Journal of Endodontics
Volume 34, Issue 2 , Pages 186-189, February 2008

An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a Methacrylate-based Endodontic Sealer

  • Erich P. Heitman, DMD

      Affiliations

    • U.S. Army Endodontic Residency Program, Fort Gordon, Georgia
    • Corresponding Author InformationAddress requests for reprints to MAJ Erich P. Heitman, USA DENTAC, Tingay Dental Clinic, Fort Gordon, GA 30905.
    • ,
  • Anthony P. Joyce, DDS

      Affiliations

    • U.S. Army Endodontic Residency Program, Fort Gordon, Georgia
    • ,
  • James C. McPherson III, PhD

      Affiliations

    • U.S. Army Department of Clinical Investigations, Fort Gordon, Georgia.
    • ,
  • Steven Roberts, DDS

      Affiliations

    • Department of Endodontics, Medical College of Georgia, Augusta, Georgia
    • ,
  • Augustine Chuang, PhD

      Affiliations

    • U.S. Army Department of Clinical Investigations, Fort Gordon, Georgia.

published online 10 December 2007.

Abstract 

The cytotoxicity of Epiphany root canal sealer at various concentrations from 25–800 μg/mL on human periodontal ligament (HPDL) fibroblasts was evaluated at 1, 3, and 7 days. Controls included untreated cells and cells treated with the vehicle for Epiphany suspension. Fibroblast viability was assessed by 2 methods, crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates. Significant cytotoxicity against HPDL fibroblast growth by Epiphany was both time- and concentration-dependent. On day 1, 800 μg/mL, the highest concentration of Epiphany, showed significant cytotoxicity (P ≤ .001). By day 7, all concentrations greater than 25 μg/mL showed significant (P ≤ .05) loss of viability. This study demonstrated increased Epiphany cytotoxicity with an increase in concentration or exposure time.

Key Words: Cytotoxicity, Epiphany, root canal sealer

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 20.00 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0099-2399(07)00939-9

doi:10.1016/j.joen.2007.10.018

Journal of Endodontics
Volume 34, Issue 2 , Pages 186-189, February 2008

Article Tools

* Image Usage & Resolution