Abstract
Introduction
We have previously shown that the p38 gene is highly expressed in odontoblasts during
active primary dentinogenesis, but is drastically down-regulated as cells become quiescent
in secondary dentinogenesis. Based on these observations, we hypothesized that p38
expression might be upregulated, and the protein activated by phosphorylation, when
odontoblasts are stimulated such as during tertiary reactionary dentinogenesis.
Methods
We stimulated immortalized, odontoblast-like MDPC-23 cells, alone or in combination,
with heat-inactivated Streptococcus mutans, EDTA-extracted dentine matrix proteins (DMPs), or growth factors, including transforming
growth factor (TGF)-β1, tumor necrosis factor-α (TNF-α), and adrenomedullin (ADM).
We used ELISA to measure the resulting phosphorylation of the p38 protein, as well
as its degree of nuclear translocation.
Results
Our results suggest that the p38-MAPKinase pathway is activated during odontoblast
stimulation in tertiary dentinogenesis by both p38 phosphorylation and enhanced nuclear
translocation.
Conclusions
Data indicate that odontoblast behaviour therefore potentially recapitulates that
during active primary dentinogenesis.
Key Words
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Article info
Publication history
Published online: December 11, 2009
Identification
Copyright
© 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
