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Basic Research| Volume 36, ISSUE 2, P282-285, February 2010

Final Rinse Optimization: Influence of Different Agitation Protocols

Published:December 07, 2009DOI:https://doi.org/10.1016/j.joen.2009.10.004

      Abstract

      Introduction

      This study examined the effect of different root canal irrigant agitation protocols in the penetration of an endodontic irrigant into dentinal tubules.

      Methods

      Fifty-six human single-rooted teeth were shaped with nickel-titanium instruments, and a final rinse of 5% sodium hypochlorite labeled with 0.2% alizarin red was performed. Specimens were assigned to 7 groups (N = 8) and submitted to the following rinse activation protocols: no agitation (control group), K-File or gutta-percha agitation, or different sonic (EndoActivator [Advanced Endodontics, Santa Barbara, CA] and Plastic Endo, Lincolnshire, IL) and ultrasonic (Satelec [Acteongroup, Merignac, France] and EMS, Nyon, Switzerland) agitations. Specimens were sectioned at 1, 3, and 5 mm from the apex in 1-mm-thick slabs, ground, and prepared for fluorescence microscopy at 100× with a wavelength of 450 milliseconds. Irrigant penetration into dentinal tubules was analyzed by using Kruskal-Wallis analysis of variance followed by post-hoc comparisons.

      Results

      Groups were ranked in the following order: control = K-file = gutta-percha < EndoActivator = Plastic Endo < Satelec = EMS. At 1 mm from the apex, the highest score was found for the EMS group compared with the control, K-file, gutta-percha, EndoActivator, and Plastic Endo groups, whereas no difference was found with the Satelec group.

      Conclusion

      The results support the use of an ultrasonic agitation to increase the effectiveness of the final rinse procedure in the apical third of the canal walls.

