Journal of Endodontics
Volume 36, Issue 2 , Pages 190-202 , February 2010

Mineral Trioxide Aggregate: A Comprehensive Literature Review—Part II: Leakage and Biocompatibility Investigations

  • Mahmoud Torabinejad, DMD, MSD, PhD

      Affiliations

    • Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, California
    • Corresponding Author InformationAddress requests for reprints to Mahmoud Torabinejad, DMD, MSD, PhD, Professor and Director, Endodontic Residency Program, Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, CA 92350.
    • ,
  • Masoud Parirokh, DMD, MS

      Affiliations

    • Department of Endodontics, School of Dentistry, Oral and Dental Diseases Research Center, Neuroscience Research Center, Iranian Center for Endodontic Research, Kerman University of Medical Sciences, Kerman, Iran

References 

  1. Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature—part 2: influence of clinical factors. Int Endod J. 2008;41:6–31
  2. Torabinejad M, Pitt Ford TR. Root end filling materials. a review. Endod Dent Traumatol. 1996;12:161–178
  3. Parirokh M, Torabinejad M Mineral trioxide aggregate (MTA). a comprehensive literature review—part I: chemical, physical & antibacterial properties. J Endodo. 2010;36:16–27
  4. Torabinejad M, Watson TF, Pitt Ford TR. Sealing ability of a mineral trioxide aggregate when used as a root end filling material. J Endod. 1993;19:591–595
  5. Torabinejad M, Higa RK, McKendry DJ, Pitt Ford TR. Dye leakage of four root end filling materials: effects of blood contamination. J Endod. 1994;20:159–163
  6. Aqrabawi J. Sealing ability of amalgam, super EBA cement, and MTA when used as retrograde filling materials. Br Dent J. 2000;188:266–268
  7. Martell B, Chandler NP. Electrical and dye leakage comparison of three root-end restorative materials. Quintessence Int. 2002;33:30–34
  8. Andelin WE, Browning DF, Hsu GH, Roland DD, Torabinejad M. Microleakage of resected MTA. J Endod. 2002;28:573–574
  9. Davis JL, Jeansonne BG, Davenport WD, Gardiner D. The effect of irrigation with doxycycline or citric acid on leakage and osseous wound healing. J Endod. 2003;29:31–35
  10. Pereira CL, Cenci MS, Demarco FF. Sealing ability of MTA, Super EBA, Vitremer and amalgam as root-end filling materials. Braz Oral Res. 2004;18:317–321
  11. Asgary S, Eghbal MJ, Parirokh M. Sealing ability of a novel endodontic cement as a root-end filling material. J Biomed Mater Res. 2008;87A:706–709
  12. Tanomaru Filho M, Figueiredo FA, Tanomaru JM. Effect of different dye solutions on the evaluation of the sealing ability of mineral trioxide aggregate. Braz Oral Res. 2005;19:119–122
  13. Kubo CH, Gomes AP, Mancini MN. In vitro evaluation of apical sealing in root apex treated with demineralization agents and retrofiled with mineral trioxide aggregate through marginal dye leakage. Braz Dent J. 2005;16:187–191
  14. Vogt BF, Xavier CB, Demarco FF, Padilha MS. Dentin penetrability evaluation of three different dyes in root-end cavities filled with mineral trioxide aggregate (MTA). Braz Oral Res. 2006;20:132–136
  15. Pichardo MR, George SW, Bergeron BE, Jeansonne BG, Rutledge R. Apical leakage of root-end placed SuperEBA, MTA, and Geristore restorations in human teeth previously stored in 10% formalin. J Endod. 2006;32:956–959
  16. Tobón-Arroyave SI, Restrepo-Pérez MM, Arismendi-Echavarría JA, Velásquez-Restrepo Z, Marín-Botero ML, García-Dorado EC. Ex vivo microscopic assessment of factors affecting the quality of apical seal created by root-end fillings. Int Endod J. 2007;40:590–602
  17. Gondim E, Kim S, de Souza-Filho FJ. An investigation of microleakage from root-end fillings in ultrasonic retrograde cavities with or without finishing: a quantitative analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99:755–760
  18. Abedi HR, Van Mierlo BL, Wilder-Smith P, Torabinejad M. Effects of ultrasonic root-end cavity preparation on the root apex. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995;80:207–213
  19. Roy CO, Jeansonne BG, Gerrets TF. Effect of an acid environment on leakage of root-end filling materials. J Endod. 2001;27:7–8
  20. Wu MK, Kontakiotis EG, Wesselink PR. Decoloration of 1% methylene blue solution in contact with dental filling materials. J Dent. 1998;26:585–589
  21. Camilleri J, Pitt Ford TR. Evaluation of the effect of tracer pH on the sealing ability of glass ionomer cement and mineral trioxide aggregate. J Mater Sci Mater Med. 2008;19:2941–2948
  22. Bates CF, Carnes DL, del Rio CE. Longitudinal sealing ability of mineral trioxide aggregate as a root-end filling material. J Endod. 1996;22:575–578
  23. Yatsushiro JD, Baumgartner JC, Tinkle JS. Longitudinal study of the microleakage of two root-end filling materials using a fluid conductive system. J Endod. 1998;24:716–719
  24. Wu MK, Kontakiotis EG, Wesselink PR. Long-term seal provided by some root-end filling materials. J Endod. 1998;24:557–560
  25. Fogel HM, Peikoff MD. Microleakage of root-end filling materials. J Endod. 2001;27:456–458Erratum in: J Endod 2001;27:634
  26. Karlovic Z, Pezelj-Ribaric S, Miletic I, Jukic S, Grgurevic J, Anic I. Erbium:YAG laser versus ultrasonic in preparation of root-end cavities. J Endod. 2005;31:821–823
  27. Lamb EL, Loushine RJ, Weller RN, Kimbrough WF, Pashley DH. Effect of root resection on the apical sealing ability of mineral trioxide aggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;95:732–735
