Abstract
Mineral trioxide aggregate (MTA) and Portland cement are being used in dentistry as
root end-filling materials. However, biocompatibility data concerning genotoxicity
and cytotoxicity are needed for complete risk assessment of these compounds. In the
present study, genotoxic and cytotoxic effects of MTA and Portland cements were evaluated
in vitro using the alkaline single cell gel (comet) assay and trypan blue exclusion
test, respectively, on mouse lymphoma cells. The results demonstrated that the single
cell gel (comet) assay failed to detect DNA damage after a treatment of cells by MTA
and Portland cements for concentrations up to 1000 μg/ml. Similarly, results showed
that none of the compounds tested were cytotoxic. Taken together, these results seem
to indicate that MTA and Portland cements are not genotoxins and do not induce cellular
death.
Key Words
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© 2005 The American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
