Journal of Endodontics
Volume 36, Issue 2 , Pages 264-267, February 2010

The Effect of Cultured Autologous Periodontal Ligament Cells on the Healing of Delayed Autotransplanted Dog's Teeth

  • Yixiang Wang, DDS

      Affiliations

    • Research Laboratory of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, China
    • ,
  • Gary Shun-pan Cheung, DDS, PhD

      Affiliations

    • Area of Endodontics, Faculty of Dentistry, the University of Hong Kong, HKSAR, China
    • ,
  • Xiangliang Xu, DDS

      Affiliations

    • Department of Oral Anatomy and Physiology, Peking University School and Hospital of Stomatology, Beijing, China
    • ,
  • Shijie Zhao, DDS

      Affiliations

    • Department of Oral Anatomy and Physiology, Peking University School and Hospital of Stomatology, Beijing, China
    • ,
  • Chengfei Zhang, DDS, MD

      Affiliations

    • Area of Endodontics, Faculty of Dentistry, the University of Hong Kong, HKSAR, China
    • Special Dental Department, Peking University School and Hospital of Stomatology, Beijing, China
    • Corresponding Author InformationAddress requests for reprints to Dr Chengfei Zhang, Floor 3A, Prince Philip Dental Hospital, 34 Hospital Road, Saiyingpun, Hong Kong SAR, China.

published online 28 October 2009.

Abstract 

Introduction

The regeneration of the periodontal structure for avulsed teeth extended dry times has been a goal of dentists. The aim of this study was to investigate a new strategy of delayed replantation for avulsed teeth that were not suitable for immediate replantation.

Methods

Extracted dog's premolar teeth were maintained in a dry environment for a month after isolation and proliferation of the periodontal ligament (PDL) cells. Then, tooth roots coated with 1 × 106 cultured autologous PDL cells were autotransplanted in artificial sockets created in the mandible. The dogs were sacrificed 60 days after transplantation. Histologic analyses showed that a root-PDL-bone complex was found in all cases of the PDL cell–loaded samples.

Results

The new PDL-like connective tissue was located between the alveolar bone and the transplanted roots, with fibers inserting into the newborn cementum on one end and alveolar bone on the other. For the control samples, no PDL-like tissue was found, and ankylosis was commonly observed.

Conclusions

The results indicated that cultured autologous PDL cells assist in the re-establishment of periodontal architecture of autotransplanted teeth that is devoid of viable periodontal cells.

Key Words: Delayed autotransplantation, periodontal ligament, periodontal regeneration, replantation

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 Supported by the National Natural Science Foundation of China Grant.

PII: S0099-2399(09)00774-2

doi:10.1016/j.joen.2009.09.014

Journal of Endodontics
Volume 36, Issue 2 , Pages 264-267, February 2010

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