Abstract
Introduction
It has been suggested that viruses, especially herpesviruses, can play a role in the
pathogenesis of marginal and apical periodontitis. This study aimed to detect herpesviruses
types 1 to 8, namely herpes simplex virus (HSV-1/2), varicella zoster virus (VZV),
Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus-6 (HHV-6),
human herpesvirus-7 (HHV-7), and human herpesvirus-8 (HHV-8) as well as human papillomavirus
(HPV) in acute apical abscesses.
Methods
Twenty-four samples were taken by aspiration of the purulent exudate from acute apical
abscesses. DNA extracted from clinical samples served as a template in single or nested
polymerase chain reaction (PCR) assays for the detection of the target viruses.
Results
Control PCR reactions with β-globin gene primers revealed that all samples but one
had detectable human DNA. Of the 23 abscess samples positive for the β-globin gene,
14 (61%) were positive for at least one of the target human viruses. Thirteen (56.5%)
cases had herpesvirus: HHV-8 occurred in 11 (48%), VZV and HHV-6B in two (9%), and
HHV-7 and HSV-1/2 in one (4%). EBV and HCMV were not present in any of the examined
samples. HPV was detected in three (13%) abscess samples. Viral coinfection was found
in five cases, with one case harboring three of the targeted viruses.
Conclusion
A large number of abscess samples were positive for at least one target virus. Unexpectedly,
HHV-8 was for the first time detected and in a high prevalence. Papillomavirus and
other herpesviruses were also found for the first time in endodontic abscesses. Although
these findings suggest an association, the specific role of viruses in the pathogenesis
of acute apical abscesses awaits further clarification.
Key Words
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References
- The microbiology of the acute dental abscess.J Med Microbiol. 2009; 58: 155-162
- Bacterial pathogenesis and mediators in apical periodontitis.Braz Dent J. 2007; 18: 267-280
- Herpesviruses in periapical pathosis: an etiopathogenic relationship?.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96: 327-331
Slots J. Herpesviruses in periodontal diseases. Periodontol 2000 2005;38:33–62.
- Human cytomegalovirus and Epstein-Barr virus type 1 in periodontal abscesses.Oral Microbiol Immunol. 2004; 19: 83-87
- Cytomegalovirus and Epstein-Barr virus are associated with symptomatic periapical pathosis.Oral Microbiol Immunol. 2003; 18: 327-328
- Cytomegalovirus and Epstein-Barr virus DNA transcription in endodontic symptomatic lesions.Oral Microbiol Immunol. 2003; 18: 104-108
- Herpesviral-bacterial coinfection in periapical pathosis.J Endod. 2004; 30: 69-72
- Human cytomegalovirus is present in odontogenic cysts.Oral Microbiol Immunol. 2007; 22: 347-351
- Herpesviruses in asymptomatic apical periodontitis lesions: an immunohistochemical approach.Oral Microbiol Immunol. 2007; 22: 320-325
- Herpesviruses in endodontic pathoses: association of Epstein-Barr virus with irreversible pulpitis and apical periodontitis.J Endod. 2009; 35: 23-29
- Herpesviruses in abscesses and cellulitis of endodontic origin.J Endod. 2009; 35: 182-188
Slots J. Oral viral infections of adults. Periodontol 2000 2009;49:60–86.
