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Effect of Ultrasonic Activation on pH and Calcium Released by Calcium Hydroxide Pastes in Simulated External Root Resorption

Published:April 26, 2012DOI:https://doi.org/10.1016/j.joen.2012.03.005

      Abstract

      Introduction

      The purpose of this study was to analyze the influence of ultrasonic activation of calcium hydroxide (CH) pastes on pH and calcium release in simulated external root resorptions.

      Methods

      Forty-six bovine incisors had their canals cleaned and instrumented, and defects were created in the external middle third of the roots, which were then used for the study. The teeth were externally made impermeable, except for the defected area, and divided into the following 4 groups containing 10 samples each according to the CH paste and the use or not of the ultrasonic activation: group 1: propylene glycol without ultrasonic activation, group 2: distilled water without ultrasonic activation, group 3: propylene glycol with ultrasonic activation, and group 4: distilled water with ultrasonic activation. After filling the canals with the paste, the teeth were restored and individually immersed into flasks with ultrapure water. The samples were placed into other flasks after 7, 15, and 30 days so that the water pH level could be measured by means of a pH meter. Calcium release was measured by means of an atomic absorption spectrophotometer. Six teeth were used as controls. The results were statistically compared using the Kruskal-Wallis and Mann-Whitney U tests (P < .05).

      Results

      For all periods analyzed, the pH level was found to be higher when the CH paste was activated with ultrasound. Calcium release was significantly greater (P < .05) using ultrasonic activation after 7 and 30 days.

      Conclusions

      The ultrasonic activation of CH pastes favored a higher pH level and calcium release in simulated external root resorptions.

      Key Words

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      References

        • George G.K.
        • Rajkumar K.
        • Sanjeev K.
        • Mahalaxmi S.
        Calcium ion diffusion levels from MTA and apexcal in simulated external root resorption at middle third of the root.
        Dent Traumatol. 2009; 25: 480-483
      1. Pacios MG, de La Casa ML de, Bulacio M de los A, Lopez ME. Influence of different vehicles on the pH of calcium hydroxide pastes. J Oral Sci 2004;46:107–11.

        • Yücel A.C.
        • Aksoy A.
        • Ertas E.
        • Güvenc D.
        The pH changes of calcium hydroxide mixed with six different vehicles.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007; 103: 712-717
        • Heward S.
        • Sedgley C.M.
        Effects of intracanal mineral trioxide aggregate and calcium hydroxide during four weeks on pH changes in simulated root surface resorption defects: an in vitro study using matched Pairs of Human Teeth.
        J Endod. 2011; 37: 40-44
        • Tronstad L.
        • Andreasen J.O.
        • Hasselgren G.
        • Kristerson L.
        • Riis I.
        pH changes in dental tissues after root canal filling with calcium hydroxide.
        J Endod. 1981; 7: 17-21
        • Wang J.D.
        • Hume W.R.
        Diffusion of hydrogen ion and hydroxyl ion from various sources of through dentine.
        Int Endod J. 1988; 21: 17-26
        • Nerwich A.
        • Figdor D.
        • Messer H.H.
        pH changes in root dentin over a 4 week period following root canal dressing with calcium hydroxide.
        J Endod. 1993; 19: 302-306
        • Tsesis I.
        • Lin S.
        • Weiss E.I.
        • Fuss Z.
        Dentinal pH changes following electrophoretically activated clacium hydroxide ions in the root canal space of bovine teeth.
        Dent Traumatol. 2005; 21: 146-149
        • Çalt S.
        • Serper A.
        • Özçelik B.
        • Dalat M.D.
        pH changes and calcium ion diffusion from calcium hydroxide dressing materials through root dentin.
        J Endod. 1999; 25: 329-331
        • Javelet J.
        • Torabinejad M.
        • Bakland L.K.
        Comparison of two pH levels for the induction of apical barriers in immature teeth of monkeys.
        J Endod. 1985; 11: 375-378
        • Plotinno G.
        • Pameijer C.H.
        • Grande N.M.
        • Somma F.
        Ultrasonics in endodontics: a review of the literature.
        J Endod. 2007; 33: 81-95
        • Wiseman A.
        • Cox T.C.
        • Paranjpe A.
        • Flake N.M.
        • Cohenca N.
        • Johnson J.D.
        Efficacy of sonic and ultrasonic activation for removal of calcium hydroxide from mesial canals of mandibular molars: a microtomographic study.
        J Endod. 2011; 37: 235-238
        • Camargo C.H.R.
        • Bernardineli N.
        • Valera M.C.
        • et al.
        Vehicle influence on calcium hydroxide pastes diffusion in human and bovine teeth.
        Dent Traumatol. 2006; 22: 302-306
        • Zmener O.
        • Pameijer C.H.
        • Banegas G.
        An in vitro study of the pH of three calcium hydroxide dressing materials.
        Dent Traumatol. 2007; 23: 21-25
        • Fuss Z.
        • Rafaeloff R.
        • Tagger M.
        • Szajkis S.
        Intracanal pH changes of calcium hydroxide pastes exposed to carbon dioxide in vitro.
        J Endod. 1996; 22: 362-364
        • Saif S.
        • Carey C.M.
        • Tordik P.A.
        • McClanahan S.B.
        Effects of irrigants and cementum injury on diffusion of hydroxyl ion through the dentinal tubules.
        J Endod. 2008; 34: 50-52
        • Simon S.T.
        • Bhat K.S.
        • Francis R.
        Effect of four vehicles on the pH of calcium hydroxide and the release of calcium ion.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995; 80: 459-464
        • Duarte M.A.H.
        • Midena R.Z.
        • Zeferino M.A.
        • et al.
        Evaluation of pH and calcium ion release of calcium hydroxide pastes containing different substances.
        J Endod. 2009; 35: 1274-1277
        • Estrela C.
        • Pesce H.F.
        Chemical analysis of the liberation of calcium and hydroxyl ions from calcium hydroxide pastes in connective tissue in the dog—part I.
        Braz Dent J. 1996; 7: 41-46
        • Haenni S.
        • Schmidilin P.R.
        • Mueller B.
        • Sener B.
        • Zehnder M.
        Chemical and antimicrobial properties of calcium hydroxide mixed with irrigant solutions.
        Int Endod J. 2003; 36: 100-105
        • Evans M.
        • Davies J.K.
        • Sundqvist G.
        • Figdor D.
        Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide.
        Int Endod J. 2002; 35: 221-228
        • Appelbe O.K.
        • Sedgley C.M.
        Effects of prolonged exposure to alkaline pH on Enterococcus faecalis survival and specific gene transcripts.
        Oral Microbiol Immunol. 2007; 22: 169-174
        • Cruz E.V.
        • Kota K.
        • Huque J.
        • Iwaku M.
        • Hoshino E.
        Penetration of propylene glycol into dentine.
        Int Endod J. 2002; 35: 330-336
        • Duarte M.A.
        • Martins C.S.
        • Demarchi A.C.O.C.
        • Godoy L.F.
        • Kuga M.C.
        • Yamashita J.C.
        Calcium and hydroxide release from different pulp-capping material.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007; 104: e66-e69
        • Narita H.
        • Itoh S.
        • Imazato S.
        • Yoshitake F.
        • Ebisu S.
        An explanation of the mineralization mechanism in osteoblasts induced by calcium hydroxide.
        Acta Biomater. 2010; 6: 586-590