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Review Article| Volume 36, ISSUE 2, P190-202, February 2010

Mineral Trioxide Aggregate: A Comprehensive Literature Review—Part II: Leakage and Biocompatibility Investigations

  • Mahmoud Torabinejad
    Correspondence
    Address requests for reprints to Mahmoud Torabinejad, DMD, MSD, PhD, Professor and Director, Endodontic Residency Program, Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, CA 92350.
    Affiliations
    Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, California
    Search for articles by this author
  • Masoud Parirokh
    Affiliations
    Department of Endodontics, School of Dentistry, Oral and Dental Diseases Research Center, Neuroscience Research Center, Iranian Center for Endodontic Research, Kerman University of Medical Sciences, Kerman, Iran
    Search for articles by this author

      Abstract

      Introduction

      Mineral trioxide aggregate (MTA) was developed because existing materials did not have the ideal characteristics for orthograde or retrograde root-end fillings. MTA has been recommended primarily as a root-end filling material, but it has also been used in pulp capping, pulpotomy, apical barrier formation in teeth with open apexes, repair of root perforations, and root canal filling. Part I of this literature review presented a comprehensive list of articles regarding the chemical and physical properties as well as the antibacterial activity of MTA. The purpose of part II of this review is to present a comprehensive list of articles regarding the sealing ability and biocompatibility of this material.

      Methods

      A review of the literature was performed by using electronic and hand-searching methods for the sealing ability and biocompatibility of MTA from November 1993–September 2009.

      Results

      Numerous studies have investigated the sealing ability and biocompatibility of MTA.

      Conclusions

      On the basis of available evidence it appears that MTA seals well and is a biocompatible material.

      Key Words

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      References

        • Ng Y.L.
        • Mann V.
        • Rahbaran S.
        • Lewsey J.
        • Gulabivala K.
        Outcome of primary root canal treatment: systematic review of the literature—part 2: influence of clinical factors.
        Int Endod J. 2008; 41: 6-31
        • Torabinejad M.
        • Pitt Ford T.R.
        Root end filling materials. a review.
        Endod Dent Traumatol. 1996; 12: 161-178
        • Parirokh M.
        • Torabinejad M.
        • Mineral trioxide aggregate (MTA)
        a comprehensive literature review—part I: chemical, physical & antibacterial properties.
        J Endodo. 2010; 36: 16-27
        • Torabinejad M.
        • Watson T.F.
        • Pitt Ford T.R.
        Sealing ability of a mineral trioxide aggregate when used as a root end filling material.
        J Endod. 1993; 19: 591-595
        • Torabinejad M.
        • Higa R.K.
        • McKendry D.J.
        • Pitt Ford T.R.
        Dye leakage of four root end filling materials: effects of blood contamination.
        J Endod. 1994; 20: 159-163
        • Aqrabawi J.
        Sealing ability of amalgam, super EBA cement, and MTA when used as retrograde filling materials.
        Br Dent J. 2000; 188: 266-268
        • Martell B.
        • Chandler N.P.
        Electrical and dye leakage comparison of three root-end restorative materials.
        Quintessence Int. 2002; 33: 30-34
        • Andelin W.E.
        • Browning D.F.
        • Hsu G.H.
        • Roland D.D.
        • Torabinejad M.
        Microleakage of resected MTA.
        J Endod. 2002; 28: 573-574
        • Davis J.L.
        • Jeansonne B.G.
        • Davenport W.D.
        • Gardiner D.
        The effect of irrigation with doxycycline or citric acid on leakage and osseous wound healing.
        J Endod. 2003; 29: 31-35
        • Pereira C.L.
        • Cenci M.S.
        • Demarco F.F.
        Sealing ability of MTA, Super EBA, Vitremer and amalgam as root-end filling materials.
        Braz Oral Res. 2004; 18: 317-321
        • Asgary S.
        • Eghbal M.J.
        • Parirokh M.
        Sealing ability of a novel endodontic cement as a root-end filling material.
        J Biomed Mater Res. 2008; 87A: 706-709
        • Tanomaru Filho M.
        • Figueiredo F.A.
        • Tanomaru J.M.
        Effect of different dye solutions on the evaluation of the sealing ability of mineral trioxide aggregate.
        Braz Oral Res. 2005; 19: 119-122
        • Kubo C.H.
        • Gomes A.P.
        • Mancini M.N.
        In vitro evaluation of apical sealing in root apex treated with demineralization agents and retrofiled with mineral trioxide aggregate through marginal dye leakage.
        Braz Dent J. 2005; 16: 187-191
        • Vogt B.F.
        • Xavier C.B.
        • Demarco F.F.
        • Padilha M.S.
        Dentin penetrability evaluation of three different dyes in root-end cavities filled with mineral trioxide aggregate (MTA).
        Braz Oral Res. 2006; 20: 132-136
        • Pichardo M.R.
        • George S.W.
        • Bergeron B.E.
        • Jeansonne B.G.
        • Rutledge R.
        Apical leakage of root-end placed SuperEBA, MTA, and Geristore restorations in human teeth previously stored in 10% formalin.
        J Endod. 2006; 32: 956-959
        • Tobón-Arroyave S.I.
        • Restrepo-Pérez M.M.
        • Arismendi-Echavarría J.A.
        • Velásquez-Restrepo Z.
        • Marín-Botero M.L.
        • García-Dorado E.C.
        Ex vivo microscopic assessment of factors affecting the quality of apical seal created by root-end fillings.
        Int Endod J. 2007; 40: 590-602
        • Gondim Jr., E.
        • Kim S.
        • de Souza-Filho F.J.
        An investigation of microleakage from root-end fillings in ultrasonic retrograde cavities with or without finishing: a quantitative analysis.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 99: 755-760
        • Abedi H.R.
        • Van Mierlo B.L.
        • Wilder-Smith P.
        • Torabinejad M.
        Effects of ultrasonic root-end cavity preparation on the root apex.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995; 80: 207-213
        • Roy C.O.
        • Jeansonne B.G.
        • Gerrets T.F.
        Effect of an acid environment on leakage of root-end filling materials.
        J Endod. 2001; 27: 7-8
        • Wu M.K.
        • Kontakiotis E.G.
        • Wesselink P.R.
        Decoloration of 1% methylene blue solution in contact with dental filling materials.
        J Dent. 1998; 26: 585-589
        • Camilleri J.
        • Pitt Ford T.R.
        Evaluation of the effect of tracer pH on the sealing ability of glass ionomer cement and mineral trioxide aggregate.
        J Mater Sci Mater Med. 2008; 19: 2941-2948
        • Bates C.F.
        • Carnes D.L.
        • del Rio C.E.
        Longitudinal sealing ability of mineral trioxide aggregate as a root-end filling material.
        J Endod. 1996; 22: 575-578
        • Yatsushiro J.D.
        • Baumgartner J.C.
        • Tinkle J.S.
        Longitudinal study of the microleakage of two root-end filling materials using a fluid conductive system.
        J Endod. 1998; 24: 716-719
        • Wu M.K.
        • Kontakiotis E.G.
        • Wesselink P.R.
        Long-term seal provided by some root-end filling materials.
        J Endod. 1998; 24: 557-560
        • Fogel H.M.
        • Peikoff M.D.
        Microleakage of root-end filling materials.
        J Endod. 2001; 27 (Erratum in: J Endod 2001;27:634): 456-458
        • Karlovic Z.
        • Pezelj-Ribaric S.
