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Antimicrobial Activity of Maleic Acid and Combinations of Cetrimide with Chelating Agents against Enterococcus Faecalis Biofilm

Published:August 19, 2010DOI:https://doi.org/10.1016/j.joen.2010.06.009

      Abstract

      Introduction

      Chelating agents, such as EDTA or citric acid, can be incorporated into irrigants in order to provide different properties in a single compound. Maleic acid has recently been proposed as an alternative irrigant to EDTA given its better smear layer removal and biocompatibility; however, its antimicrobial activity is not known. The purpose of the present study was therefore to evaluate the in vitro capacity of maleic acid in eradicating Enterococcus faecalis biofilms and to evaluate the combinations of cetrimide with maleic acid, citric acid, and EDTA.

      Methods

      E. faecalis biofilms were grown in the MBEC high-throughput device (Innovotech; Edmonton, Alberta, Canada) for 24 hours and exposed to the irrigating solutions for 30 seconds and 1 and 2 minutes. “Eradication” was defined as 100% bacterial kill. The Student t test was used to compare the efficacy of the irrigant combinations.

      Results

      Maleic acid eradicated E. faecalis biofilms at a concentration of 0.88% after 30 seconds and at 0.11% after 2 minutes of contact time. When combined with 0.2% cetrimide, it eradicated the biofilms at all three times of exposure. The combination of 0.2% cetrimide with either 15% EDTA or 15% citric acid gave 100% bacterial kill after one minute of contact with the biofilms.

      Conclusions

      Maleic acid showed antimicrobial activity against E. faecalis biofilm both alone or in association with cetrimide from 30 seconds onward and the combination of EDTA and citric acid with cetrimide eradicated biofilm after 1 minute of contact.

      Key Words

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      References

        • Sjögren U.
        • Figdor D.
        • Persson S.
        • et al.
        Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis.
        Int Endod J. 1997; 30: 297-306
        • Waltimo T.
        • Trope M.
        • Haapasalo M.
        • et al.
        Clinical efficacy of treatment procedures in endodontic infection control and one year follow-up of periapical healing.
        J Endod. 2005; 31: 863-866
        • Sundqvist G.
        • Figdor D.
        • Persson S.
        • et al.
        Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85: 86-93
        • Sedgley C.
        • Nagel A.
        • Dahlén G.
        • et al.
        Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals.
        J Endod. 2006; 32: 173-177
        • Donlan R.M.
        • Costerton J.W.
        Biofilms: survival mechanisms of clinically relevant microorganisms.
        Clin Microbiol Rev. 2002; 15: 167-193
        • Sandoe J.A.
        • Wysome J.
        • West A.P.
        • et al.
        Measurement of ampicillin, vancomycin, linezolid and gentamicin activity against enterococcal biofilms.
        J Antimicrob Chemother. 2006; 57: 767-770
        • Zehnder M.
        Root canal irrigants.
        J Endod. 2006; 32: 389-398
        • Giardino L.
        • Ambu E.
        • Becce C.
        • et al.
        Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic.
        J Endod. 2006; 32: 1091-1093
        • Arias-Moliz M.T.
        • Ferrer-Luque C.M.
        • Espigares-García M.
        • et al.
        Enterococcus faecalis biofilms eradication by root canal irrigants.
        J Endod. 2009; 35: 711-714
        • Torabinejad M.
        • Khademi A.A.
        • Babagoli J.
        • et al.
        A new solution for the removal of the smear layer.
        J Endod. 2003; 29: 170-175
        • Dunavant T.R.
        • Regan J.D.
        • Glickman G.N.
        • et al.
        Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms.
        J Endod. 2006; 32: 527-531
        • Arias-Moliz M.T.
        • Ferrer-Luque C.M.
        • González-Rodríguez M.P.
        • et al.
        Eradication of Enterococcus faecalis biofilms by cetrimide and chlorhexidine.
        J Endod. 2010; 36: 87-90
        • Ballal N.V.
        • Kundabala M.
        • Bhat S.
        • et al.
        A comparative in vitro evaluation of cytotoxic effects of EDTA and maleic acid: root canal irrigants.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108: 633-638
        • Ballal N.V.
        • Kandian S.
        • Mala K.
        • et al.
        Comparison of the efficacy of maleic acid and ethylenediaminetetraacetic acid in smear layer removal from instrumented human root canal: a scanning electron microscopic study.
        J Endod. 2009; 35: 1573-1576
        • Gadang V.P.
        • Hettiarachchy N.S.
        • Johnson M.G.
        • et al.
        Evaluation of antibacterial activity of whey protein isolate coating incorporated with nisin, grape seed extract, malic acid, and EDTA on a Turkey frankfurter system.
        J Food Sci. 2008; 73: M389-M394
        • Ceri H.
        • Olson M.E.
        • Stremick C.
        • et al.
        The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms.
        J Clin Microbiol. 1999; 37: 1771-1776
        • Harrison J.J.
        • Turner R.J.
        • Ceri H.
        High-throughput metal susceptibility testing of microbial biofilms.
        BMC Microbiol. 2005; 3: 53
        • Haapasalo M.
        • Qian W.
        • Portenier I.
        • et al.
        Effects of dentin on the antimicrobial properties of endodontic medicaments.
        J Endod. 2007; 33: 917-925
        • Lui J.N.
        • Kuah H.G.
        • Chen N.N.
        Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer.
        J Endod. 2007; 33: 472-475
        • Crumpton B.J.
        • Goodell G.G.
        • McClanahan S.B.
        Effects on smear layer and debris removal with varying volumes of 17% REDTA after rotary instrumentation.
        J Endod. 2005; 31: 536-538
        • Calt S.
        • Serper A.
        Smear layer removal by EGTA.
        J Endod. 2000; 26: 459-461
        • Mello I.
        • Kammerer B.A.
        • Yoshimoto D.
        • et al.
        Influence of final rinse technique on ability of ethylenediaminetetraacetic acid of removing smear layer.
        J Endod. 2010; 36: 512-514
        • Prabhu S.G.
        • Rahim N.
        • Bhat K.S.
        • et al.
        Comparison of removal of endodontic smear layer using NaOCl, EDTA, and different concentrations of maleic acid—an SEM study.
        Endodontology. 2003; 15: 20-25
        • Eswaranandam S.
        • Hettiarachchy N.S.
        • Johnson M.G.
        Antimicrobial activity of citric, lactic, malic, or tartaric acids and nisin-incorporated soy protein film against Listeria monocytogenes, Escherichia coli O157: H7, and Salmonella graminara.
        J Food Sci. 2004; 69: 79-84