Journal of Endodontics
Volume 14, Issue 7 , Pages 363-371 , 1988

Endodontic microbiology. I. Etiology

  • Paul A. Farber, DDS, PhD

      Affiliations

    • Dr. Farber is professor of pathology, Temple University School of Medicine, Philadelphia, PA. Dr. Seltzer is Professor Emeritus, Department of Endodontology, Temple University School of Dentistry.
  • ,
  • Samuel Seltzer, DDS

      Affiliations

    • Dr. Farber is professor of pathology, Temple University School of Medicine, Philadelphia, PA. Dr. Seltzer is Professor Emeritus, Department of Endodontology, Temple University School of Dentistry.

References 

  1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures on dental pulps in germ-free and conventional laboratory rats. Oral Surg. 1965;20:340–349
  2. Brown LR, Rudolph CE. Isolation and identification of microorganisms from unexposed canals of pulp-involved teeth. Oral Surg. 1957;10:1094–1099
  3. Leavitt JM, Naidorf IJ, Shugaevsky P. The bacterial flora of root canals as disclosed by a culture medium for endodontics. Oral Surg. 1958;11:302–308
  4. MacDonald JB, Hare GC, Wood AWS. The bacteriologic status of the pulp chambers in intact teeth found to be non-vital following trauma. Oral Surg. 1957;10:318–322
  5. Crawford JJ, Shankle RJ. Application of newer methods to study the importance of root canal and oral microbiota in endodontics. Oral Surg. 1961;14:1109–1123
  6. Serene TP, Anderson DL. Isolation of mycoplasma from human root canals. J Dent Res. 1967;46:395–399
  7. Rauch B. Attempts to isolate a virus from infected root canals and periapical tissues using monkey cell cultures. J Can Dent Assoc. 1958;24:404–410
  8. Shindell E. Studies on the possible presence of a virus in subacute and chronic periapical granulomas. Oral Surg. 1962;15:1382–1384
  9. Sundqvist GK, Eckerborn MI, Sjogren UT. Capacity of anaerobic bacteria from necrotic pulps to induce purulent infections. Infect Immun. 1979;25:685–693
  10. Sundqvist G, Bloom GD, Enberg K, Johansson E. Phagocytosis of Bacteroides melaninogenicus and Bacteroides gingivalis in vitro by human neutrophils. J Periodont Res. 1982;17:113–121
  11. Dahlén G, Hofstad T. Endotoxic activities in lipopolysaccharides of microorganisms isolated from an infected root canal in Macaca cynomolgus. Scand J Dent Res. 1977;85:272–278
  12. Dahlén G, Bergenholtz G. Endotoxic activity in teeth with necrotic pulps. J Dent Res. 1980;59:1033–1040
  13. Griffee MB, Patterson SS, Miller CH, Kafrawy AH, de Obarrio JJ. Bacteriodes melaninogenicus and dental infections. Some questions and some answers. Oral Surg. 1982;54:486–489
  14. Morris JG. Oxygen and growth of the oral bacteria. In: Kleinberg I, Ellison SA, Mandel ID editor. Proceedings: saliva and dental caries. Special Supplement Microbiol Abstract. 1979;p. 293–306
  15. Tanner ACR, Haffer C, Bratthall GT, Visconti RA, Socransky SS. A study of the bacteria associated with advancing periodontitis in man. J Clin Periodontol. 1979;6:278–307
  16. Spiegel CA, Hayduk SE, Minah GE, Krywolap GN. Black-pigmented bacteroides from clinically-characterized periodont sites. J Periodont Res. 1979;14:376–382
  17. Talley FP. Therapeutic approaches to anaerobic infection. Hosp Pract. 1981;16:117–132
  18. Berg J-O, Nord C-E. A method for isolation of anaerobic bacteria for endodontic specimens. Scand J Dent Res. 1973;81:163–166
  19. Crowley M. The culture medium in relation to endodontic culturing. In: Grossman LI editors. Trans Third International Conference on Endodontics. Philadelphia. 1963;p. 72–79
  20. Griffee MB, Patterson SS, Miller CH, Kafrawy AH, Newton CW. Comparison of bacterial growth in an improperly but commonly used medium versus reduced thioglycollate with the use of an anaerobic sampling technique. Oral Surg. 1981;52:433–436
  21. Gorbach SL, Bartlett JG. Anaerobic infections. N Engl J Med. 1977;290:1111–1124