        • Miletic I.
        • Jukic S.
        • Grgurevic J.
        • Anic I.
        Erbium:YAG laser versus ultrasonic in preparation of root-end cavities.
        J Endod. 2005; 31: 821-823
        • Lamb E.L.
        • Loushine R.J.
        • Weller R.N.
        • Kimbrough W.F.
        • Pashley D.H.
        Effect of root resection on the apical sealing ability of mineral trioxide aggregate.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95: 732-735
        • De Bruyne M.A.
        • De Bruyne R.J.
        • Rosiers L.
        • De Moor R.J.
        Longitudinal study on microleakage of three root-end filling materials by the fluid transport method and by capillary flow porometry.
        Int Endod J. 2005; 38: 129-136
        • De Bruyne M.A.
        • De Bruyne R.J.
        • De Moor R.J.
        Capillary flow porometry to assess the seal provided by root-end filling materials in a standardized and reproducible way.
        J Endod. 2006; 32: 206-209
        • Nakamichi I.
        • Iwaku M.
        • Fusayama T.
        Bovine teeth as possible substitutes in the adhesion test.
        J Dent Res. 1983; 62: 1076-1081
        • Valois C.R.
        • Costa Jr., E.D.
        Influence of the thickness of mineral trioxide aggregate on sealing ability of root-end fillings in vitro.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97: 108-111
        • Saghiri M.A.
        • Lotfi M.
        • Saghiri A.M.
        • Vosoughhosseini S.
        • Fatemi A.
        • Shiezadeh V.
        • Ranjkesh B.
        Effect of pH on sealing ability of white mineral trioxide aggregate as a root-end filling material.
        J Endod. 2008; 34: 1226-1229
        • Torabinejad M.
        • Rastegar A.F.
        • Kettering J.D.
        • Pitt Ford T.R.
        Bacterial leakage of mineral trioxide aggregate as a root-end filling material.
        J Endod. 1995; 21: 109-112
        • Fischer E.J.
        • Arens D.E.
        • Miller C.H.
        Bacterial leakage of mineral trioxide aggregate as compared with zinc-free amalgam, intermediate restorative material, and Super-EBA as a root-end filling material.
        J Endod. 1998; 24: 176-179
        • Adamo H.L.
        • Buruiana R.
        • Schertzer L.
        • Boylan R.J.
        A comparison of MTA, Super-EBA, composite and amalgam as root-end filling materials using a bacterial microleakage model.
        Int Endod J. 1999; 32: 197-203
        • Scheerer S.Q.
        • Steiman H.R.
        • Cohen J.
        A comparative evaluation of three root-end filling materials: an in vitro leakage study using Prevotella nigrescens.
        J Endod. 2001; 27: 40-42
        • Mangin C.
        • Yesilsoy C.
        • Nissan R.
        • Stevens R.
        The comparative sealing ability of hydroxyapatite cement, mineral trioxide aggregate, and super ethoxybenzoic acid as root-end filling materials.
        J Endod. 2003; 29: 261-264
        • Maltezos C.
        • Glickman G.N.
        • Ezzo P.
        • He J.
        Comparison of the sealing of Resilon, Pro Root MTA, and Super-EBA as root-end filling materials: a bacterial leakage study.
        J Endod. 2006; 32: 324-327
        • Montellano A.M.
        • Schwartz S.A.
        • Beeson T.J.
        Contamination of tooth-colored mineral trioxide aggregate used as a root-end filling material: a bacterial leakage study.
        J Endod. 2006; 32: 452-455
        • Ferk Luketić S.
        • Malcić A.
        • Jukić S.
        • Anić I.
        • Segović S.
        • Kalenić S.
        Coronal microleakage of two root-end filling materials using a polymicrobial marker.
        J Endod. 2008; 34: 201-203
        • Tang H.M.
        • Torabinejad M.
        • Kettering J.D.
        Leakage evaluation of root end filling materials using endotoxin.
        J Endod. 2002; 28: 5-7
        • Torabinejad M.
        • Ung B.
        • Kettering J.D.
        In vitro bacterial penetration of coronally unsealed endodontically treated teeth.
        J Endod. 1990; 16: 566-569
        • Parirokh M.
        • Askarifard S.
        • Mansouri S.
        • Haghdoost A.A.
        • Raoof M.
        • Torabinejad M.
        Effect of phosphate buffer saline on coronal leakage of mineral trioxide aggregate.
        J Oral Sci. 2009; 51: 187-191
        • Fernández-Yáñez Sánchez A.
        • Leco-Berrocal M.I.
        • Martínez-González J.M.
        Metaanalysis of filler materials in periapical surgery.
        Med Oral Patol Oral Cir Bucal. 2008; 13: E180-E185
        • Bryan E.B.
        • Woollard G.
        • Mitchell W.C.
        Nonsurgical repair of furcal perforations: a literature review.
        Gen Dent. 1999; 47: 274-278
        • Lee S.J.
        • Monsef M.
        • Torabinejad M.
        Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations.
        J Endod. 1993; 19: 541-544
        • Nakata T.T.
        • Bae K.S.
        • Baumgartner J.C.
        Perforation repair comparing mineral trioxide aggregate and amalgam using an anaerobic bacterial leakage model.
        J Endod. 1998; 24: 184-186
        • Daoudi M.F.
        • Saunders W.P.
        In vitro evaluation of furcal perforation repair using mineral trioxide aggregate or resin modified glass ionomer cement with and without the use of the operating microscope.
        J Endod. 2002; 28: 512-515
        • Weldon Jr., J.K.
        • Pashley D.H.
        • Loushine R.J.
        • Weller R.N.
        • Kimbrough W.F.
        Sealing ability of mineral trioxide aggregate and super-EBA when used as furcation repair materials: a longitudinal study.
        J Endod. 2002; 28: 467-470
        • Hardy I.
        • Liewehr F.R.
        • Joyce A.P.
        • Agee K.
        • Pashley D.H.
        Sealing ability of One-Up Bond and MTA with and without a secondary seal as furcation perforation repair materials.
        J Endod. 2004; 30: 658-661
        • Ferris D.M.
        • Baumgartner J.C.
        Perforation repair comparing two types of mineral trioxide aggregate.
        J Endod. 2004; 30: 422-424
        • Tsatsas D.V.
        • Meliou H.A.
        • Kerezoudis N.P.
        Sealing effectiveness of materials used in furcation perforation in vitro.
        Int Dent J. 2005; 55: 133-141
        • Hamad H.A.
        • Tordik P.A.
        • McClanahan S.B.
        Furcation perforation repair comparing gray and white MTA: a dye extraction study.
        J Endod. 2006; 32: 337-340
        • De-Deus G.
        • Petruccelli V.
        • Gurgel-Filho E.
        • Coutinho-Filho T.
        MTA versus Portland cement as repair material for furcal perforations: a laboratory study using a polymicrobial leakage model.
        Int Endod J. 2006; 39: 293-298
        • Hashem A.A.
        • Hassanien E.E.
        • ProRoot M.T.A.
        MTA-Angelus and IRM used to repair large furcation perforations: sealability study.
        J Endod. 2008; 34: 59-61
        • Zou L.
        • Liu J.
        • Yin S.
        • Li W.
        • Xie J.
        In vitro evaluation of the sealing ability of MTA used for the repair of furcation perforations with and without the use of an internal matrix.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 105: e61-e65
        • Shemesh H.
        • Souza E.M.
        • Wu M.K.
