

      Affiliations

    • Department of Endodontics, Meikai, University School of Dentistry, Sakado, Saitama, Japan
    • ,
  • Hiroshi Sakagami, PhD

      Affiliations

    • Department of Anatomy, School of Medicine, Showa, University, Tokyo, Japan.
    • ,
  • Hirofumi Nishikawa, DDS, PhD

      Affiliations

    • Department of Endodontics, Meikai, University School of Dentistry, Sakado, Saitama, Japan

References 

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  2. Rajasekaran M , Hellstrom WJ , Sparks RL , Sikka SC . Sperm-damaging effects of electric current (possible role of free radicals) . Reprod Toxicol . 1994;8:427–432
  3. Holan V , Machala O , Dao T , Minowada J . Effects of direct current on T cell activity (modulation of interleukin-2 production) . Immunobiology . 1994;190:368–375
  4. Goldman R , Pollack S . Electric fields and proliferation in a chronic wound model . Bioelectromagnetics . 1996;17:450–457
  5. Fagerheim I , Lingaas E . Generation of hydroxyl radicals by polymorphonuclear neutrophils during exposure to direct current and iron . APMIS . 1996;104:493–499
  6. Berger TJ , Spadaro JA , Chapin SE , Becker RO . Electrically generated silver ions (quantitative effects on bacterial and mammalian cells) . Antimicrob Agents Chemother . 1976;9:357–358
  7. Berger TJ , Spadaro JA , Bierman R , Chapin SE , Becker RO . Antifungal properties of electrically generated metallic ions . Antimicrob Agents Chemother . 1976;10:856–860
  8. Hanawa T , Kaga M , Itoh Y , Echizenya T , Oguchi H , Ota M . Cytotoxicities of oxides, phosphates and sulphides of metals . Biomaterials . 1992;13:20–24
  9. Kinoshita N , Yamamura T , Teranuma H , et al.   Interaction between dental metals and antioxidants, assessed by cytotoxicity assay and ESR spectroscopy . Anticancer Res . 2002;22:4017–4022
  10. Wataha JC , Hanks CT , Craig RG . The in vitro effects of metal cations on eukaryotic cell metabolism . J Biomed Mater Res . 1991;25:1133–1149
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  13. Hiroi M , Tajima M , Shimojima T , Kashimata M , Miyata T , Sakagami H . Re-evaluation of the culture condition of polymorphonuclear cells for the study of apoptosis induction . Anticancer Res . 1998;18:1813–1818
  14. Sjogren G , Sletten G , Dahl JE . Cytotoxicity of dental alloys, metals, and ceramics assessed by millipore filter, agar overlay, and MTT tests . J Prosthet Dent . 2000;84:229–236
  15. Davis WL , Jacoby BH , Craig KR , Wagner G , Harrison JW . Copper-zinc superoxide dismutase activity in normal and inflamed human dental pulp tissue . J Endod . 1991;17:316–318
  16. Baumgardner KR , Sulfaro MA . The anti-inflammatory effects of human recombinant copper-zinc superoxide dismutase on pulp inflammation . J Endod . 2001;27:190–195
  17. Shimauchi H , Takayama S , Narikawa-Kiji M , Shimabukuro Y , Okada H . Production of interleukin-8 and nitric oxide in human periapical lesions . J Endod . 2001;27:749–752
  18. Shiozawa A . Characterization of reactive oxygen species generated from the mixture of NaClO and H2O2 used as root canal irrigants . J Endod . 2000;26:11–15
  19. Kawamoto K , Tsujimoto Y . Effects of the hydroxyl radical and hydrogen peroxide on tooth bleaching . J Endod . 2004;30:45–50
  20. Kashima-Tanaka M , Tsujimoto Y , Kawamoto K , Senda N , Ito K , Yamazaki M . Generation of free radicals and/or active oxygen by light or laser irradiation of hydrogen peroxide or sodium hypochlorite . J Endod . 2003;29:141–143
  21. Dahlstrom SW , Heithersay GS , Bridges TE . Hydroxyl radical activity in thermo-catalytically bleached root-filled teeth . Endod Dent Traumatol . 1997;13:119–125
  22. Becker RO , Spadaro JA . Treatment of orthopaedic infections with electrically generated silver ions. A preliminary report . J Bone Joint Surg Am . 1978;60:871–881
  23. Berger TJ , Spadaro JA , Bierman R , Chapin SE , Becker RO . Antifungal properties of electrically generated metallic ions . Antimicrob Agents Chemother . 1976;10:856–860
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PII: S0099-2399(05)00101-9

doi: 10.1016/j.joen.2005.11.010

Journal of Endodontics
Volume 32, Issue 5 , Pages 442-446 , May 2006

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