Journal of Endodontics
Volume 36, Issue 10 , Pages 1617-1621 , October 2010

Apical Root Canal Microbiota as Determined by Reverse-capture Checkerboard Analysis of Cryogenically Ground Root Samples from Teeth with Apical Periodontitis

  • Isabela N. Rôças, PhD

      Affiliations

    • Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, RJ
    • ,
  • Flávio R.F. Alves, PhD

      Affiliations

    • Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, RJ
    • ,
  • Adriana L. Santos, MSc

      Affiliations

    • Institute of Microbiology Prof Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
    • ,
  • Alexandre S. Rosado, PhD

      Affiliations

    • Institute of Microbiology Prof Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
    • ,
  • José F. Siqueira Jr., PhD

      Affiliations

    • Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, RJ
    • Corresponding Author InformationAddress requests for reprints to José F. Siqueira Jr, DDS, MSc, PhD, Faculty of Dentistry, Estácio de Sá University, Av. Alfredo Baltazar da Silveira, 580/cobertura, Recreio Rio de Janeiro, RJ, Brazil 22790-710.

References 

  1. Baumgartner JC, Hutter JW, Siqueira JF. Endodontic microbiology and treatment of infections. In:  Cohen S,  Hargreaves KM editor. Pathways of the pulp. 9th ed. St Louis: Mosby/Elsevier; 2006;p. 580–607
  2. Siqueira JF. Reaction of periradicular tissues to root canal treatment: benefits and drawbacks. Endod Topics. 2005;10:123–147
  3. Sathorn C, Parashos P, Messer HH. How useful is root canal culturing in predicting treatment outcome?. J Endod. 2007;33:220–225
  4. Siqueira JF, Rôças IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97:85–94
  5. Alves FR, Siqueira JF, Carmo FL, et al. Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis. J Endod. 2009;35:486–492
  6. Siqueira JF, Rôças IN. Molecular analysis of endodontic infections. In:  Fouad AF editors. Endodontic microbiology. Ames, Iowa: Wiley-Blackwell; 2009;p. 68–107
  7. Spratt DA. Significance of bacterial identification by molecular biology methods. Endod Topics. 2004;9:5–14
  8. Baumgartner JC. Microbiological and molecular analysis of endodontic infections. Endod Topics. 2004;7:35–51
  9. Siqueira JF, Rôças IN. Exploiting molecular methods to explore endodontic infections: part 2—redefining the endodontic microbiota. J Endod. 2005;31:488–498
  10. Siqueira JF, Rôças IN. Diversity of endodontic microbiota revisited. J Dent Res. 2009;88:969–981
  11. Simon JH. The apex: how critical is it?. Gen Dent. 1994;42:330–334
  12. Ricucci D, Siqueira JF, Bate AL, Pitt Ford TR. Histologic investigation of root canal-treated teeth with apical periodontitis: a retrospective study from twenty-four patients. J Endod. 2009;35:493–502
  13. Miller WD. An introduction to the study of the bacterio-pathology of the dental pulp. Dent Cosmos. 1894;36:505–528
  14. Thilo BE, Baehni P, Holz J. Dark-field observation of the bacterial distribution in root canals following pulp necrosis. J Endod. 1986;12:202–205
  15. Fabricius L, Dahlén G, Ohman AE, Möller AJR. Predominant indigenous oral bacteria isolated from infected root canals after varied times of closure. Scand J Dent Res. 1982;90:134–144
  16. Baumgartner JC, Falkler WA. Bacteria in the apical 5 mm of infected root canals. J Endod. 1991;17:380–383
  17. Dougherty WJ, Bae KS, Watkins BJ, Baumgartner JC. Black-pigmented bacteria in coronal and apical segments of infected root canals. J Endod. 1998;24:356–358
  18. Siqueira JF, Rôças IN, Alves FR, Silva MG. Bacteria in the apical root canal of teeth with primary apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:721–726
  19. Siqueira JF, Rôças IN, Alves FR, Santos KR. Selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. J Endod. 2004;30:638–643
  20. Siqueira JF, Rôças IN. Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. J Clin Microbiol. 2005;43:3314–3319
  21. Rôças IN, Siqueira JF. Root canal microbiota of teeth with chronic apical periodontitis. J Clin Microbiol. 2008;46:3599–3606
  22. Siqueira JF, Rôças IN. The microbiota of acute apical abscesses. J Dent Res. 2009;88:61–65
  23. Rôças IN, Hulsmann M, Siqueira JF. Microorganisms in root canal-treated teeth from a German population. J Endod. 2008;34:926–931
  24. Sweet D, Hildebrand D. Recovery of DNA from human teeth by cryogenic grinding. J Forensic Sci. 1998;43:1199–1202
  25. Lijnen I, Willems G. DNA research in forensic dentistry. Methods Find Exp Clin Pharmacol. 2001;23:511–517
  26. Brito LC, Teles FR, Teles RP, et al. Use of multiple-displacement amplification and checkerboard DNA-DNA hybridization to examine the microbiota of endodontic infections. J Clin Microbiol. 2007;45:3039–3049
  27. Lovmar L, Syvanen AC. Multiple displacement amplification to create a long-lasting source of DNA for genetic studies. Hum Mutat. 2006;27:603–614
  28. Lasken RS. Genomic DNA amplification by the multiple displacement amplification (MDA) method. Biochem Soc Trans. 2009;37:450–453
  29. Gonzalez JM, Portillo MC, Saiz-Jimenez C. Multiple displacement amplification as a pre-polymerase chain reaction (pre-PCR) to process difficult to amplify samples and low copy number sequences from natural environments. Environ Microbiol. 2005;7:1024–1028
  30. Grover A, Azmi W, Paul Khurana SM, Chakrabarti SK. Multiple displacement amplification as a pre-polymerase chain reaction (pre-PCR) to detect ultra low population of Ralstonia solanacearum (Smith 1896) Yabuchi et al (1996). Lett Appl Microbiol. 2009;49:539–543
  31. Figdor D, Sundqvist G. A big role for the very small: understanding the endodontic microbial flora. Aust Dent J. 2007;52:S38–S51
  32. Siqueira JF. Microbiology of apical periodontitis. In:  Ørstavik D,  Pitt Ford T editor. Essential endodontology. 2nd ed. Oxford, UK: Blackwell Munksgaard Ltd; 2008;p. 135–196
  33. Rôças IN, Siqueira JF. Identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach. J Endod. 2010;36:45–52
  34. Rôças IN, Siqueira JF. Prevalence of new candidate pathogens Prevotella baroniae, Prevotella multisaccharivorax and as-yet-uncultivated Bacteroidetes clone X083 in primary endodontic infections. J Endod. 2009;35:1359–1362
  35. Siqueira JF. Endodontic infections: concepts, paradigms, and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;94:281–293

PII: S0099-2399(10)00564-9

doi: 10.1016/j.joen.2010.07.001

Journal of Endodontics
Volume 36, Issue 10 , Pages 1617-1621 , October 2010

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