        • Wesselink P.R.
        Glucose reactivity with filling materials as a limitation for using the glucose leakage model.
        Int Endod J. 2008; 41: 869-872
        • Tselnik M.
        • Baumgartner J.C.
        • Marshall J.G.
        Bacterial leakage with mineral trioxide aggregate or a resin-modified glass ionomer used as a coronal barrier.
        J Endod. 2004; 30: 782-784
        • John A.D.
        • Webb T.D.
        • Imamura G.
        • Goodell G.G.
        Fluid flow evaluation of Fuji Triage and gray and white ProRoot mineral trioxide aggregate intraorifice barriers.
        J Endod. 2008; 34: 830-832
        • Barrieshi-Nusair K.M.
        • Hammad H.M.
        Intracoronal sealing comparison of mineral trioxide aggregate and glass ionomer.
        Quintessence Int. 2005; 36: 539-545
        • Jenkins S.
        • Kulild J.
        • Williams K.
        • Lyons W.
        • Lee C.
        Sealing ability of three materials in the orifice of root canal systems obturated with gutta-percha.
        J Endod. 2006; 32: 225-227
        • Zakizadeh P.
        • Marshall S.J.
        • Hoover C.I.
        • et al.
        A novel approach in assessment of coronal leakage of intraorifice barriers: a saliva leakage and micro-computed tomographic evaluation.
        J Endod. 2008; 34: 871-875
        • Stefopoulos S.
        • Tsatsas D.V.
        • Kerezoudis N.P.
        • Eliades G.
        Comparative in vitro study of the sealing efficiency of white vs grey ProRoot mineral trioxide aggregate formulas as apical barriers.
        Dent Traumatol. 2008; 24: 207-213
        • Martin R.L.
        • Monticelli F.
        • Brackett W.W.
        • et al.
        Sealing properties of mineral trioxide aggregate orthograde apical plugs and root fillings in an in vitro apexification model.
        J Endod. 2007; 33: 272-275
        • de Leimburg M.L.
        • Angeretti A.
        • Ceruti P.
        • Lendini M.
        • Pasqualini D.
        • Berutti E.
        MTA obturation of pulpless teeth with open apices: bacterial leakage as detected by polymerase chain reaction assay.
        J Endod. 2004; 30: 883-886
        • Al-Kahtani A.
        • Shostad S.
        • Schifferle R.
        • Bhambhani S.
        In-vitro evaluation of microleakage of an orthograde apical plug of mineral trioxide aggregate in permanent teeth with simulated immature apices.
        J Endod. 2005; 31: 117-119
        • Vizgirda P.J.
        • Liewehr F.R.
        • Patton W.R.
        • McPherson J.C.
        • Buxton T.B.
        A comparison of laterally condensed gutta-percha, thermoplasticized gutta-percha, and mineral trioxide aggregate as root canal filling materials.
        J Endod. 2004; 30: 103-106
        • Al-Hezaimi K.
        • Naghshbandi J.
        • Oglesby S.
        • Simon J.H.
        • Rotstein I.
        Human saliva penetration of root canals obturated with two types of mineral trioxide aggregate cements.
        J Endod. 2005; 31: 453-456
        • Bogen G.
        A comparison of laterally condensed gutta-percha, thermoplasticized gutta-percha, and mineral trioxide aggregate as root canal filling materials.
        J Endod. 2004; 30 (author reply 826): 826
        • Abuabara A.
        • Santos A.J.
        • Aguiar F.H.
        • Lovadino J.R.
        Evaluation of microleakage in human, bovine and swine enamels.
        Braz Oral Res. 2004; 18: 312-316
        • Camargo C.H.
        • Siviero M.
        • Camargo S.E.
        • de Oliveira S.H.
        • Carvalho C.A.
        • Valera M.C.
        Topographical, diametral, and quantitative analysis of dentin tubules in the root canals of human and bovine teeth.
        J Endod. 2007; 33: 4226
        • Camargo C.H.
        • Bernardineli N.
        • Valera M.C.
        • et al.
        Vehicle influence on calcium hydroxide pastes diffusion in human and bovine teeth.
        Dent Traumatol. 2006; 22: 302-306
        • Camargo S.E.
        • Valera M.C.
        • Camargo C.H.
        • Gasparoto Mancini M.N.
        • Menezes M.M.
        Penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique.
        J Endod. 2007; 33: 1074-1077
        • Torabinejad M.
        • Smith P.W.
        • Kettering J.D.
        • Pitt Ford T.R.
        Comparative investigation of marginal adaptation of mineral trioxide aggregate and other commonly used root-end filling materials.
        J Endod. 1995; 21: 295-299
        • Gondim E.
        • Zaia A.A.
        • Gomes B.P.
        • Ferraz C.C.
        • Teixeira F.B.
        • Souza-Filho F.J.
        Investigation of the marginal adaptation of root-end filling materials in root-end cavities prepared with ultrasonic tips.
        Int Endod J. 2003; 36: 491-499
        • Shipper G.
        • Grossman E.S.
        • Botha A.J.
        • Cleaton-Jones P.E.
        Marginal adaptation of mineral trioxide aggregate (MTA) compared with amalgam as a root-end filling material: a low-vacuum (LV) versus high-vacuum (HV) SEM study.
        Int Endod J. 2004; 37: 325-336
        • Peters C.I.
        • Peters O.A.
        Occlusal loading of EBA and MTA root-end fillings in a computer-controlled masticator: a scanning electron microscopic study.
        Int Endod J. 2002; 35: 22-29
        • Gandolfi M.G.
        • Sauro S.
        • Mannocci F.
        • et al.
        New tetrasilicate cements as retrograde filling material: an in vitro study on fluid penetration.
        J Endod. 2007; 33: 742-745
        • Xavier C.B.
        • Weismann R.
        • de Oliveira M.G.
        • Demarco F.F.
        • Pozza D.H.
        Root-end filling materials: apical microleakage and marginal adaptation.
        J Endod. 2005; 31: 539-542
        • de Souza E.B.
        • de Amorim C.V.
        • Marques J.L.
        Effect of diode laser irradiation on the apical sealing of MTA retrofillings.
        Braz Oral Res. 2006; 20: 231-234
        • Winik R.
        • Araki A.T.
        • Negrão J.A.
        • Bello-Silva M.S.
        • Lage-Marques J.L.
        Sealer penetration and marginal permeability after apicoectomy varying retrocavity preparation and retrofilling material.
        Braz Dent J. 2006; 17: 323-327
        • Shemesh H.
        To the editor: Hashem and Hassanien “ProRoot MTA, MTA-Angelus, and IRM used to repair large furcation perforations: sealability study”.
        J Endod. 2008; 34 (author reply 512–3): 512
        • Pelliccioni G.A.
        • Vellani C.P.
        • Gatto M.R.
        • Gandolfi M.G.
        • Marchetti C.
        • Prati C.
        Proroot mineral trioxide aggregate cement used as a retrograde filling without addition of water: an in vitro evaluation of its microleakage.
        J Endod. 2007; 33: 1082-1085
        • Shahi S.
        • Rahimi S.
        • Yavari H.R.
        • Shakouie S.
        • Nezafati S.
        • Abdolrahimi M.
        Sealing ability of white and gray mineral trioxide aggregate mixed with distilled water and 0.12% chlorhexidine gluconate when used as root-end filling materials.
        J Endod. 2007; 33: 1429-1432
        • Rahimi S.
        • Shahi S.
        • Lotfi M.
