Journal of Endodontics
Volume 26, Issue 4 , Pages 217-220 , April 2000

Whole Genomic DNA Probe for Detection of Porphyromonas endodontalis

  • R. Nissan, DMD

      Affiliations

    • Dr. Nissan is assistant professor, Department of Endodontology, Temple University Dental School, Philadelphia, PA
    • Corresponding Author InformationAddress requests for reprints to Dr. Roni Nissan, Department of Endodontology, Temple University Dental School, 3223 North Broad Street, Philadelphia, PA 19140-5096
    • ,
  • S.R. Makkar, DDS

      Affiliations

    • Dr. Makkar was an endodontic resident in the Department of Endodontology, Temple University Dental School, and is now in endodontic private practice in North Boston, MA
    • ,
  • M.N. Sela, DMD, PhD

      Affiliations

    • Dr. Sela is professor and chairman, Department of Oral Biology, School of Dentistry, Hebrew University, Jerusalem, Israel
    • ,
  • R. Stevens, DDS, MS

      Affiliations

    • Dr. Stevens is professor and chairman, Department of Endodontology, Temple University Dental School, Philadelphia, PA

References 

  1. Shah H , Collins MD . Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis and Bacteroides endodontalis in a new genus Porphyromonas . Int J Syst Bacteriol . 1988;38:128–131
  2. Haapasalo M , Ranta H , Ranta K , Shah H . Black-pigmented Bacteroides spp. in human apical periodontitis . Infect Immun . 1986;53:149–153
  3. van Winkelhoff AJ , Carlee AW , de Graaff J . Bacteroides endodontalis and other black-pigmented species in odontogenic abcesses . Infect Immun . 1985;49:494–497
  4. Hashioka K , Yamasaki M , Nakane A , Horiba N , Nakamura H . The relationship between clinical symptoms and anaerobic bacteria from infected root canals . J Endodon . 1992;18:558–561
  5. Griffee M , Patterson S , Miller C , Kafrawy A , Newton C . The relationship of Bacteroides melaninogenicus to symptoms associated with pulpal necrosis . Oral Surg Oral Med Oral Pathol . 1980;50:457–461
  6. Gomes BP , Drucker DB , Lilley JD . Association of specific bacteria with some endodontic signs and symptoms . Int Endod J . 1994;27:291–298
  7. Yoshida M , Fukushima H , Yamamato K , Ogawa K , Toda T , Sagawa H . Correlation between clinical symptoms and microorganisms isolated from canals of teeth with periapical pathosis . J Endodon . 1987;13:24–28
  8. van Winkelhoff A . Serological characterization of black-pigmented Bacteroides endodontalis . Infect Immun . 1986;51:972–974
  9. Herveijer JA , Loos BG , Neiders ME . Characterization of total membrane protein of Porphyromonas endodontalis . J Endodon . 1992;18:620–624
  10. Loesche WJ , Lopatin DE , Stoll J , van Poperin N , Hujoel PP . Comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard? . J Clin Microbiol . 1992;30:418–426
  11. Strzempko MN, Simon SL, French CK, Lippke JA, Raia FF, Savitt ED, et al. Cross-reactivity study of whole genomic DNA probes for Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis . Dent Res . 1987;66:1543–1546
  12. Tay F , Liu YB , Flynn MJ , Slots J . Evaluation of a non-radioactive DNA probe for detecting Porphyromonas gingivalis in subgingival specimens . Oral Microbiol Immunol . 1992;7:344–348
  13. Suzuki K , Ikeda T , Nakamura H , Yoshimura F . Isolation and characterization of a nonpigmented variant of Porphyromonas endodontalis . Oral Microbiol Immunol . 1997;12:155–161
  14. Mayrand D , Holt SC . Biology of asaccharolytic black-pigmented Bacteroides species . Microbiol Rev . 1988;52:134–152
  15. Kilian M . Degredation of immunoglobulin A1, A2, and G by suspected principal periodontal pathogens . Infect Immun . 1981;34:757–765
  16. Shah HN , Gharbia SE . Biochemical and chemical analyses of black-pigmented Gram-negative anaerobes . FEMS Immunol Med Microbiol . 1993;6:89–96
  17. Shoshani M , Rosen G , Naor R , Sela M . Hydrolysis of the extracellular matrix proteins: fibronectin, fibrinogen and collagen IV by a protease of Porphyromonas endodontalis [IADR Abstract #2826] . J Dent Res . 1998;77:985
  18. Ogura N, Shibata Y, Kamino Y, Matsuda U, Hayakawa M, Oikawa T, et al. Stimulation of interleukin-6 production of periodontal ligament cells by Porphyromonas endodontalis lipopolysaccharide . Biochem Med Metab Biol . 1994;53:130–136
  19. Hosoya S , Matsushima K , Ohbayashi E , Yamazaki M , Shibata Y , Abiko Y . Stimulation of interleukin-1beta-independent interleukin-6 production in human dental pulp cells by lipopolysaccharide . Biochem Mol Med . 1996;59:138–143
  20. Oikawa T , Ogura N , Akiba M , Abiko Y , Takiguchi H , Izumi H . Stimulation of plasmin activity in cultured human fibroblast cells by Porphyromonas endodontalis . Int J Biochem . 1993;25:1227–1231

 This study was supported in part by the Faculty Incentive Award #466 from Temple University, Philadelphia, PA.

PII: S0099-2399(05)60957-0

doi: 10.1097/00004770-200004000-00004

Journal of Endodontics
Volume 26, Issue 4 , Pages 217-220 , April 2000

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