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Efficacy of Instrumentation Techniques and Irrigation Regimens in Reducing the Bacterial Population within Root Canals

  • José F. Siqueira Jr
    Correspondence
    Address requests for reprints to Dr. José F. Siqueira, Jr., R. Herotides de Oliveira 61/601, Icaraí, Niterói, RJ, Brazil 24230-230.
    Affiliations
    Drs. Siqueira and Uzeda and Mr. Magalhães are members of the Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil. Dr. Siqueira is chairman and professor of Endodontics, and Dr. Rôças is professor of Endodontics, Department of Dentistry, Estácio de Sá University, RJ, Brazil. Dr. Santos is practicing in Niterói, RJ, Brazil. Dr. Lima is professor of Preventive Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
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  • Isabela N. Rôças
    Affiliations
    Drs. Siqueira and Uzeda and Mr. Magalhães are members of the Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil. Dr. Siqueira is chairman and professor of Endodontics, and Dr. Rôças is professor of Endodontics, Department of Dentistry, Estácio de Sá University, RJ, Brazil. Dr. Santos is practicing in Niterói, RJ, Brazil. Dr. Lima is professor of Preventive Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
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  • Sandra R.L.D. Santos
    Affiliations
    Drs. Siqueira and Uzeda and Mr. Magalhães are members of the Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil. Dr. Siqueira is chairman and professor of Endodontics, and Dr. Rôças is professor of Endodontics, Department of Dentistry, Estácio de Sá University, RJ, Brazil. Dr. Santos is practicing in Niterói, RJ, Brazil. Dr. Lima is professor of Preventive Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
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  • Kenio C. Lima
    Affiliations
    Drs. Siqueira and Uzeda and Mr. Magalhães are members of the Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil. Dr. Siqueira is chairman and professor of Endodontics, and Dr. Rôças is professor of Endodontics, Department of Dentistry, Estácio de Sá University, RJ, Brazil. Dr. Santos is practicing in Niterói, RJ, Brazil. Dr. Lima is professor of Preventive Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
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  • Fernando A.C. Magalhães
    Affiliations
    Drs. Siqueira and Uzeda and Mr. Magalhães are members of the Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil. Dr. Siqueira is chairman and professor of Endodontics, and Dr. Rôças is professor of Endodontics, Department of Dentistry, Estácio de Sá University, RJ, Brazil. Dr. Santos is practicing in Niterói, RJ, Brazil. Dr. Lima is professor of Preventive Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
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  • Milton de Uzeda
    Affiliations
    Drs. Siqueira and Uzeda and Mr. Magalhães are members of the Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil. Dr. Siqueira is chairman and professor of Endodontics, and Dr. Rôças is professor of Endodontics, Department of Dentistry, Estácio de Sá University, RJ, Brazil. Dr. Santos is practicing in Niterói, RJ, Brazil. Dr. Lima is professor of Preventive Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
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      The purpose of this study was to compare the in vitro intracanal bacterial reduction produced by using two instrumentation techniques and different irrigation methods. Root canals inoculated with Enterococcus faecalis were prepared by using the following techniques and irrigants: alternated rotary motions (ARM) technique, hand nickel-titanium files and 2.5% sodium hypochlorite (NaOCl) as irrigant; ARM technique and combined irrigation with 2.5% NaOCl and citric acid; ARM technique and combined irrigation with 2.5% NaOCl and 2% chlorhexidine gluconate; and Greater Taper rotary files, using 2.5% NaOCl as irrigant. Controls were instrumented by using the ARM technique and irrigated with sterile saline. Canals were sampled before and after preparation. After serial dilution, samples were plated onto Mitis-Salivarius agar, and the colony forming units that were grown were counted. All test techniques and solutions significantly reduced the number of bacterial cells within the root canal (p < 0.05). There was no significant difference between the experimental groups (p > 0.05). Nonetheless, all of them were significantly more effective than the control group (p < 0.05). These findings support the importance of using antimicrobial irrigants during the chemomechanical preparation, regardless of the solutions or instrumentation techniques used.
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