Abstract
Introduction
The aim of the present study was to investigate the effect of MTAD on the differentiation
of osteoblast-like cells.
Methods
The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1
and periodontal ligament cells at the various concentrations. The bone sialoprotein
(BSP) gene expression was also examined by real-time polymerase chain reaction.
Results
MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication.
The MC3T3-E1 cells treated with H2O2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared
with the control group at the concentration of 50 μg/mlL. No significant difference
in the ALP activity was observed between MTAD and control group. Furthermore, MTAD
and Ca(OH)2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21.
Conclusions
These results suggested that MTAD is a less cytotoxic irrigant and does not affect
differentiation into osteoblasts compared with other intracanal irrigants, such as
H2O2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine.
Key Words
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References
- Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys.Scand J Dent Res. 1981; 89: 475-484
- Bacteria invading periapical cementum.J Endod. 1994; 20: 169-172
- Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM.Endod Dent Traumatol. 1995; 11: 6-9
- A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants.J Endod. 1994; 20: 276-278
- Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy.Scand J Dent Res. 1981; 89: 321-328
- Root canal anatomy of the human permanent teeth.Oral Surg Oral Med Oral Pathol. 1984; 58: 589-599
- Clinical implications of the smear layer in endodontics: a review.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002; 94: 658-666
- Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis.Int Endod J. 1997; 30: 297-306
- Antimicrobial efficacy of various root canal preparation techniques: an in vitro comparative study.J Endod. 2002; 28: 603-605
- Root canal irrigants.J Endod. 2006; 32: 389-398
- Dentinal tubule disinfection using three calcium hydroxide formulations.J Endod. 2005; 31: 50-52
- A new solution for the removal of the smear layer.J Endod. 2003; 29: 170-175
- The antimicrobial effect of MTAD: an in vitro investigation.J Endod. 2003; 29: 400-403
- In vitro antimicrobial efficacy of MTAD and sodium hypochlorite.J Endod. 2003; 29: 450-452
- Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth.J Endod. 2003; 29: 576-579
- The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer.J Endod. 2003; 29: 233-239
- The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline, and citric acid on Enterococcus faecalis.J Endod. 2007; 33: 28-30
- An in vitro comparison of the antimicrobial effects of various endodontic medicaments on Enterococcus faecalis.J Endod. 2007; 33: 567-569
- Evaluation of cytotoxicity of MTAD using the MTT-tetrazolium method.J Endod. 2003; 29: 654-657
- Establishing and characterizing human periodontal ligament fibroblasts immortalized by SV40T-antigen and hTERT gene transfer.Cell Tissue Res. 2006; 324: 117-125
- The biocompatibility of some root canal medicaments and irrigants.Int Endod J. 1981; 14: 115-120
- Biologic assessment of restorative dental materials: interrelationship of biologic and technologic properties.Oper Dent. 1978; 3: 9-13
- Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.J Immunol Methods. 1986; 89: 271-277
- Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites.J Biomed Mater Res. 2005; 72: 127-135
- Responses of MC3T3-E1 cells to three dental resin-based restorative materials.J Biomed Mater Res. 2006; 76: 765-772
- Relationship between alkaline phosphatase levels, osteopontin expression, and mineralization in differentiating MC3T3-E1 osteoblasts.J Cell Biochem. 1998; 68: 269-280
- Phosphophoryn regulates the gene expression and differentiation of NIH3T3, MC3T3-E1, and human mesenchymal stem cells via the integrin/MAPK signaling pathway.J Biol Chem. 2004; 279: 53323-53330
- Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells.J Cell Physiol. 2005; 202: 41-48
- Osteoblast and chondroblast differentiation.Bone. 1995; 17: 77S-83S
- Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis.J Exp Med. 2008; 205: 1145-1153
- Histological evaluation of therapy using a calcium hydroxide dressing for teeth with incompletely formed apices and periapical lesions.J Endod. 1993; 19: 348-352
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© 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
