Abstract
Introduction
Calcium silicate–based materials (CSMs) are used in various endodontic procedures.
The present study examined whether prolonged contact of mineralized dentin with recently
commercialized versions of these materials adversely affects dentin collagen matrix
integrity.
Methods
Dentin slabs prepared from extracted human third molars (7 × 3 × 0.3 mm) were divided
into 3 groups on the basis of the material to which dentin was exposed (MTA Plus,
Biodentine, untreated control dentin slabs) and the time period of exposure (24 hours,
1, 2, and 3 months; n = 6). Hydroxyproline assay was performed on each group’s supernatant
to quantify the collagen extraction amounts of each group per time period. Data were
analyzed with two-factor repeated-measures analysis of variance and Holm-Sidak pair-wise
comparisons (α = 0.05) to determine the effects of material and aging time on collagen
extraction. Dentin slabs from the 3 months of aging group were demineralized for transmission
electron microscopy examination of collagen matrix ultrastructural changes.
Results
Material (P = .002), aging time (P < .001), and their interactions (P = .007) significantly affected the amount of hydroxyproline (pg/mg of mineralized
dentin) extracted from mineralized dentin and were significantly correlated by power
regression models. Collagen degradation was identified from the surface of dentin
slabs that were in direct contact with CSMs.
Conclusions
Prolonged contact of mineralized dentin with CSMs has an adverse effect on the integrity
of the dentin collagen matrix. However, the amount of collagen extracted was limited
to the contact surface. Clinicians can continue to apply CSMs in endodontic procedures;
however, caution is advised when these materials are applied to thin dentinal walls.
Key Words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of EndodonticsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- A prospective clinical study of periradicular surgery using mineral trioxide aggregate as a root-end filling.J Endod. 2008; 34: 660-665
- Mineral trioxide aggregate: a comprehensive literature review—part III: clinical applications, drawbacks, and mechanism of action.J Endod. 2010; 36: 400-413
- Mineral trioxide aggregate: a comprehensive literature review—part I: chemical, physical and antibacterial properties.J Endod. 2010; 36: 16-27
- Mineral trioxide aggregate: a comprehensive literature review—part II: leakage and biocompatibility investigations.J Endod. 2010; 36: 190-202
- Periapical bone regeneration after endodontic microsurgery with three different root-end filling materials: amalgam, SuperEBA, and mineral trioxide aggregate.J Endod. 2010; 36: 1323-1325
- Sealing ability of a mineral trioxide aggregate when used as a root end filling material.J Endod. 1993; 19: 591-595
- Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations.J Endod. 1993; 19: 541-544
- Mineral trioxide aggregate or calcium hydroxide direct pulp capping: an analysis of the clinical treatment outcome.J Endod. 2010; 36: 806-813
- Clinical applications of mineral trioxide aggregate.J Endod. 1999; 25: 197-205
- Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial.Int Endod J. 2008; 41: 128-150
- Reparative dentinogenesis induced by mineral trioxide aggregate: a review from the biological and physicochemical points of view.Int J Dent. 2009; 2009: 464280
- Mineral trioxide aggregate obturation: a review and case series.J Endod. 2009; 35: 777-790
- Apexification of immature teeth with calcium hydroxide or mineral trioxide aggregate: systematic review and meta-analysis.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011; 112: e36-e42
- The use of mineral trioxide aggregate in one-visit apexification treatment: a prospective study.Int Endod J. 2007; 40: 186-197
- Management of trauma-induced inflammatory root resorption using mineral trioxide aggregate obturation: two-year follow up.Dent Traumatol. 2010; 26: 501-504
- Dissolution of bio-active dentine matrix components by mineral trioxide aggregate.J Dent. 2007; 35: 636-642
- Characterization of hydration products of mineral trioxide aggregate.Int Endod J. 2008; 41: 408-417
- The effect of three commonly used endodontic materials on the strength and hardness of root dentin.J Endod. 2002; 28: 828-830
- Effect of exposing dentine to sodium hypochlorite and calcium hydroxide on its flexural strength and elastic modulus.Int Endod J. 2001; 34: 113-119
- Long-term calcium hydroxide as a root canal dressing may increase risk of root fracture.Dent Traumatol. 2002; 18: 134-137
- Fracture resistance of human root dentin exposed to intracanal calcium hydroxide.J Endod. 2005; 31: 895-897
- Effect of orientation on the in vitro fracture toughness of dentin: the role of toughening mechanisms.Biomaterials. 2003; 24: 3955-3968
- Aging and the reduction in fracture toughness of human dentin.J Mech Behav Biomed Mater. 2009; 2: 550-559
- Considerations in the selection of a root-end filling material.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999; 87: 398-404
- Physical and chemical properties of a new root-end filling material.J Endod. 1995; 21: 349-353
- Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements.Dent Mater. 2005; 21: 731-738
- Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine.Int Endod J. 2011; 44: 1081-1087
- Induction of specific cell responses to a Ca(3)SiO(5)-based posterior restorative material.Dent Mater. 2008; 24: 1486-1494
- Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate.Acta Biomater. 2012; 8: 836-842
- Host-derived loss of dentin stiffness associated with solubilization of collagen.J Biomed Mater Res Part B Appl Biomater. 2009; 90B: 373-380
- A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues.Anal Biochem. 1981; 15: 70-75
- The inhibitory effect of polyvinylphosphonic acid on functional MMP activities in human demineralized dentin.Acta Biomater. 2010; 6: 4136-4142
- The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs.J Dent Res. 2011; 90: 535-540
- Dentin collagen: chemical structure and role in mineralization.in: Linde A. Dentin and dentinogenesis. vol II. CRC Press, Boca Raton, FL1984: 40
- The survival of organic matter in bone: a review.Archaeometry. 2002; 44: 383-394
Article info
Publication history
Published online: February 02, 2012
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