  28. De Bruyne MA, De Bruyne RJ, Rosiers L, De Moor RJ. Longitudinal study on microleakage of three root-end filling materials by the fluid transport method and by capillary flow porometry. Int Endod J. 2005;38:129–136
  29. De Bruyne MA, De Bruyne RJ, De Moor RJ. Capillary flow porometry to assess the seal provided by root-end filling materials in a standardized and reproducible way. J Endod. 2006;32:206–209
  30. Nakamichi I, Iwaku M, Fusayama T. Bovine teeth as possible substitutes in the adhesion test. J Dent Res. 1983;62:1076–1081
  31. Valois CR, Costa ED. Influence of the thickness of mineral trioxide aggregate on sealing ability of root-end fillings in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97:108–111
  32. Saghiri MA, Lotfi M, Saghiri AM, Vosoughhosseini S, Fatemi A, Shiezadeh V, et al. Effect of pH on sealing ability of white mineral trioxide aggregate as a root-end filling material. J Endod. 2008;34:1226–1229
  33. Torabinejad M, Rastegar AF, Kettering JD, Pitt Ford TR. Bacterial leakage of mineral trioxide aggregate as a root-end filling material. J Endod. 1995;21:109–112
  34. Fischer EJ, Arens DE, Miller CH. Bacterial leakage of mineral trioxide aggregate as compared with zinc-free amalgam, intermediate restorative material, and Super-EBA as a root-end filling material. J Endod. 1998;24:176–179
  35. Adamo HL, Buruiana R, Schertzer L, Boylan RJ. A comparison of MTA, Super-EBA, composite and amalgam as root-end filling materials using a bacterial microleakage model. Int Endod J. 1999;32:197–203
  36. Scheerer SQ, Steiman HR, Cohen J. A comparative evaluation of three root-end filling materials: an in vitro leakage study using Prevotella nigrescens. J Endod. 2001;27:40–42
  37. Mangin C, Yesilsoy C, Nissan R, Stevens R. The comparative sealing ability of hydroxyapatite cement, mineral trioxide aggregate, and super ethoxybenzoic acid as root-end filling materials. J Endod. 2003;29:261–264
  38. Maltezos C, Glickman GN, Ezzo P, He J. Comparison of the sealing of Resilon, Pro Root MTA, and Super-EBA as root-end filling materials: a bacterial leakage study. J Endod. 2006;32:324–327
  39. Montellano AM, Schwartz SA, Beeson TJ. Contamination of tooth-colored mineral trioxide aggregate used as a root-end filling material: a bacterial leakage study. J Endod. 2006;32:452–455
  40. Ferk Luketić S, Malcić A, Jukić S, Anić I, Segović S, Kalenić S. Coronal microleakage of two root-end filling materials using a polymicrobial marker. J Endod. 2008;34:201–203
  41. Tang HM, Torabinejad M, Kettering JD. Leakage evaluation of root end filling materials using endotoxin. J Endod. 2002;28:5–7
  42. Torabinejad M, Ung B, Kettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod. 1990;16:566–569
  43. Parirokh M, Askarifard S, Mansouri S, Haghdoost AA, Raoof M, Torabinejad M. Effect of phosphate buffer saline on coronal leakage of mineral trioxide aggregate. J Oral Sci. 2009;51:187–191
  44. Fernández-Yáñez Sánchez A, Leco-Berrocal MI, Martínez-González JM. Metaanalysis of filler materials in periapical surgery. Med Oral Patol Oral Cir Bucal. 2008;13:E180–E185
  45. Bryan EB, Woollard G, Mitchell WC. Nonsurgical repair of furcal perforations: a literature review. Gen Dent. 1999;47:274–278
  46. Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod. 1993;19:541–544
  47. Nakata TT, Bae KS, Baumgartner JC. Perforation repair comparing mineral trioxide aggregate and amalgam using an anaerobic bacterial leakage model. J Endod. 1998;24:184–186
  48. Daoudi MF, Saunders WP. In vitro evaluation of furcal perforation repair using mineral trioxide aggregate or resin modified glass ionomer cement with and without the use of the operating microscope. J Endod. 2002;28:512–515
  49. Weldon JK, Pashley DH, Loushine RJ, Weller RN, Kimbrough WF. Sealing ability of mineral trioxide aggregate and super-EBA when used as furcation repair materials: a longitudinal study. J Endod. 2002;28:467–470
  50. Hardy I, Liewehr FR, Joyce AP, Agee K, Pashley DH. Sealing ability of One-Up Bond and MTA with and without a secondary seal as furcation perforation repair materials. J Endod. 2004;30:658–661
  51. Ferris DM, Baumgartner JC. Perforation repair comparing two types of mineral trioxide aggregate. J Endod. 2004;30:422–424
  52. Tsatsas DV, Meliou HA, Kerezoudis NP. Sealing effectiveness of materials used in furcation perforation in vitro. Int Dent J. 2005;55:133–141
  53. Hamad HA, Tordik PA, McClanahan SB. Furcation perforation repair comparing gray and white MTA: a dye extraction study. J Endod. 2006;32:337–340
  54. De-Deus G, Petruccelli V, Gurgel-Filho E, Coutinho-Filho T. MTA versus Portland cement as repair material for furcal perforations: a laboratory study using a polymicrobial leakage model. Int Endod J. 2006;39:293–298
  55. Hashem AA, Hassanien EE, ProRoot MTA. MTA-Angelus and IRM used to repair large furcation perforations: sealability study. J Endod. 2008;34:59–61
  56. Zou L, Liu J, Yin S, Li W, Xie J. In vitro evaluation of the sealing ability of MTA used for the repair of furcation perforations with and without the use of an internal matrix. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105:e61–e65
  57. Shemesh H, Souza EM, Wu MK, Wesselink PR. Glucose reactivity with filling materials as a limitation for using the glucose leakage model. Int Endod J. 2008;41:869–872
