Effects of Root Canal Sealers on Lipopolysaccharide-induced Expression of Cyclooxygenase-2 mRNA in Murine Macrophage Cells

Lee, Dong Hee; Kim, Na Ryoung; Lim, Bum-Soon; Lee, Yong-Keun; Hwang, Kyu-Kye; Yang, Hyeong-Cheol

doi:10.1016/j.joen.2007.07.042

« Previous Next »Journal of Endodontics
Volume 33, Issue 11 , Pages 1329-1333, November 2007

Effects of Root Canal Sealers on Lipopolysaccharide-induced Expression of Cyclooxygenase-2 mRNA in Murine Macrophage Cells

Dong Hee Lee, PhD
- Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul
Na Ryoung Kim, BS
- Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul
Bum-Soon Lim, PhD
- Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul
Yong-Keun Lee, DDS, PhD
- Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul
Kyu-Kye Hwang, PhD
- Department of Veterinary Medicine, Cheju National University, Jeju, Korea.
Hyeong-Cheol Yang, PhD
- Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul
- Address requests for reprints to Hyeong-Cheol Yang, PhD, Department of Dental Biomaterials Science, School of Dentistry, Seoul National University, 28 Yeonkun-dong, Chongro-ku, Seoul 110-749, Korea.

published online 28 September 2007.

Abstract

Cyclooxygenase-2 (COX-2) is a useful biomarker of the inflammatory potential of biomaterials in vitro. In this study we investigate the effects of soluble extracts from 3 selected root canal sealers (AH26, Sealapex, and N2 Universal) on COX-2 mRNA expression in cultured murine macrophage cells. Root canal sealers and the addition of lipopolysaccharide (LPS) both produced significant increases in COX-2 mRNA expression in RAW 264.7 macrophages. In addition, both Sealapex and N2 Universal produced a synergistic 6- to 8-fold increase in COX-2 mRNA expression, whereas AH26 did not demonstrate synergy with LPS. These results suggest that LPS and certain root canal sealers have a synergistic effect on the inflammatory responses of macrophages. Under the conditions of this in vitro study, the results suggest that one potential mechanism of periapical inflammatory reactions might be the synergistic effects of certain root canal sealers on LPS-induced COX-2 expression by macrophage cells.

Key Words: Cyclooxygenase-2, inflammation, macrophage, RAW 264.7, root canal sealers