- Detection of human viruses in periodontal pockets using polymerase chain reaction.Oral Microbiol Immunol. 1996; 11: 289-293
- Marginal periodontium as a potential reservoir of human papillomavirus in oral mucosa.J Periodontol. 2005; 76: 358-363
- Search for evidence of three viral agents in radicular (periapical) cysts with immunohistochemistry.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995; 80: 87-91
- Cervical cytology biobanking: quality of DNA from archival cervical Pap-stained smears.J Clin Pathol. 2008; 61: 637-641
- Development of a multiplex nested consensus PCR for detection and identification of major human herpesviruses in CNS infections.J Clin Virol. 2005; 32: 318-324
- Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR.J Clin Microbiol. 2003; 41: 1565-1568
- Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay.J Clin Microbiol. 2001; 39: 4426-4432
- Lymphotropic herpesviruses in allogeneic bone marrow transplantation.Blood. 1996; 88: 3615-3620
- Association between human herpesvirus type 6 and type 7, and cytomegalovirus disease in heart transplant recipients.Transplant Proc. 2002; 34: 75-76
- Detection of human herpesvirus 8 DNA in pemphigus and chronic blistering skin diseases.J Korean Med Sci. 2000; 15: 442-448
- BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucleic Acids Symp Ser. 1999; 41: 95-98
- Basic local alignment search tool.J Mol Biol. 1990; 215: 403-410
- Molecular pathways in virus-induced cytokine production.Microbiol Mol Biol Rev. 2001; 65: 131-150
- The role of viruses in oral disease.J Oral Microbiol. 2010; 2: 2127
- Herpesviruses in human periodontal disease.J Periodontal Res. 2000; 35: 3-16
- Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s sarcoma.Science. 1994; 266: 1865-1869
- Spectrum of Kaposi’s sarcoma-associated herpesvirus, or human herpesvirus 8, diseases.Clin Microbiol Rev. 2002; 15: 439-464
- Mother-to-child transmission of human herpesvirus-8 in South Africa.J Infect Dis. 2004; 190: 1068-1075
- Mucosal shedding of human herpesvirus 8 in men.N Engl J Med. 2000; 343: 1369-1377
- Herpesviruses 6, 7 and 8 in HIV- and non-HIV-associated periodontitis.J Periodontal Res. 2000; 35: 278-284
- Frequent detection of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) DNA in saliva of human immunodeficiency virus-infected men: clinical and immunologic correlates.J Infect Dis. 1997; 176: 94-102
- Herpesviruses in HIV-periodontitis.J Clin Periodontol. 2001; 28: 96-102
- High prevalence of multiple human herpesviruses in saliva from human immunodeficiency virus-infected persons in the era of highly active antiretroviral therapy.J Clin Microbiol. 2006; 44: 2409-2415
- Review of the distribution of Kaposi’s sarcoma-associated herpesvirus (KSHV) in Africa in relation to the incidence of Kaposi’s sarcoma.Br J Cancer. 2003; 88: 1-3
- Seroprevalence of HHV-8, CMV, and EBV among the general population in Ghana, West Africa.BMC Infect Dis. 2008; 8: 111
- Human herpesvirus 8 in Brazilian Amerindians: a hyperendemic population with a new subtype.J Infect Dis. 2000; 181: 1562-1568
- Increasing seroprevalence of human herpesvirus 8 (HHV-8) with age confirms HHV-8 endemicity in Amazon Amerindians from Brazil.J Gen Virol. 2005; 86: 2433-2437
- Kaposi’s sarcoma herpesvirus as a new paradigm for virus-induced oncogenesis.Curr Opin Oncol. 1998; 10: 405-412
- Seroprevalence of human herpesvirus 8 and its association with Kaposi sarcoma in Brazil.Sex Transm Dis. 2000; 27: 468-472
- Seroprevalence of human herpesvirus-8 in blood donors from different geographical regions of Argentina, Brazil, and Chile.J Med Virol. 2004; 72: 661-667
- Prevalence of, and risk factors for Kaposi’s sarcoma-associated herpesvirus infection among blood donors in Brazil: a multi-center serosurvey.J Med Virol. 2008; 80: 1202-1210
- Herpesviruses in periodontal pocket and gingival tissue specimens.Oral Microbiol Immunol. 2000; 15: 15-18
- Is herpes simplex virus associated with pulp/periapical inflammation?.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001; 91: 359-361
- Human cytomegalovirus and Epstein-Barr virus in apical and marginal periodontitis: a role in pathology?.J Med Virol. 2008; 80: 1007-1011
Slots J. Herpesviral-bacterial interactions in periodontal diseases. Periodontol 2000 2010;52:117–140.
- Patient with severe periodontitis and subgingival Epstein-Barr virus treated with antiviral therapy.J Clin Virol. 2008; 42: 176-178
Article info
Publication history
Published online: November 15, 2010
Footnotes
Supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazilian Governmental Institutions.
Identification
Copyright
© 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