  58. Tselnik M, Baumgartner JC, Marshall JG. Bacterial leakage with mineral trioxide aggregate or a resin-modified glass ionomer used as a coronal barrier. J Endod. 2004;30:782–784
  59. John AD, Webb TD, Imamura G, Goodell GG. Fluid flow evaluation of Fuji Triage and gray and white ProRoot mineral trioxide aggregate intraorifice barriers. J Endod. 2008;34:830–832
  60. Barrieshi-Nusair KM, Hammad HM. Intracoronal sealing comparison of mineral trioxide aggregate and glass ionomer. Quintessence Int. 2005;36:539–545
  61. Jenkins S, Kulild J, Williams K, Lyons W, Lee C. Sealing ability of three materials in the orifice of root canal systems obturated with gutta-percha. J Endod. 2006;32:225–227
  62. Zakizadeh P, Marshall SJ, Hoover CI, et al. A novel approach in assessment of coronal leakage of intraorifice barriers: a saliva leakage and micro-computed tomographic evaluation. J Endod. 2008;34:871–875
  63. Stefopoulos S, Tsatsas DV, Kerezoudis NP, Eliades G. Comparative in vitro study of the sealing efficiency of white vs grey ProRoot mineral trioxide aggregate formulas as apical barriers. Dent Traumatol. 2008;24:207–213
  64. Martin RL, Monticelli F, Brackett WW, et al. Sealing properties of mineral trioxide aggregate orthograde apical plugs and root fillings in an in vitro apexification model. J Endod. 2007;33:272–275
  65. de Leimburg ML, Angeretti A, Ceruti P, Lendini M, Pasqualini D, Berutti E. MTA obturation of pulpless teeth with open apices: bacterial leakage as detected by polymerase chain reaction assay. J Endod. 2004;30:883–886
  66. Al-Kahtani A, Shostad S, Schifferle R, Bhambhani S. In-vitro evaluation of microleakage of an orthograde apical plug of mineral trioxide aggregate in permanent teeth with simulated immature apices. J Endod. 2005;31:117–119
  67. Vizgirda PJ, Liewehr FR, Patton WR, McPherson JC, Buxton TB. A comparison of laterally condensed gutta-percha, thermoplasticized gutta-percha, and mineral trioxide aggregate as root canal filling materials. J Endod. 2004;30:103–106
  68. Al-Hezaimi K, Naghshbandi J, Oglesby S, Simon JH, Rotstein I. Human saliva penetration of root canals obturated with two types of mineral trioxide aggregate cements. J Endod. 2005;31:453–456
  69. Bogen G. A comparison of laterally condensed gutta-percha, thermoplasticized gutta-percha, and mineral trioxide aggregate as root canal filling materials. J Endod. 2004;30:826;author reply 826
  70. Abuabara A, Santos AJ, Aguiar FH, Lovadino JR. Evaluation of microleakage in human, bovine and swine enamels. Braz Oral Res. 2004;18:312–316
  71. Camargo CH, Siviero M, Camargo SE, de Oliveira SH, Carvalho CA, Valera MC. Topographical, diametral, and quantitative analysis of dentin tubules in the root canals of human and bovine teeth. J Endod. 2007;33:4226
  72. Camargo CH, Bernardineli N, Valera MC, et al. Vehicle influence on calcium hydroxide pastes diffusion in human and bovine teeth. Dent Traumatol. 2006;22:302–306
  73. Camargo SE, Valera MC, Camargo CH, Gasparoto Mancini MN, Menezes MM. Penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique. J Endod. 2007;33:1074–1077
  74. Torabinejad M, Smith PW, Kettering JD, Pitt Ford TR. Comparative investigation of marginal adaptation of mineral trioxide aggregate and other commonly used root-end filling materials. J Endod. 1995;21:295–299
  75. Gondim E, Zaia AA, Gomes BP, Ferraz CC, Teixeira FB, Souza-Filho FJ. Investigation of the marginal adaptation of root-end filling materials in root-end cavities prepared with ultrasonic tips. Int Endod J. 2003;36:491–499
  76. Shipper G, Grossman ES, Botha AJ, Cleaton-Jones PE. Marginal adaptation of mineral trioxide aggregate (MTA) compared with amalgam as a root-end filling material: a low-vacuum (LV) versus high-vacuum (HV) SEM study. Int Endod J. 2004;37:325–336
  77. Peters CI, Peters OA. Occlusal loading of EBA and MTA root-end fillings in a computer-controlled masticator: a scanning electron microscopic study. Int Endod J. 2002;35:22–29
  78. Gandolfi MG, Sauro S, Mannocci F, et al. New tetrasilicate cements as retrograde filling material: an in vitro study on fluid penetration. J Endod. 2007;33:742–745
  79. Xavier CB, Weismann R, de Oliveira MG, Demarco FF, Pozza DH. Root-end filling materials: apical microleakage and marginal adaptation. J Endod. 2005;31:539–542
  80. de Souza EB, de Amorim CV, Marques JL. Effect of diode laser irradiation on the apical sealing of MTA retrofillings. Braz Oral Res. 2006;20:231–234
  81. Winik R, Araki AT, Negrão JA, Bello-Silva MS, Lage-Marques JL. Sealer penetration and marginal permeability after apicoectomy varying retrocavity preparation and retrofilling material. Braz Dent J. 2006;17:323–327
  82. Shemesh H. To the editor: Hashem and Hassanien “ProRoot MTA, MTA-Angelus, and IRM used to repair large furcation perforations: sealability study”. J Endod. 2008;34:512;author reply 512–3
  83. Pelliccioni GA, Vellani CP, Gatto MR, Gandolfi MG, Marchetti C, Prati C. Proroot mineral trioxide aggregate cement used as a retrograde filling without addition of water: an in vitro evaluation of its microleakage. J Endod. 2007;33:1082–1085
  84. Shahi S, Rahimi S, Yavari HR, Shakouie S, Nezafati S, Abdolrahimi M. Sealing ability of white and gray mineral trioxide aggregate mixed with distilled water and 0.12% chlorhexidine gluconate when used as root-end filling materials. J Endod. 2007;33:1429–1432
  85. Rahimi S, Shahi S, Lotfi M, Yavari HR, Charehjoo ME. Comparison of microleakage with three different thicknesses of mineral trioxide aggregate as root-end filling material. J Oral Sci. 2008;50:273–277
