Abstract
Introduction
This study evaluated the in vivo response of apical and periapical tissues of dogs’ teeth with apical periodontitis
after one-session endodontic treatment with and without antimicrobial photodynamic
therapy (aPDT).
Methods
Sixty root canals with experimentally induced apical periodontitis were instrumented
and assigned to 4 groups receiving aPDT and root canal filling (RCF) or not: group
aPDT+/RCF+ (n = 20): aPDT (photosensitizer phenothiazine chloride at 10 mg/mL for
3 minutes and diode laser [λ = 660 nm, 60 mW/cm2] for 1 minute) and RCF in the same session; group aPDT+/RCF− (n = 10); group aPDT−/RCF+
(n = 20), and group aPDT−/RCF− (n = 10). Teeth were restored, and the animals were
killed after 90 days. Sections from the maxillas and mandibles were stained with hematoxylin-eosin
and Mallory trichrome and examined under light microscopy. Descriptive (ie, newly
formed apical mineralized tissue, periapical inflammatory infiltrate, apical periodontal
ligament thickness, and mineralized tissue resorption) and quantitative (ie, periapical
lesion size and number of inflammatory cells) microscopic analysis was performed.
Quantitative data were analyzed by the Kruskal-Wallis and Dunn tests (α = .05).
Results
In the aPDT-treated groups, the periapical region was moderately/severely enlarged
with no inflammatory cells, moderate neoangiogenesis and fibrogenesis, and the smallest
periapical lesions.
Conclusions
Although apical closure by mineralized tissue deposition was not achieved, the absence
of inflammatory cells, moderate neoangiogenesis, and fibrogenesis in the periapical
region in the groups treated with aPDT indicate that this can be a promising adjunct
therapy to cleaning and shaping procedures in teeth with apical periodontitis undergoing
one-session endodontic treatment.
Key Words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of EndodonticsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence.Endod Dent Traumatol. 1996; 12: 66-69
- EM evaluation of bacterial biofilm and microorganisms on the apical external root surface of human teeth.J Endod. 2002; 28: 815-818
- Histological evaluation of therapy using a calcium hydroxide dressing for teeth with incompletely formed apices and periapical lesions.J Endod. 1993; 19: 348-352
- Photodynamic therapy.J Natl Cancer Inst. 1998; 90: 889-905
- The effect of a single episode of antimicrobial photodynamic therapy in the treatment of experimental periodontitis. Microbiological profile and cytokine pattern in the dog mandible.Lasers Med Sci. 2011; 26: 359-367
- Photodynamic antimicrobial chemotherapy (PACT).J Antimicrob Chemother. 1998; 42: 13-28
- Antibacterial photodynamic therapy using water-soluble formulations of hypericin or mTHPC is effective in inactivation of Staphylococcus aureus.Photochem Photobiol Sci. 2010; 9: 365-369
- Photodynamic therapy in dentistry.J Dent Res. 2007; 86: 694-707
- Photodynamic therapy in the control of oral biofilms.Periodontol 2000. 2011; 55: 143-166
- Photodynamic effects of novel XF porphyrin derivatives on prokaryotic and eukaryotic cells.Antimicrob Agents Chemother. 2005; 49: 1542-1552
- Photodynamic inactivation of fibroblasts by a cationic porphyrin.Lasers Med Sci. 2005; 20: 62-67
- Endodontic antimicrobial photodynamic therapy: safety assessment in mammalian cell cultures.J Endod. 2009; 35: 1567-1572
- Advanced noninvasive light-activated disinfection: assessment of cytotoxicity on fibroblast versus antimicrobial activity against Enterococcus faecalis.J Endod. 2007; 33: 599-602
- Effects of growth phase and extracellular slime on photodynamic inactivation of gram-positive pathogenic bacteria.Antimicrob Agents Chemother. 2004; 48: 2173-2178
- Comparative antistreptococcal activity of photobactericidal agents.J Chemother. 2003; 15: 329-334
- Photodynamic therapy mediated by methylene blue dye in wound healing.Photomed Laser Surg. 2010; 28: 581-587
- Antimicrobial photodynamic therapy in the non-surgical treatment of aggressive periodontitis: microbiological profile.Lasers Med Sci. 2011; ([Epub ahead of print])
- Endodontic photodynamic therapy ex vivo.J Endod. 2011; 37: 217-222
- Antimicrobial effects of photodynamic therapy on patients with necrotic pulps and periapical lesion.J Endod. 2008; 34: 138-142
- Photodynamic therapy associated with conventional endodontic treatment in patients with antibiotic-resistant microflora: a preliminary report.J Endod. 2010; 36: 1463-1466
- ISO 7405: Dentistry–Preclinical evaluation of biocompatibility of medical devices used in dentistry–Test methods for dental materials.ISO, Geneva, Switzerland1997
- Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs’ teeth with apical periodontitis.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109: 779-787
- Effect of rotary or manual instrumentation, with or without a calcium hydroxide/1% chlorhexidine intracanal dressing, on the healing of experimentally induced chronic periapical lesions.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 99: 628-636
- Periapical health related to the quality of root canal treatment in a Belgian population.Int Endod J. 2000; 33: 113-120
- Periapical health and treatment quality assessment of root-filled teeth in two Canadian populations.Int Endod J. 2003; 36: 181-192
- Longitudinal study of periapical and endodontic status in a Danish population.Int Endod J. 2006; 39: 100-107
- Intracellular reactive oxygen species in monocytes generated by photosensitive chromophores activated with blue light.Dent Mater. 2008; 24: 1070-1076
- Photodynamic treatment of endodontic polymicrobial infection in vitro.J Endod. 2008; 34: 728-734
- Photodynamic therapy for root canals infected with Enterococcus faecalis.Photomed Laser Surg. 2008; 26: 209-213
- Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC.Lasers Surg Med. 2011; 43: 241-248
- Bactericidal effects of diode laser irradiation on Enterococcus faecalis using periapical lesion defect model.ISRN Dent. 2011; ([Epub ahead of print])
- Optimising single-visit disinfection with supplementary approaches: a quest for predictability.Aust Endod J. 2011; 37: 92-98
- Effect of photodynamic therapy on the healing of cutaneous third-degree-burn: histological study in rats.Lasers Med Sci. 2010; 25: 221-228
- Decreased proliferation and cellular signaling in primary dermal fibroblasts derived from diabetics versus non diabetic sibling controls.Acad Emerg Med. 2001; 8: 519
Article info
Publication history
Published online: January 27, 2012
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
