Abstract
Introduction
The equilibrium of inhibitors and inducers plays an important role in the mineralization
of dentin during dentinogenesis. High-temperature requirement protein A1 (HtrA1) is
a novel bone mineralization inhibitor involved in physiological and pathological bone
formation. However, the expression of HtrA1 in tooth mineralization is unknown. The
purpose of the present study was to investigate the localization and quantity of HtrA1
and its possible related molecules during induced reparative dentin formation.
Methods
Rats were randomly sacrificed after direct pulp capping on days 0, 7, 14, and 21.
Maxillary segments were obtained and routinely prepared for histological analysis,
immunohistochemistry, quantum dots–based double immunofluorescence, and CRi’s Nuance
imaging system (CRI, Woburn, MA)–based quantitative determination.
Results
The square measure values of reparative dentin significantly increased on day 7 and
continued to increase until day 21. HtrA1, matrix Gla protein (MGP), nestin, and bone
sialoprotein were positively stained and colocalized in the odontoblasts and/or odontoblast-like
cells zone and reparative dentin during induced reparative dentin formation. The expressions
of HtrA1 and MGP were significantly enhanced after direct pulp capping on day 7 and
did not significantly change between days 7, 14, and day 21. Both expressions of HtrA1
and MGP were positively correlated with the square measure values of reparative dentin;
however, no correlation was found between the expressions of HtrA1 and MGP.
Conclusions
HtrA1 could be observed and might possibly be involved in the process of reparative
dentin formation associated with MGP.
Key Words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of EndodonticsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Dentinogenesis.Crit Rev Oral Biol Med. 1993; 4: 679-728
- Dental lesions in tumoral calcinosis.J Oral Pathol Med. 1991; 20: 222-227
- The HtrA family of proteases: implications for protein composition and cell fate.Mol Cell. 2002; 10: 443-455
- Primary structure of a putative serine protease specific for IGF-binding proteins.FEBS Lett. 1996; 398: 187-192
- The HtrA1 serine protease is down-regulated during human melanoma progression and represses growth of metastatic melanoma cells.Oncogene. 2002; 21: 6684-6688
- LOC387715/HTRA1 gene polymorphisms and susceptibility to age-related macular degeneration: a HuGE review and meta-analysis.Mol Vis. 2010; 16: 1958-1981
- HtrA1-dependent proteolysis of TGF-beta controls both neuronal maturation and developmental survival.Cell Death Differ. 2008; 15: 1408-1416
- Serine protease HtrA1 associates with microtubules and inhibits cell migration.Mol Cell Biol. 2009; 29: 4177-4187
- HtrA1 inhibits mineral deposition by osteoblasts: requirement for the protease and PDZ domains.J Biol Chem. 2008; 283: 5928-5938
- Distribution of the serine protease HtrA1 in normal human tissues.J Histochem Cytochem. 2003; 51: 1279-1284
- Pattern of expression of HtrA1 during mouse development.J Histochem Cytochem. 2004; 52: 1609-1617
- Expression of mouse HtrA1 serine protease in normal bone and cartilage and its upregulation in joint cartilage damaged by experimental arthritis.Bone. 2005; 37: 323-336
- Role of HTRA1, a serine protease, in the progression of articular cartilage degeneration.Histol Histopathol. 2010; 25: 599-608
- HtrA1 serine protease inhibits signaling mediated by Tgfbeta family proteins.Development. 2004; 131: 1041-1053
- Role of matrix Gla protein in parathyroid hormone inhibition of osteoblast mineralization.Cells Tissues Organs. 2005; 181: 166-175
- Matrix Gla protein inhibition of tooth mineralization.J Dent Res. 2008; 87: 839-844
- Quantum dots-based immunofluorescence technology for the quantitative determination of HER2 expression in breast cancer.Biomaterials. 2009; 30: 2912-2918
- The use of multispectral imaging to distinguish reactive urothelium from neoplastic urothelium.J Pathol Inform. 2010; 1: 23
- A comparative study of using a combination of calcium chloride and mineral trioxide aggregate as the pulp-capping agent on dogs’ teeth.J Endod. 2011; 37: 786-788
- Histomorphometric and micro–computed tomography analysis of pulpal response to three different pulp capping materials.J Endod. 2011; 37: 507-512
- A hybrid approach to direct pulp capping by using Emdogain with a capping material.J Endod. 2011; 37: 667-672
- Vital pulp therapy in vital permanent teeth with cariously exposed pulp:a systematic review.J Endod. 2011; 37: 581-587
- Direct contact with mineral trioxide aggregate activates and differentiates human dental pulp cells.J Endod. 2011; 37: 1691-1695
- Immunohistochemical analysis of nestin, osteopontin, and proliferating cells in the reparative process of exposed dental pulp capped with mineral trioxide aggregate.J Endod. 2008; 34: 970-974
- Immunohistochemical expression of fibronectin and tenascin in human tooth pulp capped with mineral trioxide aggregate and a novel endodontic cement.J Endod. 2011; 37: 1613-1618
- A novel role for Twist-1 in pulp homeostasis.J Dent Res. 2007; 86: 951-955
- PLAP-1/asporin, a novel negative regulator of periodontal ligament mineralization.J Biol Chem. 2007; 282: 23070-23080
- The role of human HtrA1 in arthritic disease.J Biol Chem. 2006; 281: 6124-6129
- Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein.Nature. 1997; 386: 78-81
- Quantum dots light up pathology.J Pathol. 2008; 216: 275-285
- Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice.Folia Histochem Cytobiol. 2009; 47: 349-354
- Towards noncontact skin melanoma selection by multispectral imaging analysis.J Biomed Opt. 2011; 16: 060502
- Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes.Rheumatology (Oxford). 2009; 48: 1447-1450
- Carboxyl-terminal proteolytic processing of matrix Gla protein.J Biol Chem. 1991; 266: 21145-21149
- Processing and transport of matrix gamma-carboxyglutamic acid protein and bone morphogenetic protein-2 in cultured human vascular smooth muscle cells: evidence for an uptake mechanism for serum fetuin.J Biol Chem. 2004; 279: 43052-43060
Article info
Footnotes
Xianyu Li and Mi Zhou contributed equally to this work.
Supported by grants from the National Natural Science Foundation of China (no. 81070824), the Program for New Century Excellent Talents from the Ministry of Education of China (no. NCET-080-0412), the Fundamental Research Funds for the Central Universities (no. 304275741), and the PhD Candidates Self-research (including 1+4) Program of Wuhan University in 2008 (no. 2008010130400067).
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