  86. Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TR. The constitution of mineral trioxide aggregate. Dent Mater. 2005;21:297–303
  87. Fridland M, Rosado R. Mineral trioxide aggregate (MTA) solubility and porosity with different water-to-powder ratios. J Endod. 2003;29:814–817
  88. De Bruyne MA, De Moor RJ. Influence of cracks on leakage and obturation efficiency of root-end filling materials after ultrasonic preparation: an in vitro evaluation. Quintessence Int. 2008;39:685–692
  89. Nandini S, Ballal S, Kandaswamy D. Influence of glass-ionomer cement on the interface and setting reaction of mineral trioxide aggregate when used as a furcal repair material using laser Raman spectroscopic analysis. J Endod. 2007;33:167–172
  90. Uyanik MO, Nagas E, Sahin C, Dagli F, Cehreli ZC. Effects of different irrigation regimens on the sealing properties of repaired furcal perforations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:e91–e95
  91. Smith JB, Loushine RJ, Weller RN, et al. Metrologic evaluation of the surface of white MTA after the use of two endodontic irrigants. J Endod. 2007;33:463–467
  92. Rafter M. Apexification: a review. Dent Traumatol. 2005;21:1–8
  93. Andreasen JO, Farik B, Munksgaard EC. Long-term calcium hydroxide as a root canal dressing may increase risk of root fracture. Dent Traumatol. 2002;18:134–137
  94. Hatibović-Kofman S, Raimundo L, Zheng L, Chong L, Friedman M, Andreasen JO. Fracture resistance and histological findings of immature teeth treated with mineral trioxide aggregate. Dent Traumatol. 2008;24:272–276
  95. Bramante CM, Luna-Cruz SM, Sipert CR, et al. Alveolar mucosa necrosis induced by utilisation of calcium hydroxide as root canal dressing. Int Dent J. 2008;58:81–85
  96. Shabahang S, Torabinejad M. Treatment of teeth with open apices using mineral trioxide aggregate. Pract Periodontics Aesthet Dent. 2000;12:315–320
  97. Matt GD, Thorpe JR, Strother JM, McClanahan SB. Comparative study of white and gray mineral trioxide aggregate (MTA) simulating a one- or two-step apical barrier technique. J Endod. 2004;30:876–879
  98. Saghiri MA, Lotfi M, Saghiri AM, Vosoughhosseini S, Aeinehchi M, Ranjkesh B. Scanning electron micrograph and surface hardness of mineral trioxide aggregate in the presence of alkaline pH. J Endod. 2009;35:706–710
  99. Hachmeister DR, Schindler WG, Walker WA, Thomas DD. The sealing ability and retention characteristics of mineral trioxide aggregate in a model of apexification. J Endod. 2002;28:386–390
  100. Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod. 2005;31:97–100
  101. Bozeman TB, Lemon RR, Eleazer PD. Elemental analysis of crystal precipitate from gray and white MTA. J Endod. 2006;32:425–428
  102. Lawley GR, Schindler WG, Walker WA, Kolodrubetz D. Evaluation of ultrasonically placed MTA and fracture resistance with intracanal composite resin in a model of apexification. J Endod. 2004;30:167–172
  103. Kim US, Shin SJ, Chang SW, Yoo HM, Oh TS, Park DS. In vitro evaluation of bacterial leakage resistance of an ultrasonically placed mineral trioxide aggregate orthograde apical plug in teeth with wide open apexes: a preliminary study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:e52–e56
  104. Aminoshariae A, Hartwell GR, Moon PC. Placement of mineral trioxide aggregate using two different techniques. J Endod. 2003;29:679–682
  105. Chogle S, Mickel AK, Chan DM, Huffaker K, Jones JJ. Intracanal assessment of mineral trioxide aggregate setting and sealing properties. Gen Dent. 2007;55:306–311
  106. Bidar M, Moradi S, Jafarzadeh H, Bidad S. Comparative SEM study of the marginal adaptation of white and grey MTA and Portland cement. Aust Endod J. 2007;33:2–6
  107. Costa AT, Konrath F, Dedavid B, Weber JB, de Oliveira MG. Marginal adaptation of root-end filling materials: an in vitro study with teeth and replicas. J Contemp Dent Pract. 2009;10:75–82
  108. de Martins GR, Carvalho CA, Valera MC, de Oliveira LD, Buso L, Carvalho AS. Sealing ability of castor oil polymer as a root-end filling material. J Appl Oral Sci. 2009;17:220–223
  109. Lolayekar N, Baht SS, Hegde S. Sealing ability of ProRoot MTA and MTA-Angelus simulating a one-Step apical barrier technique- an in vitro study. J Clin Pediatr Dent. 2009;33:305–310
  110. Bortoluzzi EA, Broon NJ, Bramante CM, Garcia RB, de Moraes IG, Bernardineli N. Sealing ability of MTA and radiopaque Portland cement with or without calcium chloride for root-end filling. J Endod. 2006;32:897–900
  111. De-Deus G, Reis C, Brandão C, Fidel S, Fidel RA. The ability of Portland cement, MTA, and MTA Bio to prevent through-and-through fluid movement in repaired furcal perforations. J Endod. 2007;33:1374–1377
  112. Reyes-Carmona JF, Felippe MS, Felippe WT. Biomineralization ability and interaction of mineral trioxide aggregate and white portland cement with dentin in a phosphate-containing fluid. J Endod. 2009;35:731–736
  113. Budig CG, Eleazer PD. In vitro comparison of the setting of dry ProRoot MTA by moisture absorbed through the root. J Endod. 2008;34:712–714
  114. Hong ST, Bae KS, Baek SH, Kum KY, Lee W. Microleakage of accelerated mineral trioxide aggregate and Portland cement in an in vitro apexification model. J Endod. 2008;34:56–58
