Macrophages are highly activated by endodontic contents. This study investigated the correlation between different clinical signs/symptoms and radiographic features according to the levels of interleukin (IL)-1β, tumor necrosis factor α (TNF-α), IL-6, IL-10, prostaglandin E2 (PGE2), and their networks produced by endodontic content–stimulated macrophages collected from primary endodontic infection with apical periodontitis (PEIAP).
Samples were taken from 21 root canals with PEIAP by using paper points. The presence of exudate (EX), pain on palpation (POP), tenderness to percussion (TTP), and the size of the radiographic lesion (SRL) were recorded. Polymerase chain reaction (16S rDNA) was used for bacterial detection and limulus amebocyte lysate (LAL) assay for endotoxin measurement. Raw 264.7 macrophages were stimulated with bacterial contents during 24 hs. The amounts of IL-1β, TNF-α, IL-6, IL-10 and PGE2 were measured by enzyme-linked immunosorbent assay. Log-based data were correlated by multiple logistic regression (P < .05).
Bacteria and endotoxin were detected in 100% of the samples. IL-6 and TNF-α were positively correlated with SRL and EX, respectively (P < .05). Clinical signs/symptoms and radiographic findings were set as dependent variables for EX-positive correlations between PGE2, IL-1β, and TNF-α (P < .05), whereas IL-6 and PGE2 were positively correlated to each other in POP but negatively correlated in SRL (P < .05). When POP and TTP-POP were set as dependent variables, different cytokine networks were found.
Our findings suggest different roles for each cytokine in the development of apical periodontitis, whose effects of overlapping networks depend on the signs/symptoms and radiographic features found in endodontic infection.
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- The effects of surgical exposures of dental pulps in germ-free and conventional rats.Oral Surg Oral Med Oral Pathol. 1965; 20: 340-349
- Pathogenic mechanisms in pulpal disease.J Endod. 1990; 16: 98-101
- Pathogenesis of apical periodontitis and the causes of endodontic failures.Crit Rev Oral Med. 2004; 15: 348-381
- Cytokines in periodontal disease: where to from here?.Acta Odontol Scand. 2001; 59: 167-173
- Tumor necrosis factor in 1998.Cytokine Growth Factor Rev. 1998; 9: 297-302
- TNF-alpha and pathologic bone resorption.Keio J Med. 2005; 54: 127-131
- Effect of interleukin-6 deficiency on the formation of periapical lesions after pulp exposure in mice.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001; 92: 83-88
- IL-6 is produced by osteoblasts and induces bone resorption.J Immunol. 1990; 145: 3297-3303
- Prostaglandin E2 inhibits interleukin-6 release but not its transcription in human gingival fibroblasts stimulated with interleukin-1 beta or tumor necrosis factor-alpha.J Periodontol. 1994; 65: 1122-1127
- Diversity in the contribution of interleukin-10 to T-cell-mediated immune regulation.Immunol Rev. 2008; 226: 219-233
- IL-10 inhibits cytokine production by activated macrophages.J Immunol. 1991; 147: 3815-3822
- Interleukin-10 reduces inflammation, endothelial dysfunction, and blood pressure in hypertensive pregnant rats.Am J Physiol Regul Integr Comp Physiol. 2010; 298: 713-719
- Macrophages in periapical lesions.Endod Dent Traumatol. 2000; 16: 1-8
- Influence of combinations of oral bacteria on periapical tissues of monkeys.Scand J Dent Res. 1982; 90: 200-206
- Bacterial endotoxins.Sci Am. 1992; 267: 54-61
- Gamma interferon modulation of prostaglandin E2 release from monocytes stimulated with lipopolysaccharides from Bacteroides intermedius, Bacteroides gingivalis, and Salmonella typhimurium.Oral Microbiol Immunol. 1988; 3: 138-143
- The role of lipopolysaccharide in infectious bone resorption of periapical lesion.J Oral Pathol Med. 2004; 33: 162-169
