Decalcifying Effect of Different Ethylenediaminetetraacetic Acid Irrigating Solutions and Tetraclean on Root Canal Dentin



      The objective of this study was to evaluate and compare in vitro the decalcifying capability on root canal dentin of 4 different irrigating solutions.


      Twenty-five freshly extracted maxillary central incisors were selected. The canals were prepared to obtain a total of 4 samples from each root. The samples were randomly divided into 5 equal experimental groups (n = 20) according to the irrigating solutions tested (Tetraclean, Largal Ultra, 17% ethylenediaminetetraacetic acid, Tubuliclean). For each irrigating solution, the specimens were immersed for 5, 10, and 15 minutes. After exposures, the concentration of Ca2+ extracted was measured by inductively coupled plasma-atomic emission spectrometry. Data were analyzed by means of nonparametric tests, and statistical analysis was performed.


      The higher release of Ca2+ was observed in samples treated with Tetraclean; this citric acid–based agent showed significantly higher decalcifying capability. For all irrigating solutions tested, 10 minutes of application are sufficient to obtain the maximum Ca2+ release.


      The results indicated that Tetraclean could be applied to obtain more efficient removal of the smear layer and to facilitate the biomechanical instrumentation procedures.

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        • Hennequin M.
        • Pajot J.
        • Avignant D.
        Effect of different pH values of citric acid solutions on the calcium and phosphorus contents of human root dentin.
        J Endod. 1994; 20: 551-554
        • Sen B.H.
        • Wesselink P.R.
        • Turkun M.
        The smear layer: a phenomenon in root canal therapy.
        Int Endod J. 1995; 2: 141-148
        • Doğan H.
        • Calt S.
        Effect of chelating agents and sodium hypochlorite on mineral content of root dentin.
        J Endod. 2001; 27: 578-580
        • Sim T.P.
        • Knowles J.C.
        • Shelton J.
        • Gulabilava K.
        Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain.
        Int Endod J. 2001; 34: 120-132
        • McComb D.
        • Smith D.C.
        A preliminary scanning electron microscopic study of root canal after endodontic procedures.
        J Endod. 1975; 1: 238-242
        • Scelza M.F.Z.
        • Teixeira A.M.
        • Scelza P.
        Decalcifyng effect of EDTA-T, 10% citric acid, and 17% EDTA on root canal dentin.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95: 234-236
        • O'Connell M.S.
        • Morgan L.A.
        • Beeler W.J.
        • Baumgartner J.A.
        A comparative study of smear layer removal using different salts of EDTA.
        J Endod. 2000; 26: 739-743
        • Scelza M.F.Z.
        • Daniel R.L.D.P.
        • Santos E.M.
        • Jaeger M.M.M.
        Cytotoxic effects of 10% citric acid and EDTA-T used as root canal irrigants: an in vitro analysis.
        J Endod. 2001; 27: 741-743
        • Hülsmann M.
        • Heckendorff M.
        • Schäfers F.
        Comparative in-vitro evaluation of three chelator pastes.
        Int Endod J. 2002; 35: 668-679
        • Segura-Egea J.J.
        • Jimenez-Rubio A.
        • Rios-Santos J.V.
        • Velasco-Ortega E.
        • Calvo-Gutierrez J.R.
        In vitro inhibitory effect of EGTA on macrophage adhesion: endodontic implications.
        J Endod. 2003; 29: 211-213
        • Ferrer-Luque C.M.F.
        • González-López S.G.
        • Rodrígues de Mondelo J.M.N.
        Utilización del ácido cítrico en la preparación biomecánica del conducto radicular.
        Endodoncia. 1994; 12: 63-70
        • Garberoglio R.
        • Becce C.
        Smear layer removal by root canal irrigants: a comparative scanning electron microscopic study.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1994; 78: 359-367
        • Di Lenarda R.
        • Cadenaro M.
        • Sbaizero O.
        Effectiveness of 1 mol L−1 citric acid and 15% EDTA irrigation on smear layer removal.
        Int Endod J. 2000; 33: 46-52
        • Haznedaroglu F.