  115. Holt DM, Watts JD, Beeson TJ, Kirkpatrick TC, Rutledge RE. The anti-microbial effect against enterococcus faecalis and the compressive strength of two types of mineral trioxide aggregate mixed with sterile water or 2% chlorhexidine liquid. J Endod. 2007;33:844–847
  116. Weller RN, Tay KC, Garrett LV, et al. Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid. Int Endod J. 2008;41:977–986
  117. Huffman BP, Mai S, Pinna L, et al. Dislocation resistance of ProRoot Endo Sealer, a calcium silicate-based root canal sealer, from radicular dentine. Int Endod J. 2009;42:34–46
  118. Shahravan A, Haghdoost AA, Adl AR, Rahimi H, Shadifar F. Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod. 2007;33:96–105
  119. Yildirim T, Oruçoğlu H, Cobankara FK. Long-term evaluation of the influence of smear layer on the apical sealing ability of MTA. J Endod. 2008;34:1537–1540
  120. Lee YL, Lin FH, Wang WH, Ritchie HH, Lan WH, Lin CP. Effects of EDTA on the hydration mechanism of mineral trioxide aggregate. J Dent Res. 2007;86:534–538
  121. Jacobovitz M, Vianna ME, Pandolfelli VC, Oliveira IR, Rossetto HL, Gomes BP. Root canal filling with cements based on mineral aggregates: an in vitro analysis of bacterial microleakage. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:140–144
  122. Orosco FA, Bramante CM, Garcia RB, Bernadineli N, Moraes IG. Sealing ability of grar MTA AngelusTM, CPM TM and MBPc used as apical plugs. J Appl Oral Sci. 2008;16:50–54
  123. Camilleri J, Montesin FE, Di Silvio L, Pitt Ford TR. The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use. Int Endod J. 2005;38:834–842
  124. Camilleri J, Montesin FE, Papaioannou S, McDonald F, Pitt Ford TR. Biocompatibility of two commercial forms of mineral trioxide aggregate. Int Endod J. 2004;37:699–704
  125. Saidon J, He J, Zhu Q, Safavi K, Spångberg LS. Cell and tissue reactions to mineral trioxide aggregate and Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;95:483–489
  126. De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J. 2005;38:604–609
  127. Kim EC, Lee BC, Chang HS, Lee W, Hong CU, Min KS. Evaluation of the radiopacity and cytotoxicity of Portland cements containing bismuth oxide. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105:e54–e57
  128. Bodrumlu E. Biocompatibility of retrograde root filling materials: a review. Aust Endod J. 2008;34:30–35
  129. Kettering JD, Torabinejad M. Investigation of mutagenicity of mineral trioxide aggregate and other commonly used root-end filling materials. J Endod. 1995;21:537–542
  130. Asrari M, Lobner D. In vitro neurotoxic evaluation of root-end-filling materials. J Endod. 2003;29:743–746
  131. Abbasipour F, Rastqar A, Bakhtiar H, Khalilkhani H, Aeinehchi M, Janahmadi M. The nociceptive and anti-nociceptive effects of white mineral trioxide aggregate. Int Endod J. 2009;42:794–801
  132. Masuda YM, Wang X, Hossain M, et al. Evaluation of biocompatibility of mineral trioxide aggregate with an improved rabbit ear chamber. J Oral Rehabil. 2005;32:145–150
  133. Tunca YM, Aydin C, Ozen T, Seyrek M, Ulusoy HB, Yildiz O. The effect of mineral trioxide aggregate on the contractility of the rat thoracic aorta. J Endod. 2007;33:823–826
  134. Torabinejad M, Hong CU, Pitt Ford TR, Kettering JD. Cytotoxicity of four root end filling materials. J Endod. 1995;21:489–492
  135. Osorio RM, Hefti A, Vertucci FJ, Shawley AL. Cytotoxicity of endodontic materials. J Endod. 1998;24:91–96
  136. Keiser K, Johnson CC, Tipton DA. Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts. J Endod. 2000;26:288–291
  137. Zhu Q, Haglund R, Safavi KE, Spangberg LS. Adhesion of human osteoblasts on root-end filling materials. J Endod. 2000;26:404–406
  138. Thomson TS, Berry JE, Somerman MJ, Kirkwood KL. Cementoblasts maintain expression of osteocalcin in the presence of mineral trioxide aggregate. J Endod. 2003;29:407–412
  139. Pérez AL, Spears R, Gutmann JL, Opperman LA. Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot MTA and White MTA. Int Endod J. 2003;36:564–570
  140. Pistorius A, Willershausen B, Briseño Marroquin B. Effect of apical root-end filling materials on gingival fibroblasts. Int Endod J. 2003;36:610–615
  141. Fayad MI, Hawkinson R, Daniel J, Hao J. The effect of CO2 laser irradiation on PDL cell attachment to resected root surfaces. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97:518–523
  142. Bonson S, Jeansonne BG, Lallier TE. Root-end filling materials alter fibroblast differentiation. J Dent Res. 2004;83:408–413
  143. Balto HA. Attachment and morphological behavior of human periodontal ligament fibroblasts to mineral trioxide aggregate: a scanning electron microscope study. J Endod. 2004;30:25–29
  144. Pelliccioni GA, Ciapetti G, Cenni E, et al. Evaluation of osteoblast-like cell response to Proroot MTA (mineral trioxide aggregate) cement. J Mater Sci Mater Med. 2004;15:167–173
  145. Koulaouzidou EA, Papazisis KT, Economides NA, Beltes P, Kortsaris AH. Antiproliferative effect of mineral trioxide aggregate, zinc oxide-eugenol cement, and glass-ionomer cement against three fibroblastic cell lines. J Endod. 2005;31:44–46
  146. Nakayama A, Ogiso B, Tanabe N, Takeichi O, Matsuzaka K, Inoue T. Behaviour of bone marrow osteoblast-like cells on mineral trioxide aggregate: morphology and expression of type I collagen and bone-related protein mRNAs. Int Endod J. 2005;38:203–210
  147. Huang TH, Yang CC, Ding SJ, Yan M, Chou MY, Kao CT. Biocompatibility of human osteosarcoma cells to root end filling materials. J Biomed Mater Res B Appl Biomater. 2005;72:140–145