        • Yavari H.R.
        • Charehjoo M.E.
        Comparison of microleakage with three different thicknesses of mineral trioxide aggregate as root-end filling material.
        J Oral Sci. 2008; 50: 273-277
        • Camilleri J.
        • Montesin F.E.
        • Brady K.
        • Sweeney R.
        • Curtis R.V.
        • Ford T.R.
        The constitution of mineral trioxide aggregate.
        Dent Mater. 2005; 21: 297-303
        • Fridland M.
        • Rosado R.
        Mineral trioxide aggregate (MTA) solubility and porosity with different water-to-powder ratios.
        J Endod. 2003; 29: 814-817
        • De Bruyne M.A.
        • De Moor R.J.
        Influence of cracks on leakage and obturation efficiency of root-end filling materials after ultrasonic preparation: an in vitro evaluation.
        Quintessence Int. 2008; 39: 685-692
        • Nandini S.
        • Ballal S.
        • Kandaswamy D.
        Influence of glass-ionomer cement on the interface and setting reaction of mineral trioxide aggregate when used as a furcal repair material using laser Raman spectroscopic analysis.
        J Endod. 2007; 33: 167-172
        • Uyanik M.O.
        • Nagas E.
        • Sahin C.
        • Dagli F.
        • Cehreli Z.C.
        Effects of different irrigation regimens on the sealing properties of repaired furcal perforations.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107: e91-e95
        • Smith J.B.
        • Loushine R.J.
        • Weller R.N.
        • et al.
        Metrologic evaluation of the surface of white MTA after the use of two endodontic irrigants.
        J Endod. 2007; 33: 463-467
        • Rafter M.
        Apexification: a review.
        Dent Traumatol. 2005; 21: 1-8
        • Andreasen J.O.
        • Farik B.
        • Munksgaard E.C.
        Long-term calcium hydroxide as a root canal dressing may increase risk of root fracture.
        Dent Traumatol. 2002; 18: 134-137
        • Hatibović-Kofman S.
        • Raimundo L.
        • Zheng L.
        • Chong L.
        • Friedman M.
        • Andreasen J.O.
        Fracture resistance and histological findings of immature teeth treated with mineral trioxide aggregate.
        Dent Traumatol. 2008; 24: 272-276
        • Bramante C.M.
        • Luna-Cruz S.M.
        • Sipert C.R.
        • et al.
        Alveolar mucosa necrosis induced by utilisation of calcium hydroxide as root canal dressing.
        Int Dent J. 2008; 58: 81-85
        • Shabahang S.
        • Torabinejad M.
        Treatment of teeth with open apices using mineral trioxide aggregate.
        Pract Periodontics Aesthet Dent. 2000; 12: 315-320
        • Matt G.D.
        • Thorpe J.R.
        • Strother J.M.
        • McClanahan S.B.
        Comparative study of white and gray mineral trioxide aggregate (MTA) simulating a one- or two-step apical barrier technique.
        J Endod. 2004; 30: 876-879
        • Saghiri M.A.
        • Lotfi M.
        • Saghiri A.M.
        • Vosoughhosseini S.
        • Aeinehchi M.
        • Ranjkesh B.
        Scanning electron micrograph and surface hardness of mineral trioxide aggregate in the presence of alkaline pH.
        J Endod. 2009; 35: 706-710
        • Hachmeister D.R.
        • Schindler W.G.
        • Walker 3rd, W.A.
        • Thomas D.D.
        The sealing ability and retention characteristics of mineral trioxide aggregate in a model of apexification.
        J Endod. 2002; 28: 386-390
        • Sarkar N.K.
        • Caicedo R.
        • Ritwik P.
        • Moiseyeva R.
        • Kawashima I.
        Physicochemical basis of the biologic properties of mineral trioxide aggregate.
        J Endod. 2005; 31: 97-100
        • Bozeman T.B.
        • Lemon R.R.
        • Eleazer P.D.
        Elemental analysis of crystal precipitate from gray and white MTA.
        J Endod. 2006; 32: 425-428
        • Lawley G.R.
        • Schindler W.G.
        • Walker 3rd, W.A.
        • Kolodrubetz D.
        Evaluation of ultrasonically placed MTA and fracture resistance with intracanal composite resin in a model of apexification.
        J Endod. 2004; 30: 167-172
        • Kim U.S.
        • Shin S.J.
        • Chang S.W.
        • Yoo H.M.
        • Oh T.S.
        • Park D.S.
        In vitro evaluation of bacterial leakage resistance of an ultrasonically placed mineral trioxide aggregate orthograde apical plug in teeth with wide open apexes: a preliminary study.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107: e52-e56
        • Aminoshariae A.
        • Hartwell G.R.
        • Moon P.C.
        Placement of mineral trioxide aggregate using two different techniques.
        J Endod. 2003; 29: 679-682
        • Chogle S.
        • Mickel A.K.
        • Chan D.M.
        • Huffaker K.
        • Jones J.J.
        Intracanal assessment of mineral trioxide aggregate setting and sealing properties.
        Gen Dent. 2007; 55: 306-311
        • Bidar M.
        • Moradi S.
        • Jafarzadeh H.
        • Bidad S.
        Comparative SEM study of the marginal adaptation of white and grey MTA and Portland cement.
        Aust Endod J. 2007; 33: 2-6
        • Costa A.T.
        • Konrath F.
        • Dedavid B.
        • Weber J.B.
        • de Oliveira M.G.
        Marginal adaptation of root-end filling materials: an in vitro study with teeth and replicas.
        J Contemp Dent Pract. 2009; 10: 75-82
        • de Martins G.R.
        • Carvalho C.A.
        • Valera M.C.
        • de Oliveira L.D.
        • Buso L.
        • Carvalho A.S.
        Sealing ability of castor oil polymer as a root-end filling material.
        J Appl Oral Sci. 2009; 17: 220-223
        • Lolayekar N.
        • Baht S.S.
        • Hegde S.
        Sealing ability of ProRoot MTA and MTA-Angelus simulating a one-Step apical barrier technique- an in vitro study.
        J Clin Pediatr Dent. 2009; 33: 305-310
        • Bortoluzzi E.A.
        • Broon N.J.
        • Bramante C.M.
        • Garcia R.B.
        • de Moraes I.G.
        • Bernardineli N.
        Sealing ability of MTA and radiopaque Portland cement with or without calcium chloride for root-end filling.
        J Endod. 2006; 32: 897-900
        • De-Deus G.
        • Reis C.
        • Brandão C.
        • Fidel S.
        • Fidel R.A.
        The ability of Portland cement, MTA, and MTA Bio to prevent through-and-through fluid movement in repaired furcal perforations.
        J Endod. 2007; 33: 1374-1377
        • Reyes-Carmona J.F.
        • Felippe M.S.
        • Felippe W.T.
        Biomineralization ability and interaction of mineral trioxide aggregate and white portland cement with dentin in a phosphate-containing fluid.
        J Endod. 2009; 35: 731-736
        • Budig C.G.
        • Eleazer P.D.
        In vitro comparison of the setting of dry ProRoot MTA by moisture absorbed through the root.
        J Endod. 2008; 34: 712-714
        • Hong S.T.
        • Bae K.S.
        • Baek S.H.
        • Kum K.Y.
        • Lee W.
        Microleakage of accelerated mineral trioxide aggregate and Portland cement in an in vitro apexification model.
        J Endod. 2008; 34: 56-58
        • Holt D.M.
