Abstract
Introduction
This study proposed to investigate aspects of cell proliferation and death in the
epithelium of radicular (RCs) and dentigerous (DCs) cysts.
Methods
Serial sections of 17 RCs and 9 DCs were prepared for immunohistochemical detection
of caspase-3, Bcl-2, and Ki-67 antigens.
Results
Caspase-3 was detected mainly in the suprabasal and superficial epithelial cells of
RCs and DCs, whereas Ki-67 was detected predominantly in the basal layer. Both markers
had significant expression in hyperplastic epithelium related to an intense inflammation
in the capsule. Immunoreactivity for Bcl-2 was restricted to the basal layer and was
significantly higher in atrophic epithelium of DCs than that of RCs.
Conclusions
These results suggest that epithelial proliferation is balanced by apoptosis and that
the presence of inflammation inhibits the Bcl-2 expression. DCs and RCs have different
formation mechanisms but have similar biological behavior in the presence of intense
inflammatory infiltrate.
Key Words
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© 2011 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