  148. Moghaddame-Jafari S, Mantellini MG, Botero TM, McDonald NJ, Nör JE. Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro. J Endod. 2005;31:387–391
  149. Vajrabhaya LO, Korsuwannawong S, Jantarat J, Korre S. Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:e48–e50
  150. Al-Rabeah E, Perinpanayagam H, MacFarland D. Human alveolar bone cells interact with ProRoot and tooth-colored MTA. J Endod. 2006;32:872–875
  151. Kao CT, Tsai CH, Huang TH. Tissue and cell reactions to implanted root-end filling materials. J Mater Sci Mater Med. 2006;17:841–847
  152. Takita T, Hayashi M, Takeichi O, et al. Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells. Int Endod J. 2006;39:415–422
  153. Melegari KK, Botero TM, Holland GR. Prostaglandin E production and viability of cells cultured in contact with freshly mixed endodontic materials. Int Endod J. 2006;39:357–362
  154. Oviir T, Pagoria D, Ibarra G, Geurtsen W. Effects of gray and white mineral trioxide aggregate on the proliferation of oral keratinocytes and cementoblasts. J Endod. 2006;32:210–213
  155. Souza NJ, Justo GZ, Oliveira CR, Haun M, Bincoletto C. Cytotoxicity of materials used in perforation repair tested using the V79 fibroblast cell line and the granulocyte-macrophage progenitor cells. Int Endod J. 2006;39:40–47
  156. Yoshimine Y, Ono M, Akamine A. In vitro comparison of the biocompatibility of mineral trioxide aggregate, 4META/MMA-TBB resin, and intermediate restorative material as root-end-filling materials. J Endod. 2007;33:1066–1069
  157. Gorduysus M, Avcu N, Gorduysus O, et al. Cytotoxic effects of four different endodontic materials in human periodontal ligament fibroblasts. J Endod. 2007;33:1450–1454
  158. Tani-Ishii N, Hamada N, Watanabe K, Tujimoto Y, Teranaka T, Umemoto T. Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. J Endod. 2007;33:836–839
  159. Guven G, Cehreli ZC, Ural A, Serdar MA, Basak F. Effect of mineral trioxide aggregate cements on transforming growth factor beta1 and bone morphogenetic protein production by human fibroblasts in vitro. J Endod. 2007;33:447–450
  160. Coon D, Gulati A, Cowan C, He J. The role of cyclooxygenase-2 (COX-2) in inflammatory bone resorption. J Endod. 2007;33:432–436
  161. de Souza Costa CA, Duarte PT, de Souza PP, Giro EM, Hebling J. Cytotoxic effects and pulpal response caused by a mineral trioxide aggregate formulation and calcium hydroxide. Am J Dent. 2008;21:255–261
  162. Laurent P, Camps J, De Méo M, Déjou J, About I. Induction of specific cell responses to a Ca(3)SiO(5)-based posterior restorative material. Dent Mater. 2008;24:1486–1494
  163. Yasuda Y, Ogawa M, Arakawa T, Kadowaki T, Saito T. The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study. J Endod. 2008;34:1057–1060
  164. Koh ET, McDonald F, Pitt Ford TR, Torabinejad M. Cellular response to Mineral Trioxide Aggregate. J Endod. 1998;24:543–547
  165. Camp MA, Jeansonne BG, Lallier T. Adhesion of human fibroblasts to root-end-filling materials. J Endod. 2003;29:602–607
  166. Haglund R, He J, Jarvis J, Safavi KE, Spångberg LS, Zhu Q. Effects of root-end filling materials on fibroblasts and macrophages in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;95:739–745
  167. Asgary S, Parirokh M, Eghbal MJ, Brink F. Chemical differences between white and gray mineral trioxide aggregate. J Endod. 2005;31:101–103
  168. Asgary S, Parirokh M, Eghbal MJ, Stowe S, Brink F. A qualitative X-ray analysis of white and grey mineral trioxide aggregate using compositional imaging. J Mater Sci Mater Med. 2006;17:187–191
  169. Hakki SS, Bozkurt SB, Hakki EE, Belli S. Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts. J Endod. 2009;35:513–519
  170. Sepet E, Pinar A, Ilhan B, Ulukapi I, Bilir A, Tuna S. Cytotoxic effects of calcium hydroxide and mineral trioxide aggregate on 3t3 fibroblast cell line in vitro. Quintessence Int. 2009;40:e55–e61
  171. Chen CL, Huang TH, Ding SJ, Shie MY, Kao CT. Comparison of calcium and silicate cement and mineral trioxide aggregate biologic effects and bone markers expression in MG63 cells. J Endod. 2009;35:682–685
  172. Ribeiro DA, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate. Braz Oral Res. 2005;19:183–187
  173. Ribeiro DA, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. Ex vivo biocompatibility tests of regular and white forms of mineral trioxide aggregate. Int Endod J. 2006;39:26–30
  174. da Silva GN, Braz MG, de Camargo EA, Salvadori DM, Ribeiro DA. Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:e50–e54
  175. Ribeiro DA, Sugui MM, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:258–261
  176. Braz MG, Camargo EA, Salvadori DM, Marques ME, Ribeiro DA. Evaluation of genetic damage in human peripheral lymphocytes exposed to mineral trioxide aggregate and Portland cements. J Oral Rehabil. 2006;33:234–239
  177. De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J. 2005;38:604–609
  178. Koulaouzidou EA, Economides N, Beltes P, Geromichalos G, Papazisis K. In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus. J Oral Sci. 2008;50:397–402
  179. Miranda RB, Fidel SR, Boller MA. L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay. Braz Dent J. 2009;20:22–26
  180. de Menezes JV, Takamori ER, Bijella MF, Granjeiro JM. In vitro toxicity of MTA compared with other primary teeth pulpotomy agents. J Clin Pediatr Dent. 2009;33:217–221
