Abstract
Introduction
CPoint is a polymeric endodontic point that takes advantage of water-induced, non-isotropic
radial expansion to adapt to canal irregularities. This study evaluated the effects
of CPoint on the viability and mineralization potential of odontoblast-like cells.
Methods
The biocompatibility of CPoint and commercially available gutta-percha points was
evaluated by using a rat odontoblast-like cell line (MDPC-23). Cell viability was
evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,
flow cytometry, and confocal laser scanning microscopy. The mineralization potential
of MDPC-23 cells, in the presence of the root-filling materials, was evaluated by
examining the changes in osteogenic gene marker expression (quantitative real-time
polymerase chain reaction), alkaline phosphatase activity, alizarin red S assay, and
transmission electron microscopy.
Results
CPoint showed higher initial cytotoxicity compared with gutta-percha and Teflon (P < .05), which became nonsignificant after 4 immersion cycles. Significant differences
were also found between eluents from CPoint and gutta-percha at 1:1 concentration
(P < .05) but not at 1:10 or 1:100 concentration. Both materials induced minimal apoptosis-induced
alteration in plasma membrane permeability, as evidenced by flow cytometry and confocal
laser scanning microscopy. Compared with the Teflon negative control, CPoint and gutta-percha
groups showed up-regulation of most osteogenic gene markers except for dentin sialophosphoprotein,
which was down-regulated. Alkaline phosphatase activity and alizarin red assay for
CPoint and gutta-percha were both significantly higher than for Teflon but not significantly
different from each other (P > .05). Transmission electron microscopy showed discrete nodular electron-dense mineralization
foci in all 3 groups.
Conclusions
The in vitro biocompatibility of CPoint is comparable to gutta-percha with minimal adverse effects
on osteogenesis after elution of potentially toxic components.
Key Words
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Article info
Publication history
Published online: April 05, 2013
Footnotes
Drs Eid and Nikonov contributed equally to this work.
Identification
Copyright
Published by Elsevier Inc.
