Journal of Endodontics
Volume 36, Issue 8 , Pages 1326-1331, August 2010

The Role of Heme Oxygenase-1 in the Proliferation and Odontoblastic Differentiation of Human Dental Pulp Cells

  • Sun-Ju Kim, PhD

      Affiliations

    • Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, South Korea
    • Department of Dental Hygiene, Cheongju University, Cheongju, South Korea
    • ,
  • Kyung-San Min, DDS, PhD

      Affiliations

    • Department of Conservative Dentistry, School of Dentistry, Wonkwang University, Iksan, South Korea
    • ,
  • Hyun-Wook Ryu, DDS, MSD

      Affiliations

    • Department of Conservative Dentistry, School of Dentistry, Wonkwang University, Iksan, South Korea
    • ,
  • Hwa-Jeong Lee, PhD

      Affiliations

    • Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, South Korea
    • ,
  • Eun-Cheol Kim, DDS, PhD

      Affiliations

    • Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, South Korea
    • Corresponding Author InformationAddress requests for reprints to Dr Eun-Cheol Kim, Department of Oral and Maxillofacial Pathology, Dental College, Wonkwang University, Sinyoungdong 344-2, Iksan City, Jeonbuk, 570-749, South Korea.

published online 21 June 2010.

Abstract 

Introduction

It was recently reported that heme oxygenase-1 (HO-1) activity is related to stem cell differentiation; however, the involvement of HO-1 in pulp cell growth and differentiation has not been well explored. The purpose of this study was to investigate the role of HO-1 in the growth and differentiation of human dental pulp cells (HDPCs).

Methods

We evaluated cell growth by MTT assay, mineralization by alizarin red staining, and differentiation marker mRNA expression by reverse transcriptase polymerase chain reaction.

Results

HO-1 induction by cobaltic protoporphyrin IX (CoPP) in HDPCs increased cell growth and mineralization and up-regulated the messenger RNA expression of such odontoblastic markers as alkaline phosphatase, osteopontin, bone sialoprotein, dentin matrix protein-1, and dentin sialophosphoprotein. Carbon monoxide scavenger, iron chelator, HO-1 inhibitor, and HO-1 small interfering RNA (siRNA) attenuated HDPC growth and differentiation.

Conclusions

CoPP treatment results in dental pulp cell proliferation and odontoblast differentiation that appears partly mediated by HO-1. Our results suggest that odontoblastic differentiation and growth are positively regulated by HO-1 induction and negatively regulated by HO-1 inhibition. Thus, pharmacologic HO-1 induction might represent a potent therapeutic approach for pulp capping and the regeneration of HDPCs.

Key Words: Differentiation, growth, heme oxygenase-1, human dental pulp cells

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 This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A084458).

PII: S0099-2399(10)00383-3

doi:10.1016/j.joen.2010.04.011

Journal of Endodontics
Volume 36, Issue 8 , Pages 1326-1331, August 2010

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