Journal of Endodontics
Volume 36, Issue 2 , Pages 218-223 , February 2010

Polymerase Chain Reaction–Denaturing Gradient Gel Electrophoresis, Cloning, and Sequence Analysis of Bacteria Associated with Acute Periapical Abscesses in Children

  • Qiu-Bo Yang, DDS, PhD

      Affiliations

    • Beijing Institute for Dental Research, Capital Medical University School of Stomatology, Beijing, People's Republic of China
  • ,
  • Lu-Na Fan, DDS, MS

      Affiliations

    • Department of Pediatric Dentistry, Capital Medical University School of Stomatology, Beijing, People's Republic of China
  • ,
  • Qing Shi, DDS

      Affiliations

    • Department of Pediatric Dentistry, Capital Medical University School of Stomatology, Beijing, People's Republic of China
    • Corresponding Author InformationAddress requests for reprints to Dr Qing Shi, the Department of Pediatric Dentistry, Capital Medical University School of Stomatology, Tian Tan Xi Li No. 4, Chong Wen District, Beijing 100050, People's Republic of China.

References 

  1. Heimdahl A, Nord CE. Treatment of orofacial infections of odontogenic origin. Scand J Infect Dis Suppl. 1985;46:101–105
  2. Kuriyama T, Absi EG, Williams DW. An outcome audit of the treatment of acute dentoalveolar infection: impact of penicillin resistance. Br Dent J. 2005;198:759–763
  3. Peterson LJ. Principles of management and prevention of odontogenic infections. In:  Peterson LJ,  Ellis E,  Hupp JR,  Tucker MR editor. Contemporary oral and maxillofacial surgery. St Louis: MO :Mosby; 1998;p. 392–417
  4. Sandor GK, Low DE, Judd PL. Antimicrobial treatment options in the management of odontogenic infections. J Can Dent Assoc. 1998;64:508–514
  5. Munson MA, Pitt-Ford T, Chong B. Molecular and cultural analysis of the microflora associated with endodontic infections. J Dent Res. 2002;81:761–766
  6. Sakamoto M, Rôças IN, Siqueira JF. Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol. 2006;21:112–122
  7. Siqueira JF, Rôças IN, Souto R. Microbiological evaluation of acute periradicular abscesses by DNA-DNA Hybridization. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;92:451–457
  8. Lewis MA, MacFarlane TW, McGowan DA. Quantitative bacteriology of acute dento-alveolar abscesses. J Med Microbiol. 1986;21:101–104
  9. Wasfy MO, McMahon KT, Minah GE. Microbiological evaluation of periapical infections in Egypt. Oral Microbiol Immunol. 1992;7:100–105
  10. Williams BL, McCann GF, Schoenknecht FD. Bacteriology of dental abscesses of endodontic origin. J Clin Microbiol. 1983;18:770–774
  11. Sundqvist G, Johansson E, Sjögren U. Prevalence of blackpigmented Bacteroides species in root canal infections. J Endod. 1989;15:13–19
  12. Kuriyama T, Karasawa T, Nakagawa K. Bacteriologic features and antimicrobial susceptibility in isolates from orofacial odontogenic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2000;90:600–608
  13. Brook I, Frazier EH, Gher ME. Microbiology of periapical abscesses and associated maxillary sinusitis. J Periodontol. 1996;67:608–610
  14. Muyzer G, de Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol. 1993;59:695–700
  15. Siqueira JF, Rôças IN, Rosado AS. Application of denaturing gradient gel electrophoresis (DGGE) to the analysis of endodontic infections. J Endod. 2005;31:775–782
  16. Alves FR, Siqueira JF, Carmo FL. Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis. J Endod. 2009;35:486–492
  17. Siqueira JF, Rôças IN, Debelian G. Profiling of root canal bacterial communities associated with chronic apical periodontitis from Brazilian and Norwegian subjects. J Endod. 2008;34:1457–1461
  18. Li Y, Ge Y, Saxena D. Genetic profiling of the oral microbiota associated with severe early-childhood caries. J Clin Microbiol. 2007;45:81–87
  19. Torabinejad M, Walton RE. Periradicular lesions. In:  Ingle JI,  Bakland LK editor. Endodontics. Baltimore, MD: Williams and Wilkins; 1994;p. 439–464
  20. Burton JP, Cadieux PA, Reid G. Improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation. Appl Environ Microbiol. 2003;69:97–101
  21. Stackebrandt E, Goebel BM. A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol. 1994;44:846–849
  22. Aas JA, Paster BJ, Stokes LN. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 2005;42:5721–5732
  23. Rolph HJ, Lennon A, Riggio MP. Molecular identification of microorganisms from endodontic infections. J Clin Microbiol. 2001;39:3282–3289
  24. Siqueira JF, Rôças IN. Exploiting molecular methods to explore endodontic infections: part 2–redefining the endodontic microbiota. J Endod. 2005;31:488–498
  25. Siqueira JF, Rôças IN, Rosado AS. Investigation of bacterial communities associated with asymptomatic and symptomatic endodontic infections by denaturing gradient gel electrophoresis fingerprinting approach. Oral Microbiol Immunol. 2004;19:363–370
  26. Machado de Oliveira JC, Siqueira JF, Rôças IN. Bacterial community profiles of endodontic abscesses from Brazilian and USA subjects as compared by denaturing gradient gel electrophoresis analysis. Oral Microbiol Immunol. 2007;22:14–18
  27. Brook I, Frazier EH, Gher ME. Aerobic and anaerobic microbiology of acute periapical abscess. Oral Microbiol Immunol. 1991;6:123–125
  28. Stefanopoulos PK, Kolokotronis AE. The clinical significance of anaerobia bacteria in acute orofacial odontogenic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;98:398–408
  29. Riggio MP, Aga H, Murray CA. Identification of bacteria associated with spreading odontogenic infections by 16S rRNA gene sequencing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103:610–617
  30. Gomes BPFA, Montagner F, Jacinto RC. Polymerase chain reaction of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in primary endodontic infections. J Endod. 2007;33:1049–1052
  31. Sassone LM, Fidel RA, Faveri M. A microbiological profile of symptomatic teeth with primary endodontic infections. J Endod. 2008;34:541–545
  32. Foschi F, Cavrini F, Montebugnoli L. Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol. 2005;20:289–295

PII: S0099-2399(09)00944-3

doi: 10.1016/j.joen.2009.11.001

Journal of Endodontics
Volume 36, Issue 2 , Pages 218-223 , February 2010