Journal of Endodontics
Volume 33, Issue 7 , Pages 788-795, July 2007

Antibacterial Efficacy of Chlorhexidine Gluconate Intracanal Medication In Vivo

  • Lisane Paquette, DMD, MSc

      Affiliations

    • Department of Endodontics, University of Toronto, Toronto, Ontario, Canada
    • ,
  • Milos Legner, PhD

      Affiliations

    • Department of Oral Microbiology, University of Toronto, Toronto, Ontario, Canada.
    • ,
  • Edward D. Fillery, PhD

      Affiliations

    • Department of Oral Microbiology, University of Toronto, Toronto, Ontario, Canada.
    • ,
  • Shimon Friedman, DMD

      Affiliations

    • Department of Endodontics, University of Toronto, Toronto, Ontario, Canada
    • Corresponding Author InformationAddress requests for reprints to Dr. Shimon Friedman, Endodontics, Faculty of Dentistry, 124 Edward Street, Toronto, Ontario M5G 1G6, Canada.

published online 10 May 2007.

Abstract 

The antibacterial efficacy of intracanal medication with 2% chlorhexidine liquid (CHX) was assessed in teeth with apical periodontitis. Canals in 22 teeth were instrumented at the first session, medicated with CHX, and reaccessed after 7 to 15 days. Bacteriological samples were aspirated at the first and second sessions, before (1A, 2A) and after (1B, 2B) canal instrumentation. Viable bacterial counts were obtained by culture (CFU) and microscopy using vital dyes. Microscopic counts were higher than CFU counts. Consistently high CFU counts in 1A samples (mean, 2 × 105 μL−1 canal volume) decreased significantly (p < 0.0001) in 1B samples, increased significantly (p < 0.04) in 2A samples, and decreased in 2B samples to the level of 1B samples. Proportions of negative cultures followed the pattern of CFU counts. Intracanal medication with CHX did not reduce the bacterial concentration. Bacterial counts expressed per microliter canal volume added information beyond the counts per tooth as expressed in previous studies.

Key Words: Apical periodontitis, chlorhexidine, epifluorescence microscopy, intracanal medication, microbiology, root canal infection

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PII: S0099-2399(07)00305-6

doi:10.1016/j.joen.2005.12.017

Journal of Endodontics
Volume 33, Issue 7 , Pages 788-795, July 2007

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