        • Watts J.D.
        • Beeson T.J.
        • Kirkpatrick T.C.
        • Rutledge R.E.
        The anti-microbial effect against enterococcus faecalis and the compressive strength of two types of mineral trioxide aggregate mixed with sterile water or 2% chlorhexidine liquid.
        J Endod. 2007; 33: 844-847
        • Weller R.N.
        • Tay K.C.
        • Garrett L.V.
        • et al.
        Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid.
        Int Endod J. 2008; 41: 977-986
        • Huffman B.P.
        • Mai S.
        • Pinna L.
        • et al.
        Dislocation resistance of ProRoot Endo Sealer, a calcium silicate-based root canal sealer, from radicular dentine.
        Int Endod J. 2009; 42: 34-46
        • Shahravan A.
        • Haghdoost A.A.
        • Adl A.R.
        • Rahimi H.
        • Shadifar F.
        Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis.
        J Endod. 2007; 33: 96-105
        • Yildirim T.
        • Oruçoğlu H.
        • Cobankara F.K.
        Long-term evaluation of the influence of smear layer on the apical sealing ability of MTA.
        J Endod. 2008; 34: 1537-1540
        • Lee Y.L.
        • Lin F.H.
        • Wang W.H.
        • Ritchie H.H.
        • Lan W.H.
        • Lin C.P.
        Effects of EDTA on the hydration mechanism of mineral trioxide aggregate.
        J Dent Res. 2007; 86: 534-538
        • Jacobovitz M.
        • Vianna M.E.
        • Pandolfelli V.C.
        • Oliveira I.R.
        • Rossetto H.L.
        • Gomes B.P.
        Root canal filling with cements based on mineral aggregates: an in vitro analysis of bacterial microleakage.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108: 140-144
        • Orosco F.A.
        • Bramante C.M.
        • Garcia R.B.
        • Bernadineli N.
        • Moraes I.G.
        Sealing ability of grar MTA AngelusTM, CPM TM and MBPc used as apical plugs.
        J Appl Oral Sci. 2008; 16: 50-54
        • Camilleri J.
        • Montesin F.E.
        • Di Silvio L.
        • Pitt Ford T.R.
        The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use.
        Int Endod J. 2005; 38: 834-842
        • Camilleri J.
        • Montesin F.E.
        • Papaioannou S.
        • McDonald F.
        • Pitt Ford T.R.
        Biocompatibility of two commercial forms of mineral trioxide aggregate.
        Int Endod J. 2004; 37: 699-704
        • Saidon J.
        • He J.
        • Zhu Q.
        • Safavi K.
        • Spångberg L.S.
        Cell and tissue reactions to mineral trioxide aggregate and Portland cement.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95: 483-489
        • De Deus G.
        • Ximenes R.
        • Gurgel-Filho E.D.
        • Plotkowski M.C.
        • Coutinho-Filho T.
        Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells.
        Int Endod J. 2005; 38: 604-609
        • Kim E.C.
        • Lee B.C.
        • Chang H.S.
        • Lee W.
        • Hong C.U.
        • Min K.S.
        Evaluation of the radiopacity and cytotoxicity of Portland cements containing bismuth oxide.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 105: e54-e57
        • Bodrumlu E.
        Biocompatibility of retrograde root filling materials: a review.
        Aust Endod J. 2008; 34: 30-35
        • Kettering J.D.
        • Torabinejad M.
        Investigation of mutagenicity of mineral trioxide aggregate and other commonly used root-end filling materials.
        J Endod. 1995; 21: 537-542
        • Asrari M.
        • Lobner D.
        In vitro neurotoxic evaluation of root-end-filling materials.
        J Endod. 2003; 29: 743-746
        • Abbasipour F.
        • Rastqar A.
        • Bakhtiar H.
        • Khalilkhani H.
        • Aeinehchi M.
        • Janahmadi M.
        The nociceptive and anti-nociceptive effects of white mineral trioxide aggregate.
        Int Endod J. 2009; 42: 794-801
        • Masuda Y.M.
        • Wang X.
        • Hossain M.
        • et al.
        Evaluation of biocompatibility of mineral trioxide aggregate with an improved rabbit ear chamber.
        J Oral Rehabil. 2005; 32: 145-150
        • Tunca Y.M.
        • Aydin C.
        • Ozen T.
        • Seyrek M.
        • Ulusoy H.B.
        • Yildiz O.
        The effect of mineral trioxide aggregate on the contractility of the rat thoracic aorta.
        J Endod. 2007; 33: 823-826
        • Torabinejad M.
        • Hong C.U.
        • Pitt Ford T.R.
        • Kettering J.D.
        Cytotoxicity of four root end filling materials.
        J Endod. 1995; 21: 489-492
        • Osorio R.M.
        • Hefti A.
        • Vertucci F.J.
        • Shawley A.L.
        Cytotoxicity of endodontic materials.
        J Endod. 1998; 24: 91-96
        • Keiser K.
        • Johnson C.C.
        • Tipton D.A.
        Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts.
        J Endod. 2000; 26: 288-291
        • Zhu Q.
        • Haglund R.
        • Safavi K.E.
        • Spangberg L.S.
        Adhesion of human osteoblasts on root-end filling materials.
        J Endod. 2000; 26: 404-406
        • Thomson T.S.
        • Berry J.E.
        • Somerman M.J.
        • Kirkwood K.L.
        Cementoblasts maintain expression of osteocalcin in the presence of mineral trioxide aggregate.
        J Endod. 2003; 29: 407-412
        • Pérez A.L.
        • Spears R.
        • Gutmann J.L.
        • Opperman L.A.
        Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot MTA and White MTA.
        Int Endod J. 2003; 36: 564-570
        • Pistorius A.
        • Willershausen B.
        • Briseño Marroquin B.
        Effect of apical root-end filling materials on gingival fibroblasts.
        Int Endod J. 2003; 36: 610-615
        • Fayad M.I.
        • Hawkinson R.
        • Daniel J.
        • Hao J.
        The effect of CO2 laser irradiation on PDL cell attachment to resected root surfaces.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97: 518-523
        • Bonson S.
        • Jeansonne B.G.
        • Lallier T.E.
        Root-end filling materials alter fibroblast differentiation.
        J Dent Res. 2004; 83: 408-413
        • Balto H.A.
        Attachment and morphological behavior of human periodontal ligament fibroblasts to mineral trioxide aggregate: a scanning electron microscope study.
        J Endod. 2004; 30: 25-29
        • Pelliccioni G.A.
        • Ciapetti G.
        • Cenni E.
        • et al.
        Evaluation of osteoblast-like cell response to Proroot MTA (mineral trioxide aggregate) cement.
        J Mater Sci Mater Med. 2004; 15: 167-173
        • Koulaouzidou E.A.
        • Papazisis K.T.
        • Economides N.A.
        • Beltes P.
        • Kortsaris A.H.
        Antiproliferative effect of mineral trioxide aggregate, zinc oxide-eugenol cement, and glass-ionomer cement against three fibroblastic cell lines.
        J Endod. 2005; 31: 44-46
        • Nakayama A.
        • Ogiso B.
        • Tanabe N.
        • Takeichi O.
        • Matsuzaka K.
        • Inoue T.
        Behaviour of bone marrow osteoblast-like cells on mineral trioxide aggregate: morphology and expression of type I collagen and bone-related protein mRNAs.
        Int Endod J. 2005; 38: 203-210
        • Huang T.H.
        • Yang C.C.
