Abstract
Introduction
It is suggested that dental pulp stem cells are involved in tooth regeneration and
play an important role in maintaining pulp homeostasis. Previously, normal dental
pulps were more widely used for experimental models than carious dental pulps. The
aim of this study was to isolate and culture the dental pulp stem cells from carious
and normal teeth and to evaluate stem cell parameters.
Methods
Pulp tissues were obtained and dissociated from normal and carious teeth. Single-cell
suspensions were seeded into 6-well plates and purified by collecting multiple colonies.
Normal dental pulp stem cells (DPSCs) and carious dental pulp stem cells (CDPSCs)
were compared for morphologic appearance and for their capacity to differentiate into
3 lineages. Colony-forming and MTT assays, cell cycle analysis, gene expression, and
alkaline phosphatase activity were also evaluated.
Results
Stem cells were cultured successfully from normal and carious dental pulps. CDPSCs
displayed increased proliferation ability compared with DPSCs. CDPSCs also showed
enhanced ALP activity, mineralization ability, and expression of osteogenesis/dentinogenesis-related
genes. All cultures differentiated into 3 cell types.
Conclusions
Our data suggest that caries as a local microenvironment should be taken into account
when DPSCs are intended to be used for investigations and application. Furthermore,
the mechanism of the underlying changes in cell properties requires further study.
Key Words
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Article info
Publication history
Published online: March 26, 2012
Footnotes
Supported by a grant-in-aid for College intelligence funds from Guangdo-ng Province (no. C1010047) and Events of Science and Technology Program of Guang Dong Province (no. 2010B060900053).
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
