Abstract
Introduction
The aim of this study was to evaluate, by checkerboard DNA-DNA hybridization, the
composition of the microbiota of primary endodontic infections in cases associated
with exposed (n = 30) and unexposed (n = 30) pulp space.
Methods
Samples were collected by means of a #15 H-type file and 2 sterile paper points from
60 single-rooted teeth with necrotic pulp and periapical lesions. The presence, levels,
and proportions of 40 bacterial species were determined by checkerboard DNA-DNA hybridization.
Results
The species found in higher counts (×105) in exposed pulp space cases were Eubacterium saburreum, Fusobacterium nucleatum ssp. vincentii, Tannerella forsythia, Enterococcus faecalis, Neisseria mucosa, Campylobacter gracilis, and Prevotella nigrescens, and in unexposed pulp space cases they were F. nucleatum ssp. vincentii, N. mucosa, E. faecalis, E. saburreum, C. gracilis, and Porphyromonas gingivalis. Counts of F. nucleatum ssp. vincentii, Campylobacter sputigena, Capnocytophaga showae, Treponema socrenskii, Porphyromonas
endodontalis, Eikenella corrodens, and Capnocytophaga ochracea were significantly higher in unexposed pulp space cases (P < .05).
Conclusions
The data of the present investigation suggested specific differences between the composition
of the microbiota in cases with exposed and unexposed pulp space and an association
between higher levels of some specific species and unexposed pulp space cases.
Key Words
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Article info
Publication history
Published online: May 07, 2012
Identification
Copyright
© 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
