Abstract
Introduction
In previous studies, we found expression of stromal cell–derived factor-1α (SDF-1α)/CXC
chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α–CXCR4 axis might
play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4+ DPCs) toward the damaged sites. However, the specific function of CXCR4+ DPCs in the injured dental pulp was still unknown. The purpose of this study was
to isolate CXCR4+ DPCs from dental pulp cells in vitro to pave the way for further study of their characteristics.
Methods
CXCR4+ DPCs were isolated with magnetic-activated cell sorting (MACS). Freshly isolated
CXCR4+ DPCs were identified by immunohistochemistry with light microscopy or confocal microscopy.
Then the phenotypes CXCR4, stromal cell surface marker-1 (STRO-1), CD146, and CD34
in 3 groups (ie, CXCR4+ DPCs, CXCR4− DPCs, or non-sorted DPCs) were analyzed by flow cytometry after they were cultured
and expanded in vitro.
Results
The results indicated the isolated subpopulation of DPCs was enriched with CXCR4+ DPCs, and the positive rates of STRO-1 and CD146 in CXCR4+ DPCs group were higher than CXCR4− DPCs or non-sorted DPCs groups (P < .05). There was no expression of CD34 in each group.
Conclusions
We can isolate CXCR4+ DPCs from DPCs with MACS and identify them by immunohistochemistry and flow cytometry.
Key Words
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Article info
Publication history
Published online: April 09, 2012
Footnotes
Supported by grants from the Science and Technology Commission of Shanghai Municipality (grant no. 08DZ2271100, 08JC1414500), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (grant no. 2008-0890-09), Innovation Program of Shanghai Municipal Education Commission (grant no. 09ZZ116), and the selection and cultivation for outstanding young teachers in scientific special fund of Shanghai university (grant no. JDY09051).
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
