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Histologic Assessment of Human Pulp Response to Capping with Mineral Trioxide Aggregate and a Novel Endodontic Cement

  • Mohammad Hassan Zarrabi
    Affiliations
    Department of Endodontics, Faculty of Dentistry and Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran, and Iranian Endodontic Research Center, Tehran, Iran
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  • Maryam Javidi
    Affiliations
    Department of Endodontics, Faculty of Dentistry and Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran, and Iranian Endodontic Research Center, Tehran, Iran
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  • Amir Hossein Jafarian
    Correspondence
    Address requests for reprints to Dr Amir Hossein Jafarian, Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Ahmad Abad St. Parastar Av., Mashhad, Khorasan, Iran.
    Affiliations
    Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
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  • Bahareh Joushan
    Affiliations
    Department of Endodontics, Faculty of Dentistry and Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran, and Iranian Endodontic Research Center, Tehran, Iran
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Published:September 16, 2010DOI:https://doi.org/10.1016/j.joen.2010.08.024

      Abstract

      Introduction

      This study was conducted to compare human pulp response to mineral trioxide aggregate (MTA) and a novel endodontic cement (NEC) when used as pulp capping materials after a time period of 2 and 8 weeks.

      Methods

      Thirty-two premolar teeth that were scheduled for extraction for orthodontic reasons were exposed and capped with either MTA or NEC. Half of the specimens underwent extraction and histologic analysis after 2 weeks, and the remaining half were assessed after 8 weeks. Each slide was graded histologically according to the morphology of the dentinal bridge, thickness of the dentinal bridge, presence of odontoblast cells, and inflammation of the pulp.

      Results

      Both MTA and NEC showed significantly better pulp response after 8 weeks compared with 2 weeks, with a thicker and more tubular pattern of the dentinal bridge, less pulp inflammation, and a palisade pattern of odontoblast cells. Although MTA and NEC groups had no significant difference in each measure in both time intervals, NEC induced a thicker dentinal bridge with less pulp inflammation at both 2 weeks and 8 weeks, compared with MTA.

      Conclusions

      MTA and NEC showed similar biocompatibility and favorable response in pulp capping treatment and inducing the formation of the dentinal bridge.