  22. Fulghum RS, Wiggins CB, Mullaney TP. Pilot study for detecting obligate anaerobic bacteria in necrotic dental pulps. J Dent Res. 1973;52:637
  23. Holdeman LV, Cato E, Moore W. Anaerobic laboratory manual. Blacksburg, VA: Virginia Polytechnic Institute; 1977;
  24. Engström B, Frostell G. Bacteriological studies of the nonvital pulp in cases with intact pulp cavities. Acta Odontol Scand. 1961;19:23–39
  25. Wittgow WC, Sabiston CB. Microorganisms from pulpal chambers of infact teeth with necrotic pulps. J Endodon. 1975;1:168–171
  26. Kendell K, Conte M, Fujimoto L, Ernest M, Berry HG. Microorganisms isolated from pulp chambers. J Endodon. 1976;2:146–148
  27. Carlsson J, Frolander F, Sundqvist G. Oxygen tolerance of anaerobic bacteria isolated from necrotic dental pulps. Acta Odontol Scand. 1977;35:139–145
  28. Byström A, Sundqvist G. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg. 1983;55:307–312
  29. Zavistoski J, Dzink J, Oderdonk A, Bartlett J. Quantitative bacteriology of endodontic infections. Oral Surg. 1980;49:171–174
  30. Hampp EG. Isolation and identification of spirochetes obtained from unexposed canals of pulp-involved teeth. Oral Surg. 1957;10:1100–1104
  31. Loesche WJ, Gusberti F, Mettraux G, Higgins T, Syed S. Relationship between oxygen tension and subgingival bacterial flora in untreated human periodontal pockets. Infect Immun. 1983;12:659–667
  32. Kantz WE, Henry CA. Isolation and classification of anaerobic bacteria from intact pulp chambers of non-vital teeth in man. Arch Oral Biol. 1974;19:91–96
  33. Trope M, Rosenberg ES, Tronstad L. Darkfield microscopy as a diagnostic aid in differentiating endodontic and periodontic abscesses [Abstract 551]. J Dent Res. 1986;65:230
  34. Hobson P. An investigation into the bacteriologic control of infected root canals. Br Dent J. 1959;106:63–70
  35. Melville TH, Slack GL. Bacteria isolated from root canals during endodontic treatment. Br Dent J. 1961;110:127–130
  36. van Amerongen J, Slaterus WJ, Eggink CO. De toepassing van bacteriologische controles in de endodontie. Tschr Tandheelk. 1957;64:495–519
  37. Eggink CO. The value of the bacteriological culture in endodontics. I. The influence of infection during and after treatment. Int Endod J. 1982;15:79–86
  38. Stobberingh EE, Eggink CO. The value of the bacteriological culture in endodontics. II. The bacteriological flora of endodontic specimens. Int Endod J. 1982;15:87–93
  39. Möller AJR, Fabricius L, Dahlén G, Öhman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981;89:475–484
  40. Elin R, Wolff S. Biology of endotoxin. Ann Rev Immunol. 1984;27:127–141
  41. Joiner KA, Brown EJ, Frank MM. Complement and bacteria: chemistry and biology in host defense. Ann Rev Immunol. 1984;2:461–491
  42. Kimball HR, Melman KL, Wolff SM. Endotoxin-induced kinin production in man. Proc Soc Exp Biol Med. 1972;139:1078–1082
  43. Shen SM-C, Rappaport SI, Feinstein DI. Intravascular clotting after endotoxin in rabbits with impaired intrinsic clotting produced by a factor VIII antibody. Blood. 1973;42:523–534
  44. Hook WA, Snyderman R, Mergenhagen SE. Histamine releasing factor generated by the interaction of endotoxin with hamster serum. Infect Immun. 1970;2:462–467
  45. Mergenhagen SE. Complement as a mediator of inflammation. Formation of biologically-active products after interaction of serum complement with endotoxins and antigen-antibody complexes. J Periodontol. 1970;41:202–204
  46. Beutler B, Minsark IW, Cerami AC. Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin. Science. 1985;229:869–871
  47. Durum SK, Schmidt JA, Oppenheim JJ. Interleukin 1: an immunological perspective. Ann Rev Immunol. 1985;3:263–287
  48. Worthen GS, Haslett C, Smedly LA, et al. Lung vascular injury induced by chemotactic factors: enhancement by bacterial endotoxins. Fed Proc. 1986;45:7–12
