Journal of Endodontics
Volume 36, Issue 5 , Pages 894-898 , May 2010

Effectiveness of Chemomechanical Preparation with Alternating Use of Sodium Hypochlorite and EDTA in Eliminating Intracanal Enterococcus faecalis Biofilm

  • Janir Alves Soares, PhD

      Affiliations

    • Department of Dentistry, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
    • ,
  • Maria Auxiliadora Roque de Carvalho, MSc

      Affiliations

    • Department of Microbiology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil
    • ,
  • Suelleng Maria Cunha Santos, MSc

      Affiliations

    • Department of Dentistry, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
    • ,
  • Rafael Mangerotti Castro Mendonça

      Affiliations

    • Department of Microbiology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil
    • ,
  • Antonio Paulino Ribeiro-Sobrinho, PhD

      Affiliations

    • Department of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    • ,
  • Manoel Brito-Júnior, MSc

      Affiliations

    • Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil
    • ,
  • Paula Prazeres Magalhães, PhD

      Affiliations

    • Department of Microbiology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil
    • ,
  • Maria Helena Santos, PhD

      Affiliations

    • Department of Dentistry, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
    • ,
  • Luiz de Macêdo Farias, PhD

      Affiliations

    • Department of Microbiology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil
    • Corresponding Author InformationAddress requests for reprints to Dr Luiz de Macêdo Farias, Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, Minas Gerais, Brazil.

References 

  1. Fabricius L, Dahlén G, Sundqvist G, et al. Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci. 2006;114:278–285
  2. Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after “one-visit” endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99:231–252
  3. Soares JA, Leonardo MR, Silva LA, et al. Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes. J Appl Oral Sci. 2006;14:355–364
  4. Portenier I, Haapasalo H, Rye A, et al. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J. 2001;34:184–188
  5. Nerwich A, Figdor D, Messer HH. pH changes in root dentin over a 4-week period following root canal dressing with calcium hydroxide. J Endod. 1993;19:302–306
  6. Distel JW, Hatton JF, Gillespie MJ. Biofilm formation in medicated root canals. J Endod. 2002;28:689–693
  7. Soares JA, Pires Júnior DR. Influence of sodium hypochlorite-based irrigants on the susceptibility of intracanal microbiota to biomechanical preparation. Braz Dent J. 2006;17:310–316
  8. Molander A, Warfvinge J, Reit C, et al. Clinical and radiographic evaluation of one- and two-visit endodontic treatment of asymptomatic necrotic teeth with apical periodontitis: a randomized clinical trial. J Endod. 2007;33:1145–1148
  9. Waltimo T, Trope M, Haapasalo M, et al. Clinical efficacy of treatment procedures in endodontic infection control and one year follow-up of periapical healing. J Endod. 2005;31:863–866
  10. Siqueira JF, Rôças IN, Riche FN, et al. Clinical outcome of the endodontic treatment of teeth with apical periodontitis using an antimicrobial protocol. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;106:757–762
  11. Soares JA, Leonardo MR, Silva LA, et al. Elimination of intracanal infection in dogs' teeth with induced periapical lesions after rotary instrumentation: influence of different calcium hydroxide pastes. J Appl Oral Sci. 2006;14:172–177
  12. Siqueira JF, Rôças IN, Santos SR, et al. Efficacy of instrumentation techniques and irrigation regimes in reducing the bacterial population within root canals. J Endod. 2002;28:181–184
  13. Jiang YT, Xia WW, Liang JP, et al. Quantitative evaluation of residual endodontic microorganisms after mechanical root canal preparation different chemical preparations by real-time PCR. Shanghai Kou Qiang Yi Xue. 2009;18:10–14
  14. Zehnder M. Root canal irrigants. J Endod. 2006;32:389–398
  15. Zehnder M, Schmidlin P, Sener B, et al. Chelation in root canal therapy reconsidered. J Endod. 2005;31:817–820
  16. Surapipongpuntr P, Duangcharee W, Kwangsamai S, et al. Effect of root canal irrigants on cervical dentine permeability to hydrogen peroxide. Int Endod J. 2008;41:821–827
  17. Haapasalo M, Ørstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res. 1987;66:1375–1379
  18. Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol. 1990;6:142–149
  19. Gomes BP, Pinheiro ET, Gadê-Neto CR, et al. Microbiological examination of infected dental root canals. Oral Microbiol Immunol. 2004;19:71–76
  20. Nakajo K, Komori R, Ishikawa S, et al. Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis. Oral Microbiol Immunol. 2006;21:283–288
  21. Brundin M, Figdor D, Sundqvist G, et al. Starvation response and growth in serum of Fusobacterium nucleatum, Peptostreptococcus anaerobius, Prevotella intermedia, and Pseudoramibacter alactolyticus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:129–134
  22. Figdor D, Davies JK, Sundqvist G. Starvation survival, growth and recovery of Enterococcus faecalis in human serum. Oral Microbiol Immunol. 2003;18:234–239
  23. Kvist T, Molander A, Dahlén G, et al. Microbiological evaluation of one- and two-visit endodontic treatment of teeth with apical periodontitis: a randomized, clinical trial. J Endod. 2004;30:572–576
  24. Byström A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985;18:35–40
  25. Siqueira JF, Rôcas IN, Favieri A, et al. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod. 2000;26:331–334
  26. Siqueira JF, Lima KC, Magalhães FAC, et al. Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques. J Endod. 1999;25:332–335
  27. Ørstavik D, Kerekes K, Molven O. Effects of extensive apical reaming and calcium hydroxide dressing on bacterial infection during treatment of apical periodontitis: a pilot study. Int Endod J. 1991;24:1–7
  28. Schirrmeister JF, Liebenow AL, Braun G, et al. Detection and eradication of microorganisms in root-filled teeth associated with periradicular lesions: an in vivo study. J Endod. 2007;33:536–540
  29. Arias-Moliz MT, Ferrer-Luque CM, Espigares-García M, et al. Enterococcus faecalis biofilms eradication by root canal irrigants. J Endod. 2009;35:711–714
  30. Bryce G, O'Donnell D, Ready D, et al. Contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. J Endod. 2009;35:1243–1248
  31. Arias-Moliz MT, Ferrer-Luque CM, Espigares-Rodríguez E, et al. Bactericidal activity of phosphoric acid, citric acid, and EDTA solutions against Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;106:84–89
  32. Moreira DM, Almeida JF, Ferraz CC, et al. Structural analysis of bovine root dentin after use of different endodontics auxiliary chemical substances. J Endod. 2009;35:1023–1027
  33. Chivatxaranukul P, Dashper SG, Messer HH. Dentinal tubule invasion and adherence by Enterococcus faecalis. Int Endod J. 2008;41:873–882
  34. Kishen A, Sum CP, Mathew S, et al. Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin. J Endod. 2008;34:850–854
  35. Gu LS, Kim JR, Ling J, et al. Review of contemporary irrigant agitation techniques and devices. J Endod. 2009;35:791–804

 Supported by grants from Conselho Nacional de Pesquisa (CNPq) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG). JAS is partly supported by a postdoctoral CNPq-Brazil scholarship.

PII: S0099-2399(10)00004-X

doi: 10.1016/j.joen.2010.01.002

Journal of Endodontics
Volume 36, Issue 5 , Pages 894-898 , May 2010

Article Tools

* Image Usage & Resolution