      Key Words

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      References

        • Ingle J.
        • Bakland M.
        • Baumgatner J.
        Endodontics.
        6th ed. BC Decker Inc, Hamilton, Canada2008 (1313)
        • Schröder U.
        Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration, proliferation and differentiation.
        J Dent Res. 1985; 64: 541-548
        • Ford T.R.
        • Torabinejad M.
        • Abedi H.R.
        • Bakland L.K.
        • Kariyawasam S.P.
        Using mineral trioxide aggregate as a pulp-capping material.
        J Am Dent Assoc. 1996; 127: 1491-1494
        • Aeinehchi M.
        • Eslami B.
        • Ghanbariha M.
        • Saffar A.S.
        Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report.
        Int Endod J. 2003; 36: 225-231
        • Junn D.J.
        Quantitative assessment of dentin bridge formation following pulp capping with mineral trioxide aggregate [master’s thesis].
        Loma Linda University, Loma Linda, CA2000
        • Chng H.K.
        • Islam I.
        • Yap A.U.
        • Tong Y.W.
        • Koh E.T.
        Properties of a new root end filling material.
        J Endod. 2005; 31: 665-668
        • Asgary S.
        • Shahabi S.
        • Jafarzadeh T.
        • Amini S.
        • Kheirieh S.
        The properties of a new endodontic material.
        J Endod. 2008; 34: 990-993
        • Asgary S.
        • Eghbal M.J.
        • Parirokh M.
        • Ghoddusi J.
        • Kheirieh S.
        • Brink F.
        Comparison of mineral trioxide aggregate’s composition with Portland cements and a new endodontic cement.
        J Endod. 2009; 35: 243-250
        • Asgary S.
        • Eghbal M.J.
        • Parirokh M.
        Sealing ability of a novel endodontic cement as a root end filling material.
        J Biomed Mater Res. 2008; 87: 706-709
        • Asgary S.
        • Eghbal M.J.
        • Parirokh M.
        • Ghanavati F.
        • Rahimi H.
        A comparative study of histologic response to different pulp capping materials and a novel endodontic cement.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 106: 609-614
        • Asgary S.
        • Parirokh M.
        • Eghbal M.J.
        • Ghoddusi J.
        SEM evaluation of pulp reaction to different pulp capping materials in dog’s teeth.
        Iran Endod J. 2006; 4: 117-122
        • Stanley H.R.
        Pulp capping: conserving the dental pulp—can it be done? is it worth it?.
        Oral Surg Oral Med Oral Pathol. 1989; 68: 628-639
        • Haskell E.W.
        • Stanley H.R.
        • Chellemi J.
        • Stringfellow H.
        Direct pulp capping treatment: a long term follow up.
        J Am Dent Assoc. 1978; 97: 607-612
        • Gomes-Filho J.E.
        • Rodrigues G.
        • Watanabe S.
        • et al.
        Evaluation of the tissue reaction to fast endodontic cement (CER) and Angelus MTA.
        J Endod. 2009; 35: 1377-1380
        • Porter M.L.
        • Bertó A.
        • Primus C.M.
        • Watanabe I.
        Physical and chemical properties of new-generation endodontic materials.
        J Endod. 2010; 36: 524-528
        • Dominguez M.S.
        • Witherspoon D.E.
        • Gutmann J.L.
        • Opperman L.A.
        Histological and scanning electron microscopy assessment of various vital pulp therapy materials.
        J Endod. 2003; 29: 324-333
        • Briso Al
        • Rahal V.
        • Mestrener S.R.
        • Dezan Junior E.
        Biological response of dogs submitted to different capping materials.
        Braz Oral Res. 2006; 20: 219-225
        • Ishizaki N.T.
        • Matsumoto K.
        • Kimura Y.
        • Wang X.
        • Yamashita A.
        Histopathological study of dental pulp tissue capped with enamel matrix derivative.
        J Endod. 2003; 29: 176-179
        • Koliniotou-Koumpia E.
        • Tziafas D.
        Pulpal responses following direct pulp capping of healthy dog teeth with dentin adhesive systems.
        J Dent. 2005; 33: 639-647
      1. Accorinte M de L, Holland R, Reis A, et al. Evaluation of mineral trioxide aggregate and calciumhydroxide cement as pulp capping agents in human teeth. J Endod 2008;34:1–6.

        • Faraco Jr., I.M.
        • Holland R.
        Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement.
        Dent Traumatol. 2001; 17: 163-166
        • Tziafas D.
        • Pantelidou O.
        • Alvanou A.
        • Belibasakis G.
        • Papadimitriou S.
        The dentinogenic effect of mineral trioxide aggregate (MTA) in short term capping experiments.
        Int Endod J. 2002; 35: 245-254
        • Torabinejad M.
        • Hong C.U.
        • McDonald F.
        • Pittford T.R.
        Physical and chemical properties of a new root end filling material.
        J Endod. 1995; 21: 349-353
        • Torabinejad M.
        • Watson T.F.
        • Pittford T.R.
        Sealing ability of a mineral trioxide aggregate when used as a root end filling material.
        J Endod. 1993; 19: 591-595
        • Mitchell P.J.
        • Pittford T.R.
        • Torabinejad M.
        • McDonald F.
        Osteoblast biocompatibility of mineral trioxide aggregate.
        Biomaterials. 1999; 20: 167-173
        • Smith A.J.
        Dentin formation and repair.
        in: Hargreaves K.M. Goodis H.E. Seltzer and Bender’s dental pulp. Quintessence, Chicago2002: 41-62