Journal of Endodontics
Volume 36, Issue 3 , Pages 453-458 , March 2010

Side Population Increase after Simulated Transient Ischemia in Human Dental Pulp Cell

  • Jinming Wang, MS

      Affiliations

    • Department of Operative Dentistry and Endodontics, Sun Yat-sen University, Guangdong, China
  • ,
  • Xi Wei, MDS, PhD

      Affiliations

    • Department of Operative Dentistry and Endodontics, Sun Yat-sen University, Guangdong, China
  • ,
  • Junqi Ling, PhD

      Affiliations

    • Department of Operative Dentistry and Endodontics, Sun Yat-sen University, Guangdong, China
    • Corresponding Author InformationAddress requests for reprints to Dr Junqi Ling, Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China.
  • ,
  • Yijun Huang, PhD

      Affiliations

    • Department of Pharmacology, Sun Yat-sen University, Guangdong, China
  • ,
  • Qimei Gong, MS

      Affiliations

    • Department of Operative Dentistry and Endodontics, Sun Yat-sen University, Guangdong, China

References 

  1. Kim S. Regulation of pulpal blood flow. J Dent Res. 1985;64;Spec No:590–6
  2. Sloan AJ, Smith AJ. Stem cells and the dental pulp: potential roles in dentine regeneration and repair. Oral Dis. 2007;13:151–157
  3. Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000;97:13625–13630
  4. Shi S, Robey PG, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001;29:532–539
  5. Gronthos S, Brahim J, Li W, et al. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002;81:531–535
  6. Prescott RS, Alsanea R, Fayad MI, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34:421–426
  7. Huang AH, Chen YK, Chan AW, et al. Isolation and characterization of human dental pulp stem/stromal cells from nonextracted crown-fractured teeth requiring root canal therapy. J Endod. 2009;35:673–681
  8. Goodell MA, Brose K, Paradis G, et al. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med. 1996;183:1797–1806
  9. Ho MM, Ng AV, Lam S, et al. Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells. Cancer Res. 2007;67:4827–4833
  10. Wu C, Alman BA. Side population cells in human cancers. Cancer Lett. 2008;268:1–9
  11. Huls M, Russel FG, Masereeuw R. The role of ATP binding cassette transporters in tissue defense and organ regeneration. J Pharmacol Exp Ther. 2009;328:3–9
  12. Asakura A, Seale P, Girgis-Gabardo A, et al. Myogenic specification of side population cells in skeletal muscle. J Cell Biol. 2002;159:123–134
  13. McKinney-Freeman SL, Jackson KA, Camargo FD, et al. Muscle-derived hematopoietic stem cells are hematopoietic in origin. Proc Natl Acad Sci U S A. 2002;99:1341–1346
  14. Welm BE, Tepera SB, Venezia T, et al. Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Dev Biol. 2002;245:42–56
  15. Kubota H, Avarbock MR, Brinster RL. Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells. Proc Natl Acad Sci U S A. 2003;100:6487–6492
  16. Summer R, Kotton DN, Sun X, et al. Side population cells and Bcrp1 expression in lung. Am J Physiol Lung Cell Mol Physiol. 2003;285:L97–L104
  17. Iohara K, Zheng L, Ito M, et al. Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells. 2006;24:2493–2503
  18. Iohara K, Zheng L, Wake H, et al. A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells. 2008;26:2408–2418
  19. Honda MJ, Nakashima F, Satomura K, et al. Side population cells expressing ABCG2 in human adult dental pulp tissue. Int Endod J. 2007;40:949–958
  20. Reya T, Morrison SJ, Clarke MF, et al. Stem cells, cancer, and cancer stem cells. Nature. 2001;414:105–111
  21. Zhou S, Schuetz JD, Bunting KD, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001;7:1028–1034
  22. Krishnamurthy P, Schuetz JD. The ABC transporter Abcg2/Bcrp: role in hypoxia mediated survival. Biometals. 2005;18:349–358
  23. Krishnamurthy P, Ross DD, Nakanishi T, et al. The stem cell marker Bcrp/ABCG2 enhances hypoxic cell survival through interactions with heme. J Biol Chem. 2004;279:24218–24225
  24. Keith B, Simon MC. Hypoxia-inducible factors, stem cells, and cancer. Cell. 2007;129:465–472
  25. Tavaluc RT, Hart LS, Dicker DT, et al. Effects of low confluency, serum starvation and hypoxia on the side population of cancer cell lines. Cell Cycle. 2007;6:2554–2562
  26. Rafii S, Lyden D. Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med. 2003;9:702–712
  27. Gebhardt M, Murray PE, Namerow KN, et al. Cell survival within pulp and periodontal constructs. J Endod. 2009;35:63–66
  28. Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: a review of current status and a call for action. J Endod. 2007;33:377–390
  29. Yao S, Pan F, Prpic V, Wise GE. Differentiation of stem cells in the dental follicle. J Dent Res. 2008;87:767–771
  30. Kawanabe N, Murakami K, Takano-Yamamoto T. The presence of ABCG2-dependent side population cells in human periodontal ligaments. Biochem Biophys Res Commun. 2006;344:1278–1283
  31. Challen GA, Little MH. A side order of stem cells: the SP phenotype. Stem Cells. 2006;24:3–12
  32. Huls M, van den Heuvel JJ, Dijkman HB, et al. ABC transporter expression profiling after ischemic reperfusion injury in mouse kidney. Kidney Int. 2006;69:2186–2193
  33. Martin CM, Ferdous A, Gallardo T, et al. Hypoxia-inducible factor-2 alpha transactivates Abcg2 and promotes cytoprotection in cardiac side population cells. Circ Res. 2008;102:1075–1081
  34. D'Ippolito G, Diabira S, Howard GA, et al. Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells. Bone. 2006;39:513–522
  35. Grayson WL, Zhao F, Izadpanah R, et al. Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs. J Cell Physiol. 2006;207:331–339

 Supported by the National Natural Science Foundation of China (grant no. 30872876) and Natural Science Foundation of Guangdong Province (grant no. 9151008901000199), China.

PII: S0099-2399(09)01018-8

doi: 10.1016/j.joen.2009.11.018

Journal of Endodontics
Volume 36, Issue 3 , Pages 453-458 , March 2010