  49. Rosentreich DL, Nowotny A, Chused T, Mergenhagen SE. In vitro transformation of mouse bone marrow-derived (B) lymphocytes. Infect Immun. 1973;8:406–411
  50. Hausmann E, Raisz LG, Miller RA. Endotoxin stimulation of bone resorption in tissue culture. Science. 1970;168:862–864
  51. Aleo JJ, De Renzis FA, Farber PA, Varboncoeur AP. The presence and biologic activity of cementum-bound endotoxin. J Periodontol. 1974;45:672–675
  52. Aleo JJ, De Renzis FA, Farber PA. In vitro attachment of human gingival fibroblasts to root surfaces. J Periodontol. 1975;46:639–645
  53. Fabricius L, Dahlén G, Öhman AE, Möller AJR. Predominant indigenous oral bacteria isolated from infected root canals after varied times of closure. Scand J Dent Res. 1982;9:134–144
  54. Schein B, Schilder H. Endotoxin content in endodontically involved teeth. J Endodon. 1975;1:19–21
  55. Stark A, Ten Cate JW. Endotoxin testing revisited. Eur J Clin Microbiol. 1985;4:382–385
  56. Dwyer TG, Torabinejad M. Radiographic and histologic evaluation of the effect of endotoxin on the periapical tissues of the cat. J Endodon. 1981;7:31–35
  57. Pitts DL, Williams BL, Norton TH. Investigation of the role of endotoxin inflammation. J Endodon. 1982;8:10–18
  58. Dahlén G, Magnusson BC, Möller A. Histological and histochemical study of the influence of lipopolysaccharide extracted from Fusobacterium nucleatum on the periapical tissues of the monkey, Macaca fascicularis. Arch Oral Biol. 1981;26:591–598
  59. Warfvinge J, Dahlén G, Bergenholtz G. Dental pulp response to bacterial cell wall material. J Dent Res. 1985;64:1046–1050
  60. Nowotny A, Sanavi F. Induction of nonspecific tolerance to endotoxins reduces the alveolar bone resorption in ligature-treated rats. Infect Immun. 1983;39:873–878
  61. Wesselink PR, Thoden van Velzen SK, Makkes PCh. Release of endotoxin in an experimental model simulating the dental root canal. Oral Surg. 1978;45:789–795
  62. Pinero GJ, Kiatpongsah S, Hutchins MO, Hoover J. The effect of endotoxin on the synthesis of connective tissue matrix components by pulp fibroblasts in vitro. J Endodon. 1983;9:2–6
  63. Housmann E, Kaufman E. Collagenase activity in a particulate fraction from Bacteroldes melaninogenicus. Biochem Biophys Acta. 1969;194:612–615
  64. Weiss C. The pathogenicity of Bacteroides melaninogenicus and its importance in surgical infections. Surgery. 1943;13:683–691
  65. Miller RL, Webster ME, Melmon KL. Interaction of leukocytes and endotoxin with the plasmin and kinin systems. Eur J Pharmacol. 1975;33:53–60
  66. Penner A, Bernheim AI. Studies in the pathogenesis of experimental dysentery intoxication. Production of lesions by introduction of toxin into cerebral ventricles. J Exp Med. 1960;111:145–153
  67. Palmerio C, Ming SC, Frank E, Fine J. The role of the sympathetic nervous system in the generalized Schwartzman reaction. J Exp Med. 1962;115:609–612
  68. Alper M, Palmerio C, Fine J. A further note on the mechanism of endotoxin. Proc Soc Exp Biol Med. 1967;124:537–538
  69. Parnas I, Reinhold R, Fine J. Synaptic transmission in the crayfish: increased release of transmitter substance by bacterial endotoxin. Science. 1971;171:1153–1155
  70. Schonfeld SE, Greening AB, Glick DH, Frank AL, Simon JH, Herles SM. Endotoxic activity in periapical lesions. Oral Surg. 1982;53:82–87
  71. Sundqvist G. Bacteriological studies of necrotic dental pulps. [PhD Thesis]. Umea University Odontol Dissertation no. 7. 1976;1–94
  72. Griffee MB, Patterson SS, Miller CH, Kafrawy AH, Newton CW. The relationship of Bacteroides melaninogenicus to symptoms associated with pulpal necrosis. Oral Surg. 1980;50:457–461
  73. Panopoulos P. Intradental sensory nerve responses to some factors affecting dentin and pulp. In: Thesis. Stockholm: Karolinska Institute; 1983;
  74. Panopoulos P, Mejare B, Edwall L. Effects of ammonia and organic acids on the intradental sensory nerve activity. Acta Odontol Scand. 1983;41:209–215