- Interleukin 1β in symptomatic and asymptomatic human perirradicular lesions.J Endod. 1994; 20: 225-227
- Detection of interleukin-1 beta in human periapical lesions.Oral Surg Oral Med Oral Pathol. 1992; 73: 334-336
- Tumor necrosis factor identified in periapical tissue exudates of teeth with apical periodontitis.J Endod. 1991; 17: 12-14
- Immunoreactivity for interleukin 1-beta and tumor necrosis factor-alpha and ultrastructural features of monocytes/macrophages in periapical granulomas.J Endod. 1991; 17: 483-487
- Quantitative analysis of immunocompetent cells in human periapical lesions: correlations with clinical findings of the involved teeth.J Endod. 1992; 18: 497-500
- Interleukin-1beta and tumour necrosis factor-alpha levels in periapical exudates.Int Endod J. 2002; 35: 181-185
- Immunolocalization of bone-resorptive cytokines in rat pulp and periapical lesions following surgical pulp exposure.Oral Microbiol Immunol. 1995; 10: 213-219
- A radioimmunoassay determination of the concentrations of prostaglandins E2 and F2 alpha in painful and asymptomatic human dental pulps.J Endod. 1985; 11: 330-335
- The change of periapical exudate prostaglandin E2 levels during root canal treatment.J Endod. 1997; 23: 755-758
- The concentration of prostaglandin E2 in human periradicular lesions.J Endod. 1991; 17: 97-100
- Detection of interleukin-6 in human dental pulp and periapical lesions.Endod Dent Traumatol. 1999; 15: 26-27
- Quantification of endotoxins and cultivable bacteria in root canal infection before and after chemomechanical preparation with 2.5% sodium hypochlorite.J Endod. 2008; 34: 268-272
- Antigenic activity of bacterial endodontic contents from primary root canal infection with periapical lesions against macrophage in the release of interleukin-1beta and tumor necrosis factor alpha.J Endod. 2010; 36: 1766-1769
- Quantification of endotoxins in necrotic root canals from symptomatic and asymptomatic teeth.J Med Microbiol. 2005; 54: 777-783
- The synergistic effect of peptidoglycan and lipopolysaccaride on osteoclast formation.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96: 738-743
- Periapical inflammatory responses and their modulation.Crit Rev Oral Biol Med. 1998; 9: 498-521
- Actinobacillus actinomycetemcomitans lipopolysaccharide induces interleukin-6 expression through multiple mitogen-activated protein kinase pathways in periodontal ligament fibroblasts.Oral Microbiol Immunol. 2006; 21: 392-398
- Cytokine regulation of localized inflammation; induction of activated B cells and IL-6-mediated polyclonal IgG an IgA synthesis in inflamed human gingiva.J Immunol. 1991; 146: 1812-1821
- B cell differentiation factor in synovial fluid of patients with rheumatoid arthritis.Immunol Rev. 1984; 78: 7-23
- Effect of periodontal treatment on metabolic control, systemic inflammation and cytokines in patients with type 2 diabetes.J Clin Periodontol. 2010; 37: 53-58
- Tissue levels of bone resorptive cytokines in periodontal diseases.J Periodontol. 1991; 62: 504-509
- Distribution of Porphyromonas gingivalis fimA genotypes in chronic apical periodontitis associated with symptoms.J Endod. 2010; 36: 1790-1795
- Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings.J Endod. 2010; 36: 1277-1288
- Genetic predisposition to persistent apical periodontitis.J Endod. 2011; 37: 455-459
- Cytokine analysis in lesions refractory to endodontic treatment.J Endod. 2011; 37: 1659-1662
- Biologic markers for odontogenic periradicular periodontitis.J Endod. 2010; 36: 1307-1310
- Relationship between Fcgamma receptor and interleukin-1 gene polymorphisms and post-treatment apical periodontitis.J Endod. 2009; 35: 1186-1192
Published online: April 20, 2012
Supported by the Brazilian agencies FAPESP (08/57551-0; 08/56425; 10/51113-1; 10/17877-4 10/19136-1; 11/50510-0) and CNPq (302575/2009-0; 150557/2011-6).
© 2012 Published by Elsevier Inc.