        • Ding S.J.
        • Yan M.
        • Chou M.Y.
        • Kao C.T.
        Biocompatibility of human osteosarcoma cells to root end filling materials.
        J Biomed Mater Res B Appl Biomater. 2005; 72: 140-145
        • Moghaddame-Jafari S.
        • Mantellini M.G.
        • Botero T.M.
        • McDonald N.J.
        • Nör J.E.
        Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro.
        J Endod. 2005; 31: 387-391
        • Vajrabhaya L.O.
        • Korsuwannawong S.
        • Jantarat J.
        • Korre S.
        Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102: e48-e50
        • Al-Rabeah E.
        • Perinpanayagam H.
        • MacFarland D.
        Human alveolar bone cells interact with ProRoot and tooth-colored MTA.
        J Endod. 2006; 32: 872-875
        • Kao C.T.
        • Tsai C.H.
        • Huang T.H.
        Tissue and cell reactions to implanted root-end filling materials.
        J Mater Sci Mater Med. 2006; 17: 841-847
        • Takita T.
        • Hayashi M.
        • Takeichi O.
        • et al.
        Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells.
        Int Endod J. 2006; 39: 415-422
        • Melegari K.K.
        • Botero T.M.
        • Holland G.R.
        Prostaglandin E production and viability of cells cultured in contact with freshly mixed endodontic materials.
        Int Endod J. 2006; 39: 357-362
        • Oviir T.
        • Pagoria D.
        • Ibarra G.
        • Geurtsen W.
        Effects of gray and white mineral trioxide aggregate on the proliferation of oral keratinocytes and cementoblasts.
        J Endod. 2006; 32: 210-213
        • Souza N.J.
        • Justo G.Z.
        • Oliveira C.R.
        • Haun M.
        • Bincoletto C.
        Cytotoxicity of materials used in perforation repair tested using the V79 fibroblast cell line and the granulocyte-macrophage progenitor cells.
        Int Endod J. 2006; 39: 40-47
        • Yoshimine Y.
        • Ono M.
        • Akamine A.
        In vitro comparison of the biocompatibility of mineral trioxide aggregate, 4META/MMA-TBB resin, and intermediate restorative material as root-end-filling materials.
        J Endod. 2007; 33: 1066-1069
        • Gorduysus M.
        • Avcu N.
        • Gorduysus O.
        • et al.
        Cytotoxic effects of four different endodontic materials in human periodontal ligament fibroblasts.
        J Endod. 2007; 33: 1450-1454
        • Tani-Ishii N.
        • Hamada N.
        • Watanabe K.
        • Tujimoto Y.
        • Teranaka T.
        • Umemoto T.
        Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide.
        J Endod. 2007; 33: 836-839
        • Guven G.
        • Cehreli Z.C.
        • Ural A.
        • Serdar M.A.
        • Basak F.
        Effect of mineral trioxide aggregate cements on transforming growth factor beta1 and bone morphogenetic protein production by human fibroblasts in vitro.
        J Endod. 2007; 33: 447-450
        • Coon D.
        • Gulati A.
        • Cowan C.
        • He J.
        The role of cyclooxygenase-2 (COX-2) in inflammatory bone resorption.
        J Endod. 2007; 33: 432-436
        • de Souza Costa C.A.
        • Duarte P.T.
        • de Souza P.P.
        • Giro E.M.
        • Hebling J.
        Cytotoxic effects and pulpal response caused by a mineral trioxide aggregate formulation and calcium hydroxide.
        Am J Dent. 2008; 21: 255-261
        • Laurent P.
        • Camps J.
        • De Méo M.
        • Déjou J.
        • About I.
        Induction of specific cell responses to a Ca(3)SiO(5)-based posterior restorative material.
        Dent Mater. 2008; 24: 1486-1494
        • Yasuda Y.
        • Ogawa M.
        • Arakawa T.
        • Kadowaki T.
        • Saito T.
        The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study.
        J Endod. 2008; 34: 1057-1060
        • Koh E.T.
        • McDonald F.
        • Pitt Ford T.R.
        • Torabinejad M.
        Cellular response to Mineral Trioxide Aggregate.
        J Endod. 1998; 24: 543-547
        • Camp M.A.
        • Jeansonne B.G.
        • Lallier T.
        Adhesion of human fibroblasts to root-end-filling materials.
        J Endod. 2003; 29: 602-607
        • Haglund R.
        • He J.
        • Jarvis J.
        • Safavi K.E.
        • Spångberg L.S.
        • Zhu Q.
        Effects of root-end filling materials on fibroblasts and macrophages in vitro.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95: 739-745
        • Asgary S.
        • Parirokh M.
        • Eghbal M.J.
        • Brink F.
        Chemical differences between white and gray mineral trioxide aggregate.
        J Endod. 2005; 31: 101-103
        • Asgary S.
        • Parirokh M.
        • Eghbal M.J.
        • Stowe S.
        • Brink F.
        A qualitative X-ray analysis of white and grey mineral trioxide aggregate using compositional imaging.
        J Mater Sci Mater Med. 2006; 17: 187-191
        • Hakki S.S.
        • Bozkurt S.B.
        • Hakki E.E.
        • Belli S.
        Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts.
        J Endod. 2009; 35: 513-519
        • Sepet E.
        • Pinar A.
        • Ilhan B.
        • Ulukapi I.
        • Bilir A.
        • Tuna S.
        Cytotoxic effects of calcium hydroxide and mineral trioxide aggregate on 3t3 fibroblast cell line in vitro.
        Quintessence Int. 2009; 40: e55-e61
        • Chen C.L.
        • Huang T.H.
        • Ding S.J.
        • Shie M.Y.
        • Kao C.T.
        Comparison of calcium and silicate cement and mineral trioxide aggregate biologic effects and bone markers expression in MG63 cells.
        J Endod. 2009; 35: 682-685
        • Ribeiro D.A.
        • Matsumoto M.A.
        • Duarte M.A.
        • Marques M.E.
        • Salvadori D.M.
        In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate.
        Braz Oral Res. 2005; 19: 183-187
        • Ribeiro D.A.
        • Matsumoto M.A.
        • Duarte M.A.
        • Marques M.E.
        • Salvadori D.M.
        Ex vivo biocompatibility tests of regular and white forms of mineral trioxide aggregate.
        Int Endod J. 2006; 39: 26-30
        • da Silva G.N.
        • Braz M.G.
        • de Camargo E.A.
        • Salvadori D.M.
        • Ribeiro D.A.
        Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102: e50-e54
        • Ribeiro D.A.
        • Sugui M.M.
        • Matsumoto M.A.
        • Duarte M.A.
        • Marques M.E.
        • Salvadori D.M.
        Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 101: 258-261
        • Braz M.G.
        • Camargo E.A.
        • Salvadori D.M.
        • Marques M.E.
        • Ribeiro D.A.
        Evaluation of genetic damage in human peripheral lymphocytes exposed to mineral trioxide aggregate and Portland cements.
        J Oral Rehabil. 2006; 33: 234-239
        • De Deus G.
        • Ximenes R.
        • Gurgel-Filho E.D.
        • Plotkowski M.C.
        • Coutinho-Filho T.
        Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells.
        Int Endod J. 2005; 38: 604-609
        • Koulaouzidou E.A.
        • Economides N.
        • Beltes P.
        • Geromichalos G.
        • Papazisis K.
        In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus.
        J Oral Sci. 2008; 50: 397-402
        • Miranda R.B.
