Journal of Endodontics
Volume 36, Issue 2 , Pages 260-263 , February 2010

Effect of MTAD on the Differentiation of Osteoblast-like Cells

  • Yoshiyuki Yasuda, DDS, PhD

      Affiliations

    • Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido
    • Corresponding Author InformationAddress requests for reprints to Dr Yoshiyuki Yasuda, Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
    • ,
  • Yuki Tatematsu, DDS

      Affiliations

    • Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido
    • ,
  • Shinsuke Fujii, DDS, PhD

      Affiliations

    • Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
    • ,
  • Hidefumi Maeda, DDS, PhD

      Affiliations

    • Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
    • ,
  • Akifumi Akamine, DDS, PhD

      Affiliations

    • Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
    • ,
  • Mahmoud Torabinejad, DMD, MSD, PhD

      Affiliations

    • Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, California
    • ,
  • Takashi Saito, DDS, PhD

      Affiliations

    • Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido

References 

  1. Moller AJ, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981;89:475–484
  2. Kiryu T, Hoshino E, Iwaku M. Bacteria invading periapical cementum. J Endod. 1994;20:169–172
  3. Sen BH, Piskin B, Demirci T. Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Endod Dent Traumatol. 1995;11:6–9
  4. Jeansonne MJ, White RR. A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants. J Endod. 1994;20:276–278
  5. Bystrom A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res. 1981;89:321–328
  6. Vertucci FJ. Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol. 1984;58:589–599
  7. Torabinejad M, Handysides R, Khademi AA, Bakland LK. Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;94:658–666
  8. Sjogren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997;30:297–306
  9. Pataky L, Ivanyi I, Grigar A, Fazekas A. Antimicrobial efficacy of various root canal preparation techniques: an in vitro comparative study. J Endod. 2002;28:603–605
  10. Zehnder M. Root canal irrigants. J Endod. 2006;32:389–398
  11. Cwikla SJ, Belanger M, Giguere S, Progulske-Fox A, Vertucci FJ. Dentinal tubule disinfection using three calcium hydroxide formulations. J Endod. 2005;31:50–52
  12. Torabinejad M, Khademi AA, Babagoli J, et al. A new solution for the removal of the smear layer. J Endod. 2003;29:170–175
  13. Torabinejad M, Shabahang S, Aprecio RM, Kettering JD. The antimicrobial effect of MTAD: an in vitro investigation. J Endod. 2003;29:400–403
  14. Shabahang S, Pouresmail M, Torabinejad M. In vitro antimicrobial efficacy of MTAD and sodium hypochlorite. J Endod. 2003;29:450–452
  15. Shabahang S, Torabinejad M. Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth. J Endod. 2003;29:576–579
  16. Torabinejad M, Cho Y, Khademi AA, Bakland LK, Shabahang S. The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer. J Endod. 2003;29:233–239
  17. Krause TA, Liewehr FR, Hahn CL. The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline, and citric acid on Enterococcus faecalis. J Endod. 2007;33:28–30
  18. Davis JM, Maki J, Bahcall JK. An in vitro comparison of the antimicrobial effects of various endodontic medicaments on Enterococcus faecalis. J Endod. 2007;33:567–569
  19. Zhang W, Torabinejad M, Li Y. Evaluation of cytotoxicity of MTAD using the MTT-tetrazolium method. J Endod. 2003;29:654–657
  20. Fujii S, Maeda H, Wada N, Kano Y, Akamine A. Establishing and characterizing human periodontal ligament fibroblasts immortalized by SV40T-antigen and hTERT gene transfer. Cell Tissue Res. 2006;324:117–125
  21. Masillamoni CR, Kettering JD, Torabinejad M. The biocompatibility of some root canal medicaments and irrigants. Int Endod J. 1981;14:115–120
  22. Mjor IA. Biologic assessment of restorative dental materials: interrelationship of biologic and technologic properties. Oper Dent. 1978;3:9–13
  23. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods. 1986;89:271–277
  24. Ogata K, Imazato S, Ehara A, et al. Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites. J Biomed Mater Res. 2005;72:127–135
  25. Imazato S, Horikawa D, Ogata K, Kinomoto Y, Ebisu S. Responses of MC3T3-E1 cells to three dental resin-based restorative materials. J Biomed Mater Res. 2006;76:765–772
  26. Beck GR, Sullivan EC, Moran E, Zerler B. Relationship between alkaline phosphatase levels, osteopontin expression, and mineralization in differentiating MC3T3-E1 osteoblasts. J Cell Biochem. 1998;68:269–280
  27. Jadlowiec J, Koch H, Zhang X, Campbell PG, Seyedain M, Sfeir C. Phosphophoryn regulates the gene expression and differentiation of NIH3T3, MC3T3-E1, and human mesenchymal stem cells via the integrin/MAPK signaling pathway. J Biol Chem. 2004;279:53323–53330
  28. Marom R, Shur I, Solomon R, Benayahu D. Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells. J Cell Physiol. 2005;202:41–48
  29. Aubin JE, Liu F, Malaval L, Gupta AK. Osteoblast and chondroblast differentiation. Bone. 1995;17:77S–83S
  30. Malaval L, Wade-Gueye NM, Boudiffa M, et al. Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis. J Exp Med. 2008;205:1145–1153
  31. Leonardo MR, da Silva LA, Leonardo Rde T, Utrilla LS, Assed S. Histological evaluation of therapy using a calcium hydroxide dressing for teeth with incompletely formed apices and periapical lesions. J Endod. 1993;19:348–352

PII: S0099-2399(09)00945-5

doi: 10.1016/j.joen.2009.11.002

Journal of Endodontics
Volume 36, Issue 2 , Pages 260-263 , February 2010

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