  75. Shockman GD, Barrett JF. Structure, function and assembly of Gram positive bacteria. Ann Rev Microbiol. 1983;37:501–527
  76. Cromartie WJ, Craddock JG, Schwab JH, Anderle S, Yang CH. Arthritis in rats after systematic injection of streptococcal cells or cell walls of Gram positive bacteria. J Exp Med. 1977;146:1585–1602
  77. Koga T, Kakimoto K, Hirofuji Kotani S, et al. Acute joint inflammation after systemic injection of the cell wall, its peptidoglycan and chemically defined peptidoglycan subunits from various bacteria. Infect Immun. 1985;50:27–34
  78. Page RC, Davies P, Allison AC. Pathogenesis of the chronic inflammatory lesion induced by group A streptococcal cell walls. Lab Invest. 1974;30:568–581
  79. Allen JB, Malone DG, Wahl SM, Calandra GB, Wilder RL. Role of the thymus in streptococcal cell wall-induced arthritis and hepatic granuloma formation. J Clin Invest. 1985;76:1042–1056
  80. Baker JJ, Billy SA. Activation of the alternate complement pathway by peptidoglycan of Actinomyces viscosus, a potentially pathogenic oral bacterium. Arch Oral Biol. 1983;28:1073–1075
  81. Hunter N. The interaction of bacterial peptidoglycan with macrophages in chronic inflammation. J Dent Res. 1984;63:427–430
  82. Wicken AJ, Knox KW. Chemical composition and properties of amphiphiles. In: Shockman GD, Wicken AJ editor. Chemistry and biological activities of bacterial surface amphiphiles. New York: Academic Press; 1981;p. 1–9
  83. Meikle MC, Gowen M, Reynolds JJ. Effect of streptococcal cell wall components on bone cell metabolism in vitro. Calcif Tissue Int. 1982;34:359–364
  84. Fiedel BA, Jackson RW. Complement activation by a streptococcal LTA. In: Shockman GD, Wicken AJ editor. Chemistry and biological activities of bacterial surface amphiphiles. New York: Academic Press; 1981;
  85. Stabholz A, Sela MN. The role of microorganisms in the pathogenesis of periapical pathosis. I. Effects of Streptococcus mutans and its cellular constituents on the dental pulp and periapical tissue of cats. J Endodon. 1983;9:171–175
  86. Korzen BH, Krakow AA, Green DB. Pulpal and periapical tissue responses in conventional and monoinfected gnotobiotic rats. Oral Surg. 1974;37:783–802
  87. Rosengren L, Winblad B. Periapical destructions caused by experimental pulpal inoculation of Streptococcus mutans in rats. Oral Surg. 1975;39:479–487
  88. Bergenholtz G, Warfvinge J. Migration of leukocytes in dental pulp in response to plaque bacteria. Scand J Dent Res. 1982;90:354–362
  89. Farber PA, Glasgow LA. Effect of Corynebacterium acnes on interferon production in mice. Infect Immun. 1972;6:272–276
  90. Webster GF, Leyden JT. Characterization of serum polymorphonuclear leukocyte chemotactic factors produced by Propionibacterium acnes. Inflammation. 1980;4:261–269
  91. Tagger M, Massler M. Periapical tissue reactions after pulp exposure in rat molars. Oral Surg. 1975;39:304–310
  92. Hedman WJ. An investigation into residual periapical infection after pulp canal therapy. Oral Surg. 1951;4:1173–1179
  93. Shindell E. A study of some periapical roentgenolucencies and their significance. Oral Surg. 1961;14:1057–1065
  94. Möller AJR, Fabricius L, Dahlén G, Öhman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue. Scand J Dent Res. 1981;89:475–484
  95. Fabricius L, Dahlén G, Öhman AE, Möller AJR. Predominant indigenous oral bacteria isolated from infected root canals after varied times of closure. Scand J Dent Res. 1982;90:134–144
  96. Socransky SS, Gibbons RJ. Required role of Bacteroides melaninogenicus in mixed anaerobic infections. J Infect Dis. 1965;115:247–253
  97. Sundqvist GK, Eckerborn MI, Larsson AP, Sjogren UT. Capacity of anaerobic bacteria from necrotic pulps to induce purulent infections. Infect Immun. 1979;25:685–693