        • Fidel S.R.
        • Boller M.A.
        L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay.
        Braz Dent J. 2009; 20: 22-26
        • de Menezes J.V.
        • Takamori E.R.
        • Bijella M.F.
        • Granjeiro J.M.
        In vitro toxicity of MTA compared with other primary teeth pulpotomy agents.
        J Clin Pediatr Dent. 2009; 33: 217-221
        • Camargo S.E.
        • Camargo C.H.
        • Hiller K.A.
        • Rode S.M.
        • Schweikl H.
        • Schmalz G.
        Cytotoxicity and genotoxicity of pulp capping materials in two cell lines.
        Int Endod J. 2009; 42: 227-237
        • Kogan P.
        • He J.
        • Glickman G.N.
        • Watanabe I.
        The effects of various additives on setting properties of MTA.
        J Endod. 2006; 32: 569-572
        • Wiltbank K.B.
        • Schwartz S.A.
        • Schindler W.G.
        Effect of selected accelerants on the physical properties of mineral trioxide aggregate and Portland cement.
        J Endod. 2007; 33: 1235-1238
        • Ber B.S.
        • Hatton J.F.
        • Stewart G.P.
        Chemical modification of proroot mta to improve handling characteristics and decrease setting time.
        J Endod. 2007; 33: 1231-1234
        • Huang T.H.
        • Shie M.Y.
        • Kao C.T.
        • Ding S.J.
        The effect of setting accelerator on properties of mineral trioxide aggregate.
        J Endod. 2008; 34: 590-593
        • Ding S.J.
        • Kao C.T.
        • Shie M.Y.
        • Hung Jr., C.
        • Huang T.H.
        The physical and cytological properties of white MTA mixed with Na2HPO4 as an accelerant.
        J Endod. 2008; 34: 748-751
        • Stowe T.J.
        • Sedgley C.M.
        • Stowe B.
        • Fenno J.C.
        The effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored ProRoot mineral trioxide aggregate.
        J Endod. 2004; 30: 429-431
        • Hong S.T.
        • Bae K.S.
        • Baek S.H.
        • Kum K.Y.
        • Lee W.
        Microleakage of accelerated mineral trioxide aggregate and Portland cement in an in vitro apexification model.
        J Endod. 2008; 34: 56-58
        • Ding S.J.
        • Kao C.T.
        • Shie M.Y.
        • Hung Jr., C.
        • Huang T.H.
        The physical and cytological properties of white MTA mixed with Na2HPO4 as an accelerant.
        J Endod. 2008; 34: 748-751
        • Jafarnia B.
        • Jiang J.
        • He J.
        • Wang Y.H.
        • Safavi K.E.
        • Zhu Q.
        Evaluation of cytotoxicity of MTA employing various additives.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107: 739-744
        • Min K.S.
        • Yang S.H.
        • Kim E.C.
        The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells.
        J Endod. 2009; 35: 847-851
        • Hernandez E.P.
        • Botero T.M.
        • Mantellini M.G.
        • McDonald N.J.
        • Nör J.E.
        Effect of ProRoot MTA mixed with chlorhexidine on apoptosis and cell cycle of fibroblasts and macrophages in vitro.
        Int Endod J. 2005; 38: 137-143
        • Karimjee C.K.
        • Koka S.
        • Rallis D.M.
        • Gound T.G.
        Cellular toxicity of mineral trioxide aggregate mixed with an alternative delivery vehicle.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102: e115-e120
        • Abdullah D.
        • Ford T.R.
        • Papaioannou S.
        • Nicholson J.
        • McDonald F.
        An evaluation of accelerated Portland cement as a restorative material.
        Biomaterials. 2002; 23: 4001-4010
        • Koh E.T.
        • Torabinejad M.
        • Pitt Ford T.R.
        • Brady K.
        • McDonald F.
        Mineral trioxide aggregate stimulates a biological response in human osteoblasts.
        J Biomed Mater Res. 1997; 37: 432-439
        • Mitchell P.J.
        • Pitt Ford T.R.
        • Torabinejad M.
        • McDonald F.
        Osteoblast biocompatibility of mineral trioxide aggregate.
        Biomaterials. 1999; 20: 167-173
        • Huang T.H.
        • Ding S.J.
        • Hsu T.C.
        • Kao C.T.
        Effects of mineral trioxide aggregate (MTA) extracts on mitogen-activated protein kinase activity in human osteosarcoma cell line (U2OS).
        Biomaterials. 2003; 24: 3909-3913
        • Huang T.H.
        • Yang C.C.
        • Ding S.J.
        • Yeng M.
        • Kao C.T.
        • Chou M.Y.
        Inflammatory cytokines reaction elicited by root-end filling materials.
        J Biomed Mater Res B Appl Biomater. 2005; 73: 123-128
        • Rezende T.M.
        • Vargas D.L.
        • Cardoso F.P.
        • Sobrinho A.P.
        • Vieira L.Q.
        Effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages.
        Int Endod J. 2005; 38: 896-903
        • Tomson P.L.
        • Grover L.M.
        • Lumley P.J.
        • Sloan A.J.
        • Smith A.J.
        • Cooper P.R.
        Dissolution of bio-active dentine matrix components by mineral trioxide aggregate.
        J Dent. 2007; 35: 636-642
        • Rezende T.M.
        • Vieira L.Q.
        • Cardoso F.P.
        • et al.
        The effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by M1 and M2 macrophages.
        Int Endod J. 2007; 40: 603-611
        • Simon S.
        • Cooper P.
        • Smith A.
        • Picard B.
        • Ifi C.N.
        • Berdal A.
        Evaluation of a new laboratory model for pulp healing: preliminary study.
        Int Endod J. 2008; 41: 781-790
        • Deller-Quinn M.
        • Perinpanayagam H.
        Osteoblast expression of cytokines is altered on MTA surfaces.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108: 302-307
        • Minamikawa H.
        • Deyama Y.
        • Nakamura K.
        • et al.
        Effect of mineral trioxide aggregate on rat clonal dental pulp cells: expression of cyclooxygenase-2 mRNA and inflammation-related protein via nuclear factor kappa B signaling system.
        J Endod. 2009; 35: 843-846
        • Andelin W.E.
        • Shabahang S.
        • Wright K.
        • Torabinejad M.
        Identification of hard tissue after experimental pulp capping using dentin sialoprotein (DSP) as a marker.
        J Endod. 2003; 29: 646-650
        • Perinpanayagam H.
        • Al-Rabeah E.
        Osteoblasts interact with MTA surfaces and express Runx2.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107: 590-596
        • Masuda-Murakami Y.
        • Kobayashi M.
        • Wang X.
        • et al.
        Effectsof mineral trioxide aggregate on the differentiation of rat dental pulp cells.
        Acta Histochem. 2009 Jun 26; ([Epub ahead of print])
        • Ham K.A.
        • Witherspoon D.E.
        • Gutmann J.L.
        • Ravindranath S.
        • Gait T.C.
        • Opperman L.A.
        Preliminary evaluation of BMP-2 expression and histological characteristics during apexification with calcium hydroxide and mineral trioxide aggregate.
        J Endod. 2005; 31: 275-279
        • Kuratate M.
        • Yoshiba K.
        • Shigetani Y.
        • Yoshiba N.
        • Ohshima H.
        • Okiji T.
        Immunohistochemical analysis of nestin, osteopontin, and proliferating cells in the reparative process of exposed dental pulp capped with mineral trioxide aggregate.
