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Tissue Dissolution by a Novel Multisonic Ultracleaning System and Sodium Hypochlorite

Published:February 10, 2014DOI:https://doi.org/10.1016/j.joen.2013.12.029

      Abstract

      Introduction

      This study aimed to evaluate the effectiveness of a novel Multisonic Ultracleaning System (Sonendo Inc, Laguna Hills, CA) in tissue dissolution in comparison with conventional irrigation devices.

      Methods

      Pieces of bovine muscle tissue (68 ± 2 mg) were placed in 0.7-mL test tubes (height: 23.60 mm, inner diameter: 6.00 mm, outer diameter: 7.75 mm) and exposed to 5 minutes of irrigation by different devices. Endodontic devices included the Multisonic Ultracleaning System, the Piezon Master 700 (EMS, Dallas, TX) ultrasonic system with agitation, the EndoVac negative-pressure irrigation system (SybronEndo, Orange, CA), and a conventional positive-pressure 27-G irrigation needle at a flow rate of 10 mL/min. The systems were tested with 0.5%, 3%, and 6% sodium hypochlorite (NaOCl) at room temperature (21°C) as well as 40°C. Irrigation with sterile water was used as a control. The mass of tissue specimens was measured and recorded before and after the use of each device, and if the specimen was completely dissolved visually within 5 minutes, the dissolution time was recorded. The rate of tissue dissolution (%/s) was then calculated.

      Results

      The Multisonic Ultracleaning System had the fastest rate of tissue dissolution (P < .05), at 1.0% ± 0.1% per second using 0.5% NaOCl, 2.3% ± 0.9% per second using 3% NaOCl, and 2.9% ± 0.7% per second using 6% NaOCl. This tissue dissolution rate was more than 8 times greater than the second fastest device tested (P < .01), the Piezon Master 700 ultrasonic system, which resulted in a tissue dissolution rate of 0.328% ± 0.002% per second using 6% NaOCl at 40°C. For all irrigation devices tested, the rate of tissue dissolution increased with a higher concentration and temperature of the NaOCl solution.

      Conclusions

      The novel Multisonic Ultracleaning System achieved a significantly faster tissue dissolution rate when compared with the other systems examined in vitro.

      Key Words

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      References

        • Cunningham W.T.
        • Balekjian A.Y.
        Effect of temperature on collagen-dissolving ability of sodium hypochlorite endodontic irrigant.
        Oral Surg Oral Med Oral Pathol. 1980; 49: 175-177
        • Cunningham W.T.
        • Joseph S.W.
        Effect of temperature on the bactericidal action of sodium hypochlorite endodontic irrigant.
        Oral Surg Oral Med Oral Pathol. 1980; 50: 569-571
        • Sedgley C.M.
        • Nagel A.C.
        • Hall D.
        • et al.
        Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real-time imaging in vitro.
        Int Endod J. 2005; 38: 97-104
        • Gutarts R.
        • Nusstein J.
        • Reader A.
        • et al.
        In vivo debridement efficacy of ultrasonic irrigation following hand-rotary instrumentation in human mandibular molars.
        J Endod. 2005; 31: 166-170
        • van der Sluis L.W.
        • