Journal of Endodontics
Volume 34, Issue 1 , Pages 14-21, January 2008

Profiling of Radicular Cyst and Odontogenic Keratocyst Cytokine Production Suggests Common Growth Mechanisms

  • Makoto Hayashi, DDS, PhD

      Affiliations

    • Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Takafumi Ohshima, DDS, PhD

      Affiliations

    • Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Mitsuhiro Ohshima, DDS, PhD

      Affiliations

    • Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
    • Corresponding Author InformationAddress requests for reprints to Dr Mitsuhiro Ohshima, Department of Biochemistry, Nihon University School of Dentistry, 1-8-13, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.
    • ,
  • Yoko Yamaguchi, BS

      Affiliations

    • Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Hirofumi Miyata, DDS, PhD

      Affiliations

    • Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Osamu Takeichi, DDS, PhD

      Affiliations

    • Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Bunnai Ogiso, DDS, PhD

      Affiliations

    • Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Koichi Ito, DDS, PhD

      Affiliations

    • Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
    • ,
  • Arne Östman, PhD

      Affiliations

    • Cancer Center Karolinska, Department of Pathology-Oncology, Karolinska Institute, Stockholm, Sweden.
    • ,
  • Kichibee Otsuka, DDS, PhD

      Affiliations

    • Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan
    • Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan

published online 23 November 2007.

Abstract 

The aim of this study was to compare the cytokine expression profiles of cyst fluids (CFs) and tissue culture supernatants (SUPs) from 7 radicular cysts (RCs) and 7 odontogenic keratocysts (OKCs) by using Human Cytokine Antibody Array to identify the specific cytokines involved in formation and expansion of RCs and OKCs, respectively. There were significant differences in relative expression levels of IL-1β, MCP1, MIP1β, FGF-9, GDNF, HGF, IGFBP-3, Ang, IP-10, MIF, OPG, and TGF-β2 between RC-CF and OKC-CF (P < .05). On the other hand, the cytokine expression patterns of RC-SUP (HGF, IL-8, NAP-2, IL-6, TIMP-1 and 2, GRO, IP-10, and Ang) were similar to those of OKC-SUP. Only the relative expression level of GRO differed between RC-SUP and OKC-SUP (P < .05). The similarities of cytokine production by tissue cultures derived from RC and OKC indicate that the expansion mechanisms of RC and OKC might involve similar biologic mechanisms other than infection.

Key Words: Cytokine profile, odontogenic keratocyst, radicular cyst

 

PII: S0099-2399(07)00833-3

doi:10.1016/j.joen.2007.08.020

Journal of Endodontics
Volume 34, Issue 1 , Pages 14-21, January 2008