        J Endod. 2008; 34: 970-974
        • He W.X.
        • Niu Z.Y.
        • Zhao S.L.
        • Jin W.L.
        • Gao J.
        • Smith A.J.
        TGF-beta activated Smad signalling leads to a Smad3-mediated down-regulation of DSPP in an odontoblast cell line.
        Arch Oral Biol. 2004; 49: 911-918
        • Guichard J.
        • Varet B.
        • Hermine O.
        Transforming growth factor inhibits erythropoiesis by blocking proliferation and accelerating differentiation of erythroid progenitors.
        Exp Hematol. 2000; 28: 885-894
        • Zermati Y.
        • Fichelson S.
        • Valensi F.
        • et al.
        Transforming growth factor inhibits erythropoiesis by blocking proliferation and accelerating differentiation of erythroid progenitors.
        Exp Hematol. 2000; 28: 885-894
        • He W.X.
        • Niu Z.Y.
        • Zhao S.L.
        • Smith A.J.
        Smad protein mediated transforming growth factor beta1 induction of apoptosis in the MDPC-23 odontoblast-like cell line.
        Arch Oral Biol. 2005; 50: 929-936
        • Hunter G.K.
        • Hauschka P.V.
        • Poole A.R.
        • Rosenberg L.C.
        • Goldberg H.A.
        Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins.
        Biochem J. 1996; 317: 59-64
        • Pampena D.A.
        • Robertson K.A.
        • Litvinova O.
        • Lajoie G.
        • Goldberg H.A.
        • Hunter G.K.
        Inhibition of hydroxyapatite formation by osteopontin phosphopeptides.
        Biochem J. 2004; 378: 1083-1087
        • Min K.S.
        • Park H.J.
        • Lee S.K.
        • et al.
        Effect of mineral trioxide aggregate on dentin bridge formation and expression of dentin sialoprotein and heme oxygenase-1 in human dental pulp.
        J Endod. 2008; 34: 666-670
        • Gomes A.C.
        • Filho J.E.
        • de Oliveira S.H.
        MTA-induced neutrophil recruitment: a mechanism dependent on IL-1beta, MIP-2, and LTB4.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 106: 450-456
        • Brewer J.M.
        (How) do aluminium adjuvants work?.
        Immunol Lett. 2006; 102: 10-15
        • Rezende T.M.
        • Vieira L.Q.
        • Sobrinho A.P.
        • Oliveira R.R.
        • Taubman M.A.
        • Kawai T.
        The influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice.
        J Endod. 2008; 34: 1066-1071
        • Silva M.J.
        • Vieira L.Q.
        • Sobrinho A.P.
        The effects of mineral trioxide aggregates on cytokine production by mouse pulp tissue.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 105: e70-e76
        • Holland R.
        • de Souza V.
        • Nery M.J.
        • Otoboni Filho J.A.
        • Bernabé P.F.
        • Dezan Júnior E.
        Reaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggregate or calcium hydroxide.
        J Endod. 1999; 25: 161-166
        • Moretton T.R.
        • Brown Jr., C.E.
        • Legan J.J.
        • Kafrawy A.H.
        Tissue reactions after subcutaneous and intraosseous implantation of mineral trioxide aggregate and ethoxybenzoic acid cement.
        J Biomed Mater Res. 2000; 52: 528-533
        • Holland R.
        • de Souza V.
        • Nery M.J.
        • et al.
        Reaction of rat connective tissue to implanted dentin tube filled with mineral trioxide aggregate, Portland cement or calcium hydroxide.
        Braz Dent J. 2001; 12: 3-8
        • Holland R.
        • de Souza V.
        • Nery M.J.
        • et al.
        Calcium salts deposition in rat connective tissue after the implantation of calcium hydroxide-containing sealers.
        J Endod. 2002; 28: 173-176
        • Holland R.
        • Souza V.
        • Nery M.J.
        • et al.
        Reaction of rat connective tissue to implanted dentin tubes filled with a white mineral trioxide aggregate.
        Braz Dent J. 2002; 13: 23-26
        • Yaltirik M.
        • Ozbas H.
        • Bilgic B.
        • Issever H.
        Reactions of connective tissue to mineral trioxide aggregate and amalgam.
        J Endod. 2004; 30: 95-99
        • Modaresi J.
        • Yavari S.A.
        • Dianat S.O.
        • Shahrabi S.
        A comparison of tissue reaction to MTA and an experimental root-end restorative material in rats.
        Aust Endod J. 2005; 31: 69-72
        • Sumer M.
        • Muglali M.
        • Bodrumlu E.
        • Guvenc T.
        Reactions of connective tissue to amalgam, intermediate restorative material, mineral trioxide aggregate, and mineral trioxide aggregate mixed with chlorhexidine.
        J Endod. 2006; 32: 1094-1096
        • Shahi S.
        • Rahimi S.
        • Lotfi M.
        • Yavari H.
        • Gaderian A.
        A comparative study of the biocompatibility of three root-end filling materials in rat connective tissue.
        J Endod. 2006; 32: 776-780
        • Vosoughhosseini S.
        • Lotfi M.
        • Shahi S.
        • et al.
        Influence of white versus gray mineral trioxide aggregate on inflammatory cells.
        J Endod. 2008; 34: 715-717
        • Prescott R.S.
        • Alsanea R.
        • Fayad M.I.
        • et al.
        In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice.
        J Endod. 2008; 34: 421-426
        • Hwang Y.C.
        • Lee S.H.
        • Hwang I.N.
        • et al.
        Chemical composition, radiopacity, and biocompatibility of Portland cement with bismuth oxide.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107: e96-102
        • Laliz E.M.
        • Esain M.L.
        • Kokubu G.A.
        • Willis J.
        • Chaves C.
        • Grana D.R.
        Rat Subcutaneous tissue response to modified Portland cement, a new mineral trioxide aggregate.
        Braz Dent J. 2009; 20: 112-117
        • Lotfi M.
        • Vosoughhosseini S.
        • Saghiri M.A.
        • Mesgariabbasi M.
        • Ranjkesh B.
        Effect of white mineral trioxide aggregate mixed with disodium hydrogen phosphate on inflammatory cells.
        J Endod. 2009; 35: 703-705
        • Gomes-Filho J.E.
        • Watanabe S.
        • Bernabé P.F.
        • de Moraes Costa M.T.
        A mineral trioxide aggregate sealer stimulated mineralization.
        J Endod. 2009; 35: 256-260
        • Torabinejad M.
        • Hong C.U.
        • Pitt Ford T.R.
        • Kaiyawasam S.P.
        Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report.
        J Endod. 1995; 21: 569-571
        • Torabinejad M.
        • Ford T.R.
        • Abedi H.R.
        • Kariyawasam S.P.
        • Tang H.M.
        Tissue reaction to implanted root-end filling materials in the tibia and mandible of guinea pigs.
        J Endod. 1998; 24: 468-471
        • Sousa C.J.
        • Loyola A.M.
        • Versiani M.A.
        • Biffi J.C.
        • Oliveira R.P.
        • Pascon E.A.
        A comparative histological evaluation of the biocompatibility of materials used in apical surgery.
        Int Endod J. 2004; 37: 738-748
        • Cintra L.T.
        • de Moraes I.G.
        • Estrada B.P.
        • et al.
        Evaluation of the tissue response to MTA and MBPC: microscopic analysis of implants in alveolar bone of rats.
        J Endod. 2006; 32: 556-559