Journal of Endodontics
Volume 36, Issue 9 , Pages 1475-1479 , September 2010

Molecular Fingerprinting Reveals the Presence of Unique Communities Associated with Paired Samples of Root Canals and Acute Apical Abscesses

  • Francisco Montagner, DDS, MSc, PhD

      Affiliations

    • Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil
    • ,
  • Brenda P.F.A. Gomes, DDS, MSc, PhD

      Affiliations

    • Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil
    • Corresponding Author InformationAddress requests for reprints to Dr Brenda P.F.A. Gomes, Piracicaba Dental School, University of Campinas, Endodontic Division, Av Limeira 901, Bairro Areiao, Piracicaba, São Paulo, Brasil CEP 13414-903.
    • ,
  • Purnima S. Kumar, DDS, PhD

      Affiliations

    • Division of Periodontology, College of Dentistry, The Ohio State University, Columbus, OH, USA

References 

  1. Boykin MJ, Gilbert GH, Tilashalski KR, et al. Incidence of endodontic treatment: a 48-month prospective study. J Endod. 2003;29:806–809
  2. Walton RE, Torabinejad M. Principles and Practice of Endodontics. 3rd ed.. Philadelphia: Saunders; 2002;
  3. Flynn TR, Shanti RM, Levi MH, et al. Severe odontogenic infections, part 1: prospective report. J Oral Maxillofac Surg. 2006;64:1093–1103
  4. De Sousa EL, Ferraz CC, Gomes BP, et al. Bacteriological study of root canals associated with periapical abscesses. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:332–339
  5. Rôças IN, Baumgartner JC, Xia T, et al. Prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. J Endod. 2006;32:1135–1138
  6. Sakamoto M, Rôças IN, Siqueira JF, et al. Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol. 2006;21:112–122
  7. Siqueira JF, Rôças IN. Exploiting molecular methods to explore endodontic infections: part 1—current molecular technologies for microbiological diagnosis. J Endod. 2005;31:411–423
  8. Siqueira JF, Rôças IN. Diversity of endodontic microbiota revisited. J Dent Res. 2009;88:969–981
  9. Siqueira JF, Rôças IN, Souto R, et al. Microbiological evaluation of acute periradicular abscesses by DNA-DNA hybridization. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;92:451–457
  10. Siqueira JF, Rôças IN. The microbiota of acute apical abscesses. J Dent Res. 2009;88:61–65
  11. Machado de Oliveira JC, Siqueira JF, Rôças IN, et al. Bacterial community profiles of endodontic abscesses from Brazil and USA subjects as compared by denaturing gradient gel electrophoresis analysis. Oral Microbiol Immunol. 2007;22:14–18
  12. Saito D, Marsh TL, de Souza Cannavan F, et al. Assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis. Oral Microbiol Immunol. 2009;24:369–376
  13. Munson MA, Pitt-Ford T, Chong B, et al. Molecular and cultural analysis of the microflora associated with endodontic infections. J Dent Res. 2002;81:761–766
  14. Jacinto RC, Gomes BP, Desai M, et al. Bacterial examintaion of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. Oral Microbiol Immunol. 2007;22:403–410
  15. Riggio MP, Aga H, Murray CA, et al. Identification of bacteria associated with spreading odontogenic infections by 16S rRNA gene sequencing. Oral Sur Oral Med Oral Pathol Oral Radiol Endod. 2007;103:610–617
  16. Marsh TL. Terminal restriction fragment length polymorphism (t-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products. Curr Opin Microbiol. 1999;2:323–327
  17. Osborn AM, Moore ERB, Timmis KN. An evaluation of terminal-restriction fragment length polymorphism (t-RFLP) analysis for the study of microbial community structure and dynamics. Environ Microbiol. 2000;2:39–50
  18. Schütte UME, Abdo Z, Bent SJ, et al. Advances in the use of terminal restriction fragment length polymorphism (t-RFLP) analysis of 16S rRNA genes to characterize microbial communities. Appl Microbiol Biotechnol. 2008;80:365–380
  19. Fullmer SC, Preshaw PM, Heasman PA, et al. Smoking cessation alters subgingival microbial recolonization. J Dent Res. 2009;88:524–528
  20. Gomes BP, Pinheiro ET, Gadê-Neto CR, et al. Microbiological examination of infected dental root canals. Oral Microbiol Immunol. 2004;19:71–76
  21. Rees GN, Baldwin SK, Watson GO, et al. Ordination and significance testing of microbial community composition derived from terminal restriction fragment length polymorphisms: application of multivariate statistics. Antonie Van Leeuwenhoek. 2004;86:339–347
  22. Hommez GMG, Verhelst R, Vaneechoutte M, et al. Terminal restriction fragment length polymorphism analysis of the microflora in necrotic teeth of patients irradiated in the head and neck region. J Endod. 2008;34:1048–1051
  23. Avaniss-Aghajani E, Jones K, Holtzman A, et al. Molecular technique for rapid identification of mycobacteria. J Clin Microbiol. 1996;34:98–102
  24. Dymock D, Weightman AJ, Scully C, et al. Molecular analysis of microflora associated with dentoalveolar abcesses. J Clin Microbiol. 1996;34:537–542
  25. Siqueira JF, Rôças IN, Debelian GJ, et al. Profiling root canal bacterial communities associated with chronic apical periodontitis from Brazilian and Norwegian subjects. J Endod. 2008;34:1457–1461
  26. Alves FR, Siqueira JF, Carmo FL, et al. Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis. J Endod. 2009;35:486–492
  27. Siqueira JF, Rôças IN. Community as the unit of pathogenicity: an emerging concept as to the microbial pathogenesis of apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:870–878
  28. Grenier D, Mayrand D. Nutritional relationships between oral bacteria. Infect Immun. 1986;53:616–620
  29. Subramanian K, Mickel AK. Molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile. J Endod. 2009;35:950–957
  30. Krall EA, Abreu Sosa C, Garcia C, et al. Cigarette smoking increases the risk of root canal treatment. J Dent Res. 2006;85:313–317
  31. Okayama H, Nagata E, Ito HO, et al. Experimental abscess formation caused by human dental plaque. Microbiol Immunol. 2005;49:399–405
  32. Van Winkelhoff AJ, Bosch-Tijhof CJ, Winkel EG, et al. Smoking affects the subgingival microflora in periodontitis. J Periodontol. 2001;72:666–671
  33. Zambon JJ, Grossi SG, Machtei EE, et al. Cigarette smoking increases the risk for subgingival infection with periodontal pathogens. J Periodontol. 1996;67:1050–1054
  34. Sasagawa S, Suzuki K, Sakatani T, et al. Effect of nicotine on the functions of human polymorphonuclear leukocytes in vitro. J Leukoc Biol. 1985;37:493–502
  35. Gomes BP, Lilley JD, Drucker DB. Associations of endodontic symptoms and signs with particular combinations of specific bacteria. Int Endod J. 1996;29:69–75

 Supported by a seed grant from the College of Dentistry, The Ohio State University to Dr Purnima Kumar and the Brazilian Agencies FAPESP (2007/58518-4, 2008/56425-1, 2008/06162-4; 2008/57551-0) and CNPq (305437/2006-2, 470820/2006-3, 471631/2008-6; 302575/2009-0).

PII: S0099-2399(10)00507-8

doi: 10.1016/j.joen.2010.06.004

Journal of Endodontics
Volume 36, Issue 9 , Pages 1475-1479 , September 2010

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