Abstract
Introduction
Anandamide (N-arachidonoylethanolamine [AEA]) is one of the main endocannabinoids. Endocannabinoids
are implicated in various physiological and pathologic functions, inducing not only
nociception but also regeneration and inflammation. The role of the endocannabinoid
system in peripheral organs was recently described. The aim of this study was to investigate
the effect of AEA on matrix metalloproteinase (MMP)-2 induction in human dental pulp
cells (HPC).
Methods
We examined AEA-induced MMP-2 production and the expression of AEA receptors (cannabinoid
[CB] receptor-1, CB2, and transient receptor potential vanilloid-1 [TRPV1]) in HPC
by Western blot. MMP-2 concentrations in supernatants were determined by enzyme-linked
immunosorbent assay. We then investigated the role of the AEA receptors and mitogen-activated
protein kinase in AEA-induced MMP-2 production in HPC.
Results
AEA significantly induced MMP-2 production in HPC. HPC expressed all 3 types of AEA
receptor (CB1, CB2, and TRPV1). AEA-induced MMP-2 production was blocked by CB1 or
TRPV1 antagonists and by small interfering RNA for CB1 or TRPV1. Furthermore, c-Jun
N-terminal kinase inhibitor also reduced MMP-2 production.
Conclusions
We demonstrated for the first time that AEA induced MMP-2 production via CB1 and TRPV1
in HPC.
Key Words
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Article info
Publication history
Published online: April 13, 2012
Footnotes
Supported by Grants-in-Aid for Scientific Research (#22592125 and #22592124) from the Ministry of Education, Science, and Culture of Japan.
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
