Abstract
Introduction
The combination of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) results in
the formation of a precipitate. In a previous study, we demonstrated the formation
of 4-chloroaniline (PCA) in the precipitate by using x-ray photon spectroscopy (XPS)
and time of flight secondary ion mass spectrometry (TOF-SIMS). The TOF-SIMS results
showed a peak at 127 amu, which is characteristic of 4-chloroaniline. However, this
could also be characteristic of other isomers of 4-chloroaniline such as 2-chloroaniline
and 3-chloroaniline.
Aims and Methods
The aim of this study was to further identify the precipitate by using gas chromatography–mass
spectrometry (GC-MS).
Results
The results showed an absence of other aniline derivatives in the precipitate. Only
PCA was found.
Conclusions
Further investigations of the precipitate should address the bioavailability of PCA
leaching out from dentin and its cytotoxicity. Until the precipitate is studied further,
it would appear prudent to minimize its formation by avoiding the use of CHX together
with NaOCl.
Key Words
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References
- The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats.Oral Surg Oral Med Oral Pathol. 1965; 20: 340-349
- Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy.Scand J Dent Res. 1981; 89: 321-328
- Current challenges and concepts in the preparation of root canal systems: a review.J Endod. 2004; 30: 559-567
- Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules.Endod Dent Traumatol. 1990; 6: 142-149
- Combinations of bacterial species in endodontic infections.Int Endod J. 2002; 35: 698-702
- In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution.J Endod. 1999; 25: 167-171
- Antimicrobial activity of 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate separately and combined, as endodontic irrigants.J Endod. 1998; 24: 472-476
- Antibacterial effects of various endodontic irrigants on selected anaerobic bacteria.Endod Dent Traumatol. 1993; 9: 95-100
- A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants.J Endod. 1994; 20: 276-278
- Effectiveness of intracanal irrigants and medications against the yeast Candida albicans.J Endod. 2002; 28: 68-71
- Residual antimicrobial activity after canal irrigation with chlorhexidine.J Endod. 1997; 23: 229-231
- The effect of chlorhexidine gluconate irrigation on the root canal flora of freshly extracted necrotic teeth.Oral Surg Oral Med Oral Pathol. 1982; 53: 518-523
- Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria.J Endod. 1998; 24: 414-416
- Antimicrobial effect of irrigant combinations within dentinal tubules.Int Endod J. 1998; 31: 8-14
- Substantive antimicrobial activity in chlorhexidine-treated human root dentin.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002; 94: 240-245
- Antimicrobial substantivity of chlorhexidine-treated bovine root dentin.J Endod. 2000; 26: 315-317
- Efficacy of chlorhexidine- and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96: 618-624
- Does chlorhexidine have a place in the prophylaxis of dental diseases? review.J Periodontal Res Suppl. 1973; 12: 93-99
- Figueiredo. Dissolution of pulp tissue by aqueous solution of chlorhexidine digluconate and chlorhexidine digluconate gel.Int Endod J. 2004; 37: 38-41
- Root canal irrigants.J Endod. 2006; 32: 389-398
- Influence of irrigants on the coronal microleakage of laterally condensed gutta-percha root fillings.Int Endod J. 2002; 35: 791-795
- Interaction between sodium hypochlorite and chlorhexidine gluconate.J Endod. 2007; 33: 966-969
- Using diazotization to characterize the effect of heat or sodium hypochlorite on 2.0% chlorhexidine.J Endod. 2009; 35: 1296-1299
- Analytical chemistry for technicians.2nd ed. CRC Press, Boca Raton, FL1994
- Microscale and miniscale organic chemistry laboratory experiments.2nd ed. McGraw Hill, New York2004
- Carcinogenicity of p-chloroaniline in rats and mice.Food Chem Toxicol. 1991; 29: 119-124
- Toxicity of 4-chloraniline in early life stages of Zebrafish (Danio rerio): II—cytopathology and regeneration of liver and gills after prolonged exposure to waterborne 4 chloraniline.Arch Environ Contam Toxicol. 1999; 37: 85-102
- Embryonic development assay with Daphnia magna: application to toxicity of aniline derivatives.Chemosphere. 2001; 45: 487-495
- Toxicokinetics and metabolism of aniline and 4-chloroaniline in medaka (Oryzias latipes).Toxicol Appl Pharmacol. 1993; 118: 205-214
Hazardous Substances Data Bank (HSDB). A database of the National Library of Medicines TOXNET System. Available at: http://toxnet.nlm.nih.gov. Accessed February 2007.
Article info
Publication history
Published online: December 14, 2009
Accepted:
October 26,
2009
Received in revised form:
October 21,
2009
Received:
September 25,
2009
Identification
Copyright
© 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