  181. Camargo SE, Camargo CH, Hiller KA, Rode SM, Schweikl H, Schmalz G. Cytotoxicity and genotoxicity of pulp capping materials in two cell lines. Int Endod J. 2009;42:227–237
  182. Kogan P, He J, Glickman GN, Watanabe I. The effects of various additives on setting properties of MTA. J Endod. 2006;32:569–572
  183. Wiltbank KB, Schwartz SA, Schindler WG. Effect of selected accelerants on the physical properties of mineral trioxide aggregate and Portland cement. J Endod. 2007;33:1235–1238
  184. Ber BS, Hatton JF, Stewart GP. Chemical modification of proroot mta to improve handling characteristics and decrease setting time. J Endod. 2007;33:1231–1234
  185. Huang TH, Shie MY, Kao CT, Ding SJ. The effect of setting accelerator on properties of mineral trioxide aggregate. J Endod. 2008;34:590–593
  186. Ding SJ, Kao CT, Shie MY, Hung C, Huang TH. The physical and cytological properties of white MTA mixed with Na2HPO4 as an accelerant. J Endod. 2008;34:748–751
  187. Stowe TJ, Sedgley CM, Stowe B, Fenno JC. The effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored ProRoot mineral trioxide aggregate. J Endod. 2004;30:429–431
  188. Hong ST, Bae KS, Baek SH, Kum KY, Lee W. Microleakage of accelerated mineral trioxide aggregate and Portland cement in an in vitro apexification model. J Endod. 2008;34:56–58
  189. Ding SJ, Kao CT, Shie MY, Hung C, Huang TH. The physical and cytological properties of white MTA mixed with Na2HPO4 as an accelerant. J Endod. 2008;34:748–751
  190. Jafarnia B, Jiang J, He J, Wang YH, Safavi KE, Zhu Q. Evaluation of cytotoxicity of MTA employing various additives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:739–744
  191. Min KS, Yang SH, Kim EC. The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells. J Endod. 2009;35:847–851
  192. Hernandez EP, Botero TM, Mantellini MG, McDonald NJ, Nör JE. Effect of ProRoot MTA mixed with chlorhexidine on apoptosis and cell cycle of fibroblasts and macrophages in vitro. Int Endod J. 2005;38:137–143
  193. Karimjee CK, Koka S, Rallis DM, Gound TG. Cellular toxicity of mineral trioxide aggregate mixed with an alternative delivery vehicle. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:e115–e120
  194. Abdullah D, Ford TR, Papaioannou S, Nicholson J, McDonald F. An evaluation of accelerated Portland cement as a restorative material. Biomaterials. 2002;23:4001–4010
  195. Koh ET, Torabinejad M, Pitt Ford TR, Brady K, McDonald F. Mineral trioxide aggregate stimulates a biological response in human osteoblasts. J Biomed Mater Res. 1997;37:432–439
  196. Mitchell PJ, Pitt Ford TR, Torabinejad M, McDonald F. Osteoblast biocompatibility of mineral trioxide aggregate. Biomaterials. 1999;20:167–173
  197. Huang TH, Ding SJ, Hsu TC, Kao CT. Effects of mineral trioxide aggregate (MTA) extracts on mitogen-activated protein kinase activity in human osteosarcoma cell line (U2OS). Biomaterials. 2003;24:3909–3913
  198. Huang TH, Yang CC, Ding SJ, Yeng M, Kao CT, Chou MY. Inflammatory cytokines reaction elicited by root-end filling materials. J Biomed Mater Res B Appl Biomater. 2005;73:123–128
  199. Rezende TM, Vargas DL, Cardoso FP, Sobrinho AP, Vieira LQ. Effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages. Int Endod J. 2005;38:896–903
  200. Tomson PL, Grover LM, Lumley PJ, Sloan AJ, Smith AJ, Cooper PR. Dissolution of bio-active dentine matrix components by mineral trioxide aggregate. J Dent. 2007;35:636–642
  201. Rezende TM, Vieira LQ, Cardoso FP, et al. The effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by M1 and M2 macrophages. Int Endod J. 2007;40:603–611
  202. Simon S, Cooper P, Smith A, Picard B, Ifi CN, Berdal A. Evaluation of a new laboratory model for pulp healing: preliminary study. Int Endod J. 2008;41:781–790
  203. Deller-Quinn M, Perinpanayagam H. Osteoblast expression of cytokines is altered on MTA surfaces. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:302–307
  204. Minamikawa H, Deyama Y, Nakamura K, et al. Effect of mineral trioxide aggregate on rat clonal dental pulp cells: expression of cyclooxygenase-2 mRNA and inflammation-related protein via nuclear factor kappa B signaling system. J Endod. 2009;35:843–846
  205. Andelin WE, Shabahang S, Wright K, Torabinejad M. Identification of hard tissue after experimental pulp capping using dentin sialoprotein (DSP) as a marker. J Endod. 2003;29:646–650
  206. Perinpanayagam H, Al-Rabeah E. Osteoblasts interact with MTA surfaces and express Runx2. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:590–596
  207. Masuda-Murakami Y, Kobayashi M, Wang X, et al. Effectsof mineral trioxide aggregate on the differentiation of rat dental pulp cells. Acta Histochem. 2009 Jun 26;[Epub ahead of print]
  208. Ham KA, Witherspoon DE, Gutmann JL, Ravindranath S, Gait TC, Opperman LA. Preliminary evaluation of BMP-2 expression and histological characteristics during apexification with calcium hydroxide and mineral trioxide aggregate. J Endod. 2005;31:275–279
  209. Kuratate M, Yoshiba K, Shigetani Y, Yoshiba N, Ohshima H, Okiji T. Immunohistochemical analysis of nestin, osteopontin, and proliferating cells in the reparative process of exposed dental pulp capped with mineral trioxide aggregate. J Endod. 2008;34:970–974
  210. He WX, Niu ZY, Zhao SL, Jin WL, Gao J, Smith AJ. TGF-beta activated Smad signalling leads to a Smad3-mediated down-regulation of DSPP in an odontoblast cell line. Arch Oral Biol. 2004;49:911–918
