Journal of Endodontics
Volume 36, Issue 8 , Pages 1326-1331 , August 2010

The Role of Heme Oxygenase-1 in the Proliferation and Odontoblastic Differentiation of Human Dental Pulp Cells

  • Sun-Ju Kim, PhD

      Affiliations

    • Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, South Korea
    • Department of Dental Hygiene, Cheongju University, Cheongju, South Korea
    • ,
  • Kyung-San Min, DDS, PhD

      Affiliations

    • Department of Conservative Dentistry, School of Dentistry, Wonkwang University, Iksan, South Korea
    • ,
  • Hyun-Wook Ryu, DDS, MSD

      Affiliations

    • Department of Conservative Dentistry, School of Dentistry, Wonkwang University, Iksan, South Korea
    • ,
  • Hwa-Jeong Lee, PhD

      Affiliations

    • Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, South Korea
    • ,
  • Eun-Cheol Kim, DDS, PhD

      Affiliations

    • Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, South Korea
    • Corresponding Author InformationAddress requests for reprints to Dr Eun-Cheol Kim, Department of Oral and Maxillofacial Pathology, Dental College, Wonkwang University, Sinyoungdong 344-2, Iksan City, Jeonbuk, 570-749, South Korea.

References 

  1. Gronthos S, Brahim J, Li W, et al. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002;81:531–553
  2. Agata H, Kagami H, Watanabe N, et al. Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells. Differentiation. 2008;76:981–993
  3. Butler WT, Ritchie H. The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol. 1995;39:169–179
  4. Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000;97:13625–13630
  5. Nakashima M. Bone morphogenetic proteins in dentin regeneration for potential use in endodontic therapy. Cytokine Growth Factor Rev. 2005;163:369–376
  6. Choi AM, Alam J. Heme oxygenase-1: function, regulation, and implication of a novel stress-inducible protein in oxidant-induced lung injury. Am J Respir Cell Mol Biol. 1996;15:9–19
  7. Lee SK, Choi HI, Yang YS, et al. Nitric oxide modulates osteoblastic differentiation with heme oxygenase-1 via the mitogen activated protein kinase and nuclear factor-kappaB pathways in human periodontal ligament cells. Biol Pharm Bull. 2009;32:1328–1334
  8. Min KS, Hwang YH, Ju HJ, et al. Heme oxygenase-1 mediates cytoprotection against nitric oxide-induced cytotoxicity via the cGMP pathway in human pulp cells. Oral Surg Oal Med Oral Pathol Oral Radol Endod. 2006;102:803–808
  9. Min KS, Kwon YY, Lee HJ, et al. Effects of proinflammatory cytokines on the expression of mineralization markers and heme oxygenase-1 in human pulp cells. J Endod. 2006;32:39–43
  10. Pi SH, Kim SC, Kim HT, et al. Defense of heme oxygenase-1 against cytotoxic and receptor of NF kappa-B ligand inducing effects of hydrogenin human periodontal ligament cells. J Periodontal Res. 2007;42:331–339
  11. Lee SK, Pi SH, Kim SH, et al. Substance P regulates macrophage inflammatory protein 3α/CCL20 with hemein human periodontal ligament cells. Clin Exp Immunol. 2007;150:567–575
  12. Kook YA, Lee SK, Son DH, et al. Effects of substance P on osteoblastic differentiation and heme oxygenase-1 in human periodontal ligament cells. Cell Biol Int. 2009;33:424–428
  13. Kim DH, Burgess AP, Li M, et al. Heme oxygenase-mediated increases in adiponectin decrease fat content and inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 in Zucker rats and reduce adipogenesis in human mesenchymal stem cells. J Pharmacol Exp Ther. 2008;325:833–840
  14. Barbagallo I, Tibullo D, Di Rosa M, et al. A cytoprotective role for the heme oxygenase-1/CO pathway during neural differentiation of human mesenchymal stem cells. J Neurosci Res. 2008;86:1927–1935
  15. Johns DG, Zelent D, Ao Z, et al. Heme-oxygenase induction inhibits arteriolar thrombosis in vivo: effect of the non-substrate inducer cobalt protoporphyrin. Eur J Pharmacol. 2009;606:109–114
  16. Wang JY, Lee YT, Chang PF, et al. Hemin promotes proliferation and differentiation of endothelial progenitor cells via activation of AKT and ERK. J Cell Physiol. 2009;219:617–625
  17. Chou CC, Hsu CY. Involvement of PKC in TPA-potentiated apoptosis induction during hemin-mediated erythroid differentiation in K562 cells. Naunyn Schmiedebergs Arch Pharmacol. 2009;379:1–9
  18. Kitagawa M, Ueda H, Iizuka S, et al. Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. Arch Oral Biol. 2007;52:727–731
  19. Yasuda Y, Ogawa M, Arakawa T, et al. The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study. J Endod. 2008;34:1057–1060
  20. Min KS, Lee YM, Hong SO, et al. Simvastatin promotes odontoblastic differentiation and expression of angiogenic factors via heme oxygenase-1 in primary cultured human dental pulp cells. J Endod. 2010;36:447–452
  21. Min KS, Yang SH, Kim EC. The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells. J Endod. 2009;35:847–851
  22. Shi S, Bartold PM, Miura M, et al. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res. 2005;8:191–199
  23. Lin HY, Shen SC, Lin CW, et al. Cobalt protoporphyrin inhibition of lipopolysaccharide or lipoteichoic acid-induced nitric oxide production via blocking c-Jun N-terminal kinase activation and nitric oxide enzyme activity. Chem Biol Interact. 2009;180:202–210
  24. Listopad J, Asadullah K, Sievers C, et al. Heme oxygenase-1 inhibits T cell-dependent skin inflammation and differentiation and function of antigen-presenting cells. Exp Dermatol. 2007;16:661–670
  25. Vanella L, Kim DH, Asprinio D, et al. HO-1 expression increases mesenchymal stem cell-derived osteoblast but decreases adipocyte lineage. Bone. 2010;46:236–243
  26. Ward RJ, Kuhn LC, Kaldy P, et al. Control of cellular iron homeostasis by iron-responsive elements in vivo. Eur J Biochem. 1994;220:927–931
  27. Verma A, Hirsch DJ, Glatt CE, et al. Carbon monoxide: a putative neural messenger. Science. 1993;259:381–384
  28. Otterbein LE, Bach FH, Alam J, et al. Carbon monoxide has anti-inflammatory effects involving the mitogen-activated protein kinase pathway. Nat Med. 2000;6:422–428
  29. Raser JM, O'Shea EK. Noise in gene expression: origins, consequences, and control. Science. 2005;309:2010–2013

 This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A084458).

PII: S0099-2399(10)00383-3

doi: 10.1016/j.joen.2010.04.011

Journal of Endodontics
Volume 36, Issue 8 , Pages 1326-1331 , August 2010

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