Effect of MTAD on the Differentiation of Osteoblast-like Cells

Yasuda, Yoshiyuki; Tatematsu, Yuki; Fujii, Shinsuke; Maeda, Hidefumi; Akamine, Akifumi; Torabinejad, Mahmoud; Saito, Takashi

doi:10.1016/j.joen.2009.11.002

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Volume 36, Issue 2 , Pages 260-263, February 2010

Effect of MTAD on the Differentiation of Osteoblast-like Cells

Yoshiyuki Yasuda, DDS, PhD
- Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido
- Address requests for reprints to Dr Yoshiyuki Yasuda, Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Yuki Tatematsu, DDS
- Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido
Shinsuke Fujii, DDS, PhD
- Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
Hidefumi Maeda, DDS, PhD
- Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
Akifumi Akamine, DDS, PhD
- Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
Mahmoud Torabinejad, DMD, MSD, PhD
- Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, California
Takashi Saito, DDS, PhD
- Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido

Abstract

Introduction

The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells.

Methods

The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction.

Results

MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H₂O₂ showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 μg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH)₂ paste did not change in BSP gene expression in MC3T3-E1 cells on day 21.

Conclusions

These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H₂O₂, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine.

Key Words: Alkaline phosphatase, bone sialoprotein, cytotoxicity, differentiation, osteoblasts, periodontal ligament cells