  211. Guichard J, Varet B, Hermine O. Transforming growth factor inhibits erythropoiesis by blocking proliferation and accelerating differentiation of erythroid progenitors. Exp Hematol. 2000;28:885–894
  212. Zermati Y, Fichelson S, Valensi F, et al. Transforming growth factor inhibits erythropoiesis by blocking proliferation and accelerating differentiation of erythroid progenitors. Exp Hematol. 2000;28:885–894
  213. He WX, Niu ZY, Zhao SL, Smith AJ. Smad protein mediated transforming growth factor beta1 induction of apoptosis in the MDPC-23 odontoblast-like cell line. Arch Oral Biol. 2005;50:929–936
  214. Hunter GK, Hauschka PV, Poole AR, Rosenberg LC, Goldberg HA. Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins. Biochem J. 1996;317:59–64
  215. Pampena DA, Robertson KA, Litvinova O, Lajoie G, Goldberg HA, Hunter GK. Inhibition of hydroxyapatite formation by osteopontin phosphopeptides. Biochem J. 2004;378:1083–1087
  216. Min KS, Park HJ, Lee SK, et al. Effect of mineral trioxide aggregate on dentin bridge formation and expression of dentin sialoprotein and heme oxygenase-1 in human dental pulp. J Endod. 2008;34:666–670
  217. Gomes AC, Filho JE, de Oliveira SH. MTA-induced neutrophil recruitment: a mechanism dependent on IL-1beta, MIP-2, and LTB4. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;106:450–456
  218. Brewer JM. (How) do aluminium adjuvants work?. Immunol Lett. 2006;102:10–15
  219. Rezende TM, Vieira LQ, Sobrinho AP, Oliveira RR, Taubman MA, Kawai T. The influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice. J Endod. 2008;34:1066–1071
  220. Silva MJ, Vieira LQ, Sobrinho AP. The effects of mineral trioxide aggregates on cytokine production by mouse pulp tissue. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105:e70–e76
  221. Holland R, de Souza V, Nery MJ, Otoboni Filho JA, Bernabé PF, Dezan Júnior E. Reaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggregate or calcium hydroxide. J Endod. 1999;25:161–166
  222. Moretton TR, Brown CE, Legan JJ, Kafrawy AH. Tissue reactions after subcutaneous and intraosseous implantation of mineral trioxide aggregate and ethoxybenzoic acid cement. J Biomed Mater Res. 2000;52:528–533
  223. Holland R, de Souza V, Nery MJ, et al. Reaction of rat connective tissue to implanted dentin tube filled with mineral trioxide aggregate, Portland cement or calcium hydroxide. Braz Dent J. 2001;12:3–8
  224. Holland R, de Souza V, Nery MJ, et al. Calcium salts deposition in rat connective tissue after the implantation of calcium hydroxide-containing sealers. J Endod. 2002;28:173–176
  225. Holland R, Souza V, Nery MJ, et al. Reaction of rat connective tissue to implanted dentin tubes filled with a white mineral trioxide aggregate. Braz Dent J. 2002;13:23–26
  226. Yaltirik M, Ozbas H, Bilgic B, Issever H. Reactions of connective tissue to mineral trioxide aggregate and amalgam. J Endod. 2004;30:95–99
  227. Modaresi J, Yavari SA, Dianat SO, Shahrabi S. A comparison of tissue reaction to MTA and an experimental root-end restorative material in rats. Aust Endod J. 2005;31:69–72
  228. Sumer M, Muglali M, Bodrumlu E, Guvenc T. Reactions of connective tissue to amalgam, intermediate restorative material, mineral trioxide aggregate, and mineral trioxide aggregate mixed with chlorhexidine. J Endod. 2006;32:1094–1096
  229. Shahi S, Rahimi S, Lotfi M, Yavari H, Gaderian A. A comparative study of the biocompatibility of three root-end filling materials in rat connective tissue. J Endod. 2006;32:776–780
  230. Vosoughhosseini S, Lotfi M, Shahi S, et al. Influence of white versus gray mineral trioxide aggregate on inflammatory cells. J Endod. 2008;34:715–717
  231. Prescott RS, Alsanea R, Fayad MI, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34:421–426
  232. Hwang YC, Lee SH, Hwang IN, et al. Chemical composition, radiopacity, and biocompatibility of Portland cement with bismuth oxide. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:e96–102
  233. Laliz EM, Esain ML, Kokubu GA, Willis J, Chaves C, Grana DR. Rat Subcutaneous tissue response to modified Portland cement, a new mineral trioxide aggregate. Braz Dent J. 2009;20:112–117
  234. Lotfi M, Vosoughhosseini S, Saghiri MA, Mesgariabbasi M, Ranjkesh B. Effect of white mineral trioxide aggregate mixed with disodium hydrogen phosphate on inflammatory cells. J Endod. 2009;35:703–705
  235. Gomes-Filho JE, Watanabe S, Bernabé PF, de Moraes Costa MT. A mineral trioxide aggregate sealer stimulated mineralization. J Endod. 2009;35:256–260
  236. Torabinejad M, Hong CU, Pitt Ford TR, Kaiyawasam SP. Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report. J Endod. 1995;21:569–571
  237. Torabinejad M, Ford TR, Abedi HR, Kariyawasam SP, Tang HM. Tissue reaction to implanted root-end filling materials in the tibia and mandible of guinea pigs. J Endod. 1998;24:468–471
  238. Sousa CJ, Loyola AM, Versiani MA, Biffi JC, Oliveira RP, Pascon EA. A comparative histological evaluation of the biocompatibility of materials used in apical surgery. Int Endod J. 2004;37:738–748
  239. Cintra LT, de Moraes IG, Estrada BP, et al. Evaluation of the tissue response to MTA and MBPC: microscopic analysis of implants in alveolar bone of rats. J Endod. 2006;32:556–559

PII: S0099-2399(09)00770-5

doi: 10.1016/j.joen.2009.09.010

Journal of Endodontics
Volume 36, Issue 2 , Pages 190-202 , February 2010

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