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Revascularization with and without Platelet-rich Plasma in Nonvital, Immature, Anterior Teeth: A Pilot Clinical Study

  • Ganesh Jadhav
    Affiliations
    Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India
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  • Naseem Shah
    Correspondence
    Address requests for reprints to Dr Naseem Shah, Department of Conservative Dentistry and Endodontics and Chief, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi 110 029, India.
    Affiliations
    Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India
    Search for articles by this author
  • Ajay Logani
    Affiliations
    Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India
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Published:October 22, 2012DOI:https://doi.org/10.1016/j.joen.2012.09.010

      Abstract

      Introduction

      This study was conducted to evaluate and compare maturogenesis induced by revascularization with and without platelet-rich plasma (PRP).

      Methods

      Twenty patients with nonvital, immature anterior teeth were randomly categorized into 2 groups. Subsequent to chemomechanical preparation, revascularization with and without PRP carried on a collagen sponge was induced in groups 1 and 2, respectively. The cases were followed up clinically and radiographically at 6- and 12-month intervals.

      Results

      Clinically, all cases were asymptomatic with complete resolution of signs and symptoms. Radiographically, there was a marked difference in periapical healing, apical closure, and dentinal wall thickening in group 2 in comparison with group 1. However, root lengthening was comparable for both of the procedures.

      Conclusions

      Revascularization is a conservative and an effective method for inducing maturogenesis in nonvital, immature teeth. Supplementations with PRP can potentially improve the desired biological outcome of this regenerative technique.

      Key Words

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      References

        • Murray P.E.
        • Garcia-Godoy Franklin
        • Hargreaves K.
        Regenerative endodontics-a review of current status and a call for action.
        J Endod. 2007; 33: 337-390
        • Shah N.
        • Logani A.
        • Bhaskar U.
        • Aggarwal V.
        Efficacy of revascularization to induce apexification or apexogenesis in infected, nonvital, immature teeth: a pilot clinical study.
        J Endod. 2008; 34: 919-925
        • Hargreaves K.
        • Geisler T.
        • Henry M.
        Regeneration potential of the permanent tooth: what does the future hold?.
        J Endod. 2008; 34: 51-56
        • Thibodeau B.
        • Teixeira F.
        • Yamauchi M.
        • et al.
        Pulp revascularization of immature dog teeth with apical periodontitis.
        J Endod. 2007; 33: 680-689
        • Ding R.Y.
        • Cheung G.S.P.
        • Chen J.
        Pulp revascularization of immature teeth with apical periodontitis: a clinical study.
        J Endod. 2009; 35: 745-749
        • Marx R.E.
        • Carlson E.R.
        • Eichstaedt R.M.
        • et al.
        Platelet-rich plasma: growth factor enhancement for bone grafts.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85: 638-646
        • Sato T.
        • Hoshino E.
        • Uematsu H.
        • et al.
        In vitro antimicrobial susceptibility to combinations of drugs on bacteria from carious and endodontic lesions of human deciduous teeth.
        Oral Microbiol Immunol. 1993; 8: 172-176
        • Hoshino E.
        • Kurihara-Ando N.
        • Sato I.
        • et al.
        In vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline.
        Int Endod J. 1996; 29: 125-130
        • Cvek M.
        • Cleaton-Jones P.
        • Austin J.
        • et al.
        Effect of topical application of doxycycline on pulp revascularization and periodontal healing in reimplanted monkey incisors.
        Endod Dent Traumatol. 1990; 6: 170-176
        • Heithersay G.S.
        Stimulation of root formation in incompletely developed pulpless teeth.
        Oral Surg Oral Med Oral Pathol. 1970; 29: 620-630
        • Nevins A.
        • Wrobel W.
        • Valachovic R.
        • et al.
        Hard tissue induction into pulpless open-apex teeth using collagen-calcium phosphate gel.
        J Endod. 1977; 3: 431-433
        • Lieberman J.
        Apical closure of nonvital permanent incisor teeth where no treatment was performed: a case report.
        J Endod. 1983; 9: 257-260
        • Gronthos S.
        • Mankani M.
        • Brahim J.
        • et al.
        Postnatal human dental pulp stem cells (DPSC) in vitro and in vivo.
        Proc Natl Acad Sci U S A. 2000; 97: 13625-13630
        • Krebsbach P.
        Bone formation in vivo: comparison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts.
        Transplantation. 1997; 63: 1059-1069
        • Petrino J.
        • Boda K.
        • Shambarger S.
        • et al.
        Challenges in regenerative endodontics: a case series.
        J Endod. 2010; 36: 536-541
        • Nosrat A.
        • Seifi A.
        • Asgary S.
        Regenerative endodontic treatment (revascularization) for necrotic immature permanent molars: a review and report of two cases with a new biomaterial.
        J Endod. 2011; 37: 562-567
        • Nevins A.
        • Finkelstein F.
        • Laporta R.
        • Borden B.G.
        Induction of hard tissue into pulpless open-apex teeth using collagen-calcium phosphate gel.
        J Endod. 1978; 4: 76-81
        • Torneck C.D.
        Reaction of rat connective tissue to polyethylene tube implants.
        II. Oral Surg Oral Med Oral Pathol. 1967; 24: 674-683
        • Brass L.
        Understanding and evaluating platelet function.
        Hematology Am Soc Hematol Educ Program. 2010; 2010: 387-396
        • Tewari S.
        • Goyal B.
        • Duhan J.
        • et al.
        Comparative evaluation of platelet-rich plasma and guided tissue regeneration membrane in the healing of apicomarginal defects: a clinical study.
        J Endod. 2011; 37: 773-780