Journal of Endodontics
Volume 33, Issue 5 , Pages 536-540 , May 2007

Detection and Eradication of Microorganisms in Root-filled Teeth Associated With Periradicular Lesions: An In Vivo Study

  • Jörg F. Schirrmeister, Dr. med. dent.

      Affiliations

    • Department of Operative Dentistry and Periodontology, University Clinic and Dental Hospital, University of Freiburg, Freiburg, Germany
    • Corresponding Author InformationAddress requests for reprints to Dr. Jörg F. Schirrmeister, Department of Operative Dentistry and Periodontology, University School and Dental Hospital, Hugstetter Straße 55, D-79106 Freiburg, Germany.
    • ,
  • Anna-Lisa Liebenow

      Affiliations

    • Department of Operative Dentistry and Periodontology, University Clinic and Dental Hospital, University of Freiburg, Freiburg, Germany
    • ,
  • Gabriele Braun

      Affiliations

    • Department of Operative Dentistry and Periodontology, University Clinic and Dental Hospital, University of Freiburg, Freiburg, Germany
    • ,
  • Annette Wittmer

      Affiliations

    • Department Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany.
    • ,
  • Elmar Hellwig, Dr. med. dent.

      Affiliations

    • Department of Operative Dentistry and Periodontology, University Clinic and Dental Hospital, University of Freiburg, Freiburg, Germany
    • ,
  • Ali Al-Ahmad, Dr. rer. nat.

      Affiliations

    • Department of Operative Dentistry and Periodontology, University Clinic and Dental Hospital, University of Freiburg, Freiburg, Germany

References 

  1. Cheung GS, Ho MW. Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol. 2001;16:332–337
  2. Gomes BP, Pinheiro ET, Gade-Neto CR, et al. Microbiological examination of infected dental root canals. Oral Microbiol Immunol. 2004;19:71–76
  3. Gomes BP, Pinheiro ET, Sousa EL, et al. Enterococcus faecalis in dental root canals detected by culture and by polymerase chain reaction analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:247–253
  4. Hancock HH, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North Am population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;91:579–586
  5. Kaufman B, Spångberg L, Barry J, Fouad AF. Enterococcus spp. in endodontically treated teeth with and without periradicular lesions. J Endod. 2005;31:851–856
  6. Molander A, Reit C, Dahlén G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J. 1998;31:1–7
  7. Möller AJR. Microbial examination of root canals and periapical tissues of human teeth. Odontologisk Tidskrift. 1966;74(Suppl):1–380
  8. Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M. Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population. J Endod. 2000;26:593–595
  9. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J. 2001;34:429–434
  10. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003;36:1–11
  11. Rôças IN, Jung IY, Lee CY, Siqueira JF. Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population. J Endod. 2004;30:504–508
  12. Rôças IN, Siqueira JF, Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;30:315–320
  13. Rolph HJ, Lennon A, Riggio MP, et al. Molecular identification of microorganisms from endodontic infections. J Clin Microbiol. 2001;39:3282–3289
  14. Sedgley C, Nagel A, Dahlén G, Reit C, Molander A. Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals. J Endod. 2006;32:173–177
  15. Siqueira JF, Rôças IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97:85–94
  16. Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:86–93
  17. Zehnder M. Root canal irrigants. J Endod. 2006;32:389–398
  18. Byström A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985;18:35–40
  19. Yoshida T, Shibata T, Shinohara T, Gomyo S, Sekine I. Clinical evaluation of the efficacy of EDTA solution as an endodontic irrigant. J Endod. 1995;21:592–593
  20. Zehnder M, Schmidlin P, Sener B, Waltimo T. Chelation in root canal therapy reconsidered. J Endod. 2005;31:817–820
  21. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006;32:93–98
  22. White RR, Hays GL, Janer LR. Residual antimicrobial activity after canal irrigation with chlorhexidine. J Endod. 1997;23:229–231
  23. Haapasalo M, Endal U, Zandi H, Coil JM. Eradication of endodontic infection by instrumentation and irrigation solutions. Endod Topics. 2005;10:77–102
  24. Syed SA, Loesche WJ. Survival of human dental plaque flora in various transport media. Appl Microbiol. 1972;24:638–644
  25. Otten JE, Wiedmann-Al-Ahmad M, Jahnke H, Pelz K. Bacterial colonisation on different suture materials—a potential risk for intraoral dentoalveolar surgery. J Biomed Mater Res B Appl Biomater. 2005;74:627–635
  26. Al-Ahmad A, Auschill TM, Braun G, Hellwig E, Arweiler NB. Overestimation of Streptococcus mutans prevalence by nested PCR detection of the 16S rRNA gene. J Med Microbiol. 2006;55:109–113
  27. Fouad AF, Zerella J, Barry J, Spångberg LS. Molecular detection of Enterococcus species in root canals of therapy-resistant endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99:112–118
  28. Zoletti GO, Siqueira JF, Santos KR. Identification of Enterococcus faecalis in root-filled teeth with or without periradicular lesions by culture-dependent and-independent approaches. J Endod. 2006;32:722–726
  29. Schirrmeister JF, Hermanns P, Meyer KM, Goetz F, Hellwig E. Detectability of residual Epiphany and gutta-percha after root canal retreatment using a dental operating microscope and radiographs—an ex vivo study. Int Endod J. 2006;39:558–565
  30. Spratt DA. Significance of bacterial identification by molecular biology methods. Endod Topics. 2004;9:5–14
  31. Zambon JJ, Haraszthy VI. The laboratory diagnosis of periodontal infections. Periodontology 2000. 1995;7:69–82
  32. Signoretto C, Lleo MM, Tafi MC, Canepari P. Cell wall chemical composition of Enterococcus faecalis in the viable but nonculturable state. Appl Environ Microbiol. 2000;66:1953–1959
  33. Siqueira JF, Rôças IN. Exploiting molecular methods to explore endodontic infections: Part 1—current molecular technologies for microbiological diagnosis. J Endod. 2005;31:411–423
  34. Patterson SS. In vivo and in vitro studies of the effect of the disodium slat of ethylenediamine tetra-acetate on human dentine and its endodontic implications. Oral Surg Oral Med Oral Pathol. 1963;16:83–103
  35. Siqueira JF, Machado AG, Silveira RM, Lopes HP, de Uzeda M. Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, in vitro. Int Endod J. 1997;30:279–282
  36. Abdullah M, Ng YL, Gulabivala K, Moles DR, Spratt DA. Susceptibilties of two Enterococcus faecalis phenotypes to root canal medications. J Endod. 2005;31:30–36
  37. Basrani B, Santos JM, Tjaderhane L, et al. Substantive antimicrobial activity in chlorhexidine-treated human root dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;94:240–245

PII: S0099-2399(07)00067-2

doi: 10.1016/j.joen.2007.01.012

Journal of Endodontics
Volume 33, Issue 5 , Pages 536-540 , May 2007

Article Tools

* Image Usage & Resolution