Basic research—technology| Volume 33, ISSUE 12, P1429-1432, December 2007

Download started.


Sealing Ability of White and Gray Mineral Trioxide Aggregate Mixed with Distilled Water and 0.12% Chlorhexidine Gluconate When Used as Root-end Filling Materials

Published:September 18, 2007DOI:


      This in vitro study used dye penetration to compare the sealing ability of white and gray mineral trioxide aggregate mixed with distilled water and 0.12% chlorhexidine gluconate when used as root-end filling materials. Ninety-six single-rooted human teeth were cleaned, shaped, and obturated with gutta-percha and AH26 root canal sealer. The apical 3 mm of each root was resected, and 3-mm deep root-end cavity preparations were made. The teeth were randomly divided into 4 experimental groups, each containing 20 teeth, and 2 negative and positive control groups, each containing 8 teeth. Root-end cavities in the experimental groups were filled with the experimental materials. After decoronation of the teeth and application of nail polish, the teeth were exposed to India ink for 72 hours and longitudinally sectioned, and the extent of dye penetration was measured with a stereomicroscope. Statistical analysis showed that there were no significant differences among the 4 experimental groups.

      Key Words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Journal of Endodontics
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Ingle J.I.
        • Simon J.H.
        • Machtou P.
        • Bogaerts P.
        Outcome of endodontic treatment and re-treatment.
        in: Ingle J.I. Backland L.K. Endodontics. 5th ed. Lea & Febiger, Philadelphia2002: 751-752
        • Friedman S.
        Retrograde approaches in endodontic therapy.
        Endod Dent Traumatol. 1991; 7: 97-107
        • Johnson B.R.
        • Witherspoon D.E.
        Periradicular surgery.
        in: Cohen S. Hargreaves K.M. Pathways of the pulp. 9th ed. C V Mosby, St Louis2006: 756
        • Lee S.J.
        • Monsef M.
        • Torabinejad M.
        Sealing ability of mineral trioxide aggregate for repair of lateral root perforations.
        J Endod. 1993; 19: 541-544
        • Torabinejad M.
        • Watson T.F.
        • Pitt Ford T.R.
        Sealing ability of a mineral trioxide aggregate when used as a root end filling material.
        J Endod. 1993; 19: 591-595
        • Torabinejad M.
        • Higa R.K.
        • McKendry D.J.
        • Pitt Ford T.R.
        Dye leakage of four root end filling materials: effects of blood contamination.
        J Endod. 1994; 20: 159-163
        • Torabinejad M.
        • Hong C.U.
        • Pitt Ford T.R.
        • Kaiyawasam S.P.
        Tissue reaction to implanted Super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report.
        J Endod. 1995; 21: 569-571
        • Torabinejad M.
        • Rastegar A.F.
        • Kettering J.D.
        • Pitt Ford T.R.
        Bacterial leakage of mineral trioxide aggregate as a root- end filling material.
        J Endod. 1995; 21: 109-112
        • Torabinejad M.
        • Smith P.W.
        • Kettering J.D.
        • Pitt Ford T.R.
        Comparative investigation of marginal adaptation of mineral trioxide aggregate and other commonly used root-end filling materials.
        J Endod. 1995; 21: 295-299
        • Wu M.K.
        • Kontakiods E.G.
        • Wesselink P.R.
        Long-term seal provided by some root-end filling materials.
        J Endod. 1998; 24: 557-560
        • Adamo H.L.
        • Buruiana R.
        • Schertzer L.
        • Boylan R.J.
        A comparison of MTA, Super-EBA, composite and amalgam as root-end filling materials using a bacterial microleakage model.
        Int Endod J. 1999; 32: 197-203
        • Daoudi M.F.
        • Saunders W.P.
        In vitro evaluation of furcal perforation repair using mineral trioxide aggregate or resin-modified glass ionomer cement with and without the use of the operating microscope.
        J Endod. 2002; 28: 512-515
        • Holland R.
        • Souza V.
        • Nery M.J.
        • et al.
        Reaction of rat connective tissue to implanted dentine tubes filled with a white mineral trioxide aggregate.
        Braz Dent J. 2002; 13: 23-26
        • Perez A.L.
        • Spears R.
        • Gutmann J.L.
        • Opperman L.A.
        Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with pro root MTA and white MTA.
        Int Endod J. 2003; 36: 564-570
        • Mah T.
        • Basrani B.
        • Santos J.M.
        • et al.
        Periapical inflammation affecting coronally-inoculated dog teeth with root fillings augmented by white MTA orifice plugs.
        J Endod. 2003; 29: 442-446
        • Shahi S.
        • Rahimi S.
        • Lotfi M.
        • Yavari H.R.
        • Gaderian A.R.
        A comparative study of the biocompatibility of three root-end filling materials in rat connective tissue.
        J Endod. 2006; 32: 776-780
        • Stowe T.J.
        • Sedgley C.M.
        • Stowe B.
        • Fenno J.C.
        The effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored ProRoot mineral trioxide aggregate.
        J Endod. 2004; 30: 429-431
        • Yang P.
        • Peng B.
        • Fan B.
        • Fan M.
        • Bian Z.
        The effects of sodium hypochlorite (5.25%), chlorhexidine (2%), and Glyde File Prep on the bond strength of MTA-dentin.
        J Endod. 2006; 32: 58-60
        • Taylor M.J.
        • Lynch E.
        J Dentist. 1992; 20: 3-10
        • Oztan M.D.
        • Ozgey E.
        • Zaimoglu L.
        • Erk N.
        Effect of particle sizes in India ink on its use in evaluation of apical seal.
        J Oral Sci. 2001; 43: 245-248
        • Yashikawa M.
        • Nogochi K.
        • Toda T.
        Effect of particle sizes in India ink on its use in evaluation of apical seal.
        J Osaka Dent Univ. 1997; 31: 67-70
        • Higa R.K.
        • Torabinejad M.
        • McKendry D.J.
        • McMillan P.J.
        The effect of storage time on the degree of dye leakage of root-end filling materials.
        Int Endod J. 1994; 27: 252-256
        • Torabinejad M.
        • Pitt Ford T.R.
        • McKendry D.J.
        • Abedi H.R.
        • Miller D.A.
        • Kariyawasam S.P.
        Histologic assessment of mineral trioxide aggregate as a root-end filling in monkeys.
        J Endod. 1997; 23: 225-228
        • Estrela C.
        • Bammann L.L.
        • Estrela C.R.
        • Silva R.S.
        • Recora J.D.
        Antimicrobial and chemical study of MTA, Portland cement, calcium hydroxide paste, Sealapex and Dycal.
        Braz Dent J. 2000; 11: 3-9
        • Matt G.D.
        • Thorpe J.R.
        • Strother J.M.
        • McClanahan S.B.
        Comparative study of white and gray mineral trioxide aggregate (MTA) simulating a one-or two step apical barrier technique.
        J Endod. 2004; 30: 876-879
        • Kogan P.
        • He J.
        • Glickman G.N.
        • Watanabe I.
        The effects of various additives on setting properties of MTA.
        J Endod. 2006; 32: 569-572
        • Sumer M.
        • Muglali M.
        • Bodrumlu E.
        • Guvenc T.
        Reactions of connective tissue to amalgam, intermediate restorative material, mineral trioxide aggregate and mineral trioxide aggregate mixed with chlorhexidine.
        J Endod. 2006; 32: 1094-1096