Abstract
The purpose of this study was to investigate the effects of mineral trioxide aggregate
(MTA) on survival, mineralization, and expression of mineralization-related genes
of cementoblasts. Immortalized cementoblasts (OCCM) were maintained with Dulbecco
modified Eagle medium containing 10% fetal bovine serum. Methyl-thiazol-diphenyl-tetrazolium
experiments were performed at 24 and 72 hours to evaluate bioactive components released
by MTA (0.002-20 mg/mL) on the cell survival of OCCM. Von Kossa staining was used
to evaluate biomineralization of OCCM cells. Images of cementoblasts were taken on
day 3 by using inverted microscopy. Gene transcripts for bone sialoprotein (BSP),
OCN, collagen type I (COL I), and osteopontin (OPN) were evaluated on days 3 and 5
by using semiquantitative reverse transcriptase polymerase chain reaction. The 20
mg/mL concentration of MTA was toxic for OCCM cells, whereas other concentrations
of MTA tested exhibited similar cell numbers when compared with control group, and
the 0.02 mg/mL concentration of MTA increased OCCM cell survival at 72 hours. Although
an apparent decrease in mineralization was observed in the highest 3 concentrations
of MTA used, 0.02 and 0.002 mg/mL concentrations of MTA induced greater biomineralization
of OCCM cells than seen in the control. Moreover, increased BSP and COL I mRNA expression
was observed at 0.02 and 0.002 mg/mL concentrations of MTA. MTA did not have a negative
effect on the viability and morphology of cementoblasts and induced biomineralization
of cementoblasts at the concentrations of 0.02 and 0.002 mg/mL. Based on these results
MTA can be considered as a favorable material regarding cell-material interaction.
Key Words
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© 2009 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