      Key Words

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      References

        • Grossman L.I.
        • Meinmamn B.W.
        Solution of pulp tissue by chemical agents.
        J Am Dent Assoc. 1941; 8: 223-225
        • Harrison J.
        Irrigation of the root canal system.
        Dent Clin North Am. 1984; 28: 797-798
        • Peters O.A.
        Current challenges and concepts in the preparation of root canal systems: a review.
        J Endod. 2004; 30: 559-567
        • Zehnder M.
        Root canal irrigants review.
        J Endod. 2006; 32: 389-398
        • Naenni N.
        • Thoma K.
        • Zehnder M.
        Soft tissue dissolution capacity of currently used and potential endodontic irrigants.
        J Endod. 2004; 30: 785-787
        • Haikel Y.
        • Gorce F.
        • Allemann C.
        • et al.
        In vitro efficiency of endodontic irrigation solutions on protein desorption.
        Int Endod J. 1994; 27: 16-20
        • Spratt D.A.
        • Pratten J.
        • Wilson M.
        • et al.
        An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.
        Int Endod J. 2001; 34: 300-307
        • Sarbinoff J.A.
        • O'Leary T.J.
        • Miller C.H.
        The comparative effectiveness of various agents in detoxifying diseased root surfaces.
        J Periodontol. 1983; 54: 77-80
        • Clegg M.S.
        • Vertucci F.J.
        • Walker C.
        • et al.
        The effect of exposure to irrigant solutions on apical dentin biofilms in vitro.
        J Endod. 2006; 32: 434-437
        • Dunavant T.R.
        • Regan J.D.
        • Glickman G.N.
        • et al.
        Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms.
        J Endod. 2006; 32: 527-531
        • McDonnell G.
        • Russell A.D.
        Antiseptics and disinfectants: activity, action, and resistance.
        Clin Microbiol Rev. 1999; 12: 147-179
        • Moorer W.R.
        • Wesselink P.R.
        Factors promoting the tissue dissolving capability of sodium hypochlorite.
        Int Endod J. 1982; 5: 187-196
        • Baumgartner J.C.
        • Cuenin P.R.
        Efficacy of several concentrations of sodium hypochlorite for root canal irrigation.
        J Endod. 1992; 8: 605-612
        • Gu L.S.
        • Kim J.R.
        • Ling J.
        • et al.
        Review of contemporary irrigant agitation techniques and devices.
        J Endod. 2009; 35: 791-804
        • Huang T.Y.
        • Gulabivala K.
        • Ng Y.L.
        A bio-molecular film ex-vivo model to evaluate the influence of canal dimensions and irrigation variables on the efficacy of irrigation.
        Int Endod J. 2008; 41: 60-71
        • McGill S.
        • Gulabivala K.
        • Mordan N.
        • et al.
        The efficacy of dynamic irrigation using a commercially available system (RinsEndo) determined by removal of a collagen ‘bio-molecular film’ from an ex vivo model.
        Int Endod J. 2008; 41: 602-608
        • Pedrazzi V.
        • Oliveira-Neto J.M.
        • Sepueira P.
        • et al.
        Hand and ultrasonic instrumentation for orthograde root canal treatment of permanent teeth.
        Cochrane Database Syst Rev. 2008; 8 (CD006384)
        • Chopra S.
        • Murray P.E.
        • Namerow K.N.
        A scanning electron microscopic evaluation of the effectiveness of the F-file versus ultrasonic activation of a K-file to remove smear layer.
        J Endod. 2008; 34: 1243-1245
        • Jensen S.A.
        • Walker T.L.
        • Huttler J.W.
        • et al.
        Comparison of the cleaning efficacy of passive sonic activation and passive ultrasonic activation after hand instrumentation in molar root canals.
        J Endod. 1999; 25: 735-738
        • van der Sluis L.W.
        • Versluis M.
        • Wu M.K.
        • et al.
        Passive ultrasonic irrigation of the root canal: a review of the literature.
        Int Endod J. 2007; 40: 415-426
        • Shoji K.
        Alizarin red S staining of calcium compound crystals in synovial fluid.
        Nippon Seikeigeka Gakkai Zasshi. 1993; 67: 201-210
        • Dalton D.C.
        • Ørstavik D.
        • Phillips C.
        • et al.
        Bacterial reduction with nickel titanium instrumentation.
        Int Endod J. 1998; 24: 763-767
        • Ciucchi B.
        • Khettabi M.
        • Holz J.
        The effectiveness of different endodontic irrigation procedures on the removal of the smear layer: a scanning electron microscopic study.
        Int Endod J. 1989; 22: 21-28
        • Zehnder M.
        • Schmidlin P.
        • Sener B.
        • et al.
        Chelation in root canal therapy reconsidered.
        J Endod. 2005; 31: 817-820
        • Foschi F.
        • Nucci C.
        • Montebugnoli L.
        • et al.
        SEM evaluation of canal wall dentine following use of MTwo and ProTaper NiTi rotary instruments.
        Int Endod J. 2004; 37: 832-839
        • Gu L.S.
        • Kim J.R.
        • Ling J.
        • et al.
        Review of contemporary irrigant agitation techniques and devices.
        J Endod. 2009; 35: 791-804
        • Zhang Z.
        • Kim Y.K.
        • Cadenaro M.
        • et al.
        Effects of different exposure times and concentrations of sodium hypochlorite/ethylenediaminetetraacetic acid on the structural integrity of mineralized dentin.
        J Endod. 2010; (accepted for publication)
        • Weller R.N.
        • Brady J.M.
        • Bernier W.E.
        Efficacy of ultrasonic cleaning.
        J Endod. 1980; 6: 740-743
        • Lee S.J.
        • Wu M.K.
        • Wesselink P.R.
        The effectiveness of syringe irrigation and ultrasonics to remove debris from simulated irregularities within prepared root canal walls.
        Int Endod J. 2004; 37: 672-678
        • Townsend C.
        • Maki J.
        An in vitro comparison of new irrigation and agitation techniques to ultrasonic agitation in removing bacteria from a simulated root canal.
        J Endod. 2009; 35: 1040-1043
        • Ahmad M.
        • Pitt Ford T.R.
        • Crum L.A.
        Ultrasonic debridement of root canals: acoustic streaming and its possible role.
        J Endod. 1987; 14: 490-499
        • Ahmad M.
        • Pitt Ford T.R.
        • Crum L.A.
        Ultrasonic debridement of root canals: an insight into the mechanism involved.
        J Endod. 1987; 13: 93-100
        • Ahmad M.
        • Pitt Ford T.R.
        • Crum L.A.
        • et al.
        Ultrasonic debridement of root canals: acoustic cavitation and its relevance.
        J Endod. 1988; 14: 486-493
        • de Gregorio C.
        • Estevez R.
        • Cisneros R.
        • et al.
        Effect of EDTA, sonic, and ultrasonic activation on the penetration of sodium hypochlorite into simulated lateral canals: an in vitro study.
        J Endod. 2009; 35: 891-895
        • Senia E.S.
        • Marshall F.J.
        • Rosen S.
        The solvent action of sodium hypochlorite on pulp tissue of extracted teeth.
        Oral Surg Oral Med Oral Pathol. 1971; 31: 96-103
        • Pesse A.V.
        • Warrier G.R.
        • Dhir V.K.
        An experimental study of the gas entrapment process in closed-end microchannels.
        Int J Heat Mass Transfer. 2005; 48: 5150-5165
        • Tay F.R.
        • Gu L.-S.
        • Schoeffel J.G.
        • et al.
        The effect of vapor lock on root canal debridement using a side-vented needle for positive pressure irrigant delivery.
        J Endod. 2010; (accepted for publication)