  98. Pancholi V, Ayyagari AS, Agarwal KC. Experimentally induced subcutaneous infection by black-pigmented Bacteroides species in rats. J Infection. 1985;11:131–137
  99. Tronstad L, Barnett F, Flax M, Slots J. Anaerobic bacteria in periapical lesions of human teeth [Abstract]. J Dent Res. 1986;65(special issue):231
  100. Slots J, Genco RJ. Black-pigmented Bacteroides species, Capnocytophaga species and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival and tissue destruction. J Dent Res. 1984;63:412–421
  101. Handley PS, Tipler LS. An electron microscope survey of the surface structures and hydrophobicity of oral and non-oral species of the bacterial genus Bacteroides. Arch Oral Biol. 1986;31:325–335
  102. Sundqvist GK, Johansson E. Neutrophil chemotaxis induced by an-aerobic bacteria from necrotic dental pulps. Scand J Dent Res. 1980;88:113–121
  103. Moorer WR, Thoden van Velzen SK, Wesselink PR. Abscess formation induced in rabbits with bacteria-filled subcutaneous implants that simulate the infected dental root canal. Oral Surg. 1985;59:642–646
  104. van Steenbergen TJM, van Winkelhoff AJ, Mayrand D, Grenier D, de Graaf J. Bacteroides endodontalis sp. nov., an asaccharolytic black-pigmented bacteroides species from infected dental root canals. Int J Syst Bacteriol. 1984;34:118–120
  105. van Winkelhoff AJ, Martijn TJ, van Steenbergen TJM, Kippuw N, de Graaf J. Further characterization of Bacteroides endodontalis, an asaccharolytic black-pigmented bacteroides species from the oral cavity. J Clin Microbiol. 1985;22:75–79
  106. Haapasalo M, Ranta H, Shah H, Ranta K, Lounatmaa K, Kroppenstedt RM. Biochemical and structural characterization of an unusual group of Gramnegative anaerobic rods from human periapical osteitis. J Gen Microbiol. 1986;132:417–426
  107. Haapasalo M, Ranta H, Shah H, Ranta K, Lounatmaa K, Koppenstedt RM. Mitsuokella dentalis sp nov from dental root canals. Intl J Syst Bacteriol. 1986;36:566–568
  108. van Winkelhoff AJ, Carlee AW, de Graaff J. Bacteroides endodontalis and other black-pigmented bacteroides species in odontogenic abscesses. Infect Immun. 1985;49:494–497
  109. Ranta H, Lounatmaa K, Haapasalo M, Ranta K. Isolation and ultrastructure of bacteroides sp. with external layer (S-layer) in periapical osteitis. Scand J Dent Res. 1983;91:458–464
  110. Sabiston CB, Gold WA. Anaerobic bacteria in oral infections. Oral Surg. 1974;38:187–192
  111. Sabiston CB, Grigsby WR, Segerstrom N. Bacterial study of pyogenic infections of dental origin. Oral Surg. 1976;41:430–435
  112. Chow AW, Roser SM, Brady FA. Orofacial odontogenic infections. Ann Intern Med. 1978;88:392–402
  113. Kannangara DW, Thadepalli H, McQuirter JL. Bacteriology and treatment of dental infections. Oral Surg. 1980;50:103–109
  114. Aderhold L, Knothe H, Frenkel G. The bacteriology of dentogenous pyogenic infections. Oral Surg. 1981;52:583–587
  115. Labriola JD, Mascaro J, Alpert B. The microbiologic flora of orofacial abscesses. J Oral Maxillofac Surg. 1983;41:711–714
  116. von Konow L, Nord CE, Nordenram A. Anaerobic bacteria in dentoalveolar infections. Int J Oral Surg. 1981;10:313–322
  117. Brook I, Grimm S, Kielich RB. Bacteriology of acute periapical abscesses in children. J Endodon. 1981;7:378–380
  118. Oguntebi B, Slee AM, Tanzer JM, Langeland K. Predominant microflora associated with human dental periapical abscesses. J Clin Microbiol. 1982;15:964–966
  119. Williams BL, McCann GF, Shoenknecht FD. Bacteriology of dental abscesses of endodontic origin. J Clin Microbiol. 1983;18:770–774
  120. Lewis MAO, MacFarlane TW, McGowan DA. Quantitative bacteriology of acute dento-alveolar abscesses. J Med Microbiol. 1986;21:101–104
  121. Möller AJR. Microbiological examination of root canals and periapical tissues of human teeth. Odontol Tidskr. 1966;74:1–380

PII: S0099-2399(88)80200-0

doi: 10.1016/S0099-2399(88)80200-0

Journal of Endodontics
Volume 14, Issue 7 , Pages 363-371 , 1988