        Efficacy of various concentrations of citric acid at different pH values for smear layer removal.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96: 340-344
        • Machado-Silveiro L.F.
        • González-López S.
        • González-Rodríguez M.P.
        Decalcification of root canal dentine by citric acid, EDTA and sodium citrate.
        Int Endod J. 2004; 37: 365-369
        • Calt S.
        • Serper A.
        Smear layer removal by EGTA.
        J Endod. 2000; 26: 459-461
        • Perez-Heredia M.
        • Ferrer-Luque C.M.
        • Gonzalez-Rodriguez M.P.
        The effectiveness of different acid irrigating solutions in root canal cleaning after hand and rotary instrumentation.
        J Endod. 2006; 32: 993-997
        • Yamaguchi M.
        • Yoshida K.
        • Suzuki R.
        • Nakamura H.
        Root canal irrigation with citric acid solution.
        J Endod. 1996; 22: 27-29
        • Calt S.
        • Serper A.
        Time-dent effects of EDTA on dentin structures.
        J Endod. 2002; 28: 17-19
        • Serper A.
        • Calt S.
        The demineralizing effects of EDTA at different concentrations and pH.
        J Endod. 2002; 28: 501-502
        • Beltz R.E.
        • Torabinejad M.
        • Pouresmail M.
        Quantitative analysis of the solubilizing action of MTAD, sodium hypochlorite, and EDTA on bovine pulp and dentin.
        J Endod. 2003; 29: 334-337
        • Cruz-Filho A.M.
        • Sousa-Neto M.D.
        • Savioli R.N.
        • Silva R.G.
        • Vansan L.P.
        • Pecora J.D.
        Effect of chelating solutions on the microhardness of root canal lumen dentin.
        J Endod. 2011; 37: 358-362
        • Torabinejad M.
        • Khademi A.A.
        • Babagoli J.
        • et al.
        A new solution to remove the smear layer.
        J Endod. 2003; 29: 170-175
        • Giardino L.
        • Ambu E.
        • Becce C.
        • Rimondini L.
        • Morra M.
        Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic.
        J Endod. 2006; 32: 1091-1093
        • Ingle J.I.
        Ingle's endodontics.
        BC Decker Inc Publisher, Hamilton, Ontario, Canada2008
        • Spanò J.C.
        • Silva R.
        • Guedes D.
        • Sousa-Neto M.
        • Estrela C.
        • Pécora J.
        Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators.
        J Endod. 2009; 35: 727-730
        • Snedecor G.W.
        • Cochran W.G.
        Statistical methods.
        8th ed. Iowa State University Press, Ames, IA1989
        • Poggio C.
        • Dagna A.
        • Chiesa M.
        • et al.
        SEM evaluation of the root canal walls after treatment with Tetraclean.
        Int J Artif Organs. 2010; 33: 660-666
        • Giardino L.
        • Ambu E.
        • Savoldi E.
        • Rimondini L.
        • Cassinelli C.
        • Debbia E.A.
        Comparative evaluation of antimicrobial efficacy of sodium hypochlorite, MTAD, and Tetraclean against Enterococcus faecalis biofilm.
        J Endod. 2007; 33: 852-855
        • Neglia R.
        • Ardizzoni A.
        • Giardino L.
        • et al.
        Comparative in vitro and ex vivo studies on the bactericidal activity of Tetraclean, a new generation endodontic irrigant, and sodium hypochlorite.
        New Microbiol. 2008; 31: 57-65
        • Torabinejad M.
        • Cho Y.
        • Khademi A.A.
        • Bekland L.K.
        • Shabahang S.
        The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer.
        J Endod. 2003; 29: 233-239
        • Wu L.
        • Mu Y.
        • Deng X.
        • Zhang S.
        • Zhou D.
        Comparison of the effect of four decalcifying agents combined with 60 degrees C 3% sodium hypochlorite on smear layer removal.
        J Endod. 2012; 38: 381-384
        • Caron G.
        • Nham K.
        • Bronnec F.
        • Machtou P.
        Effectiveness of different final irrigant activation protocols on smear layer removal in curved canals.
        J Endod. 2010; 36: 1361-1366
        • Sterrett J.D.
        • Delaney B.
        • Rizkalla A.
        • Hawkins C.H.
        Optimal citric acid concentration for dentinal demineralization.
        Quintessence Int. 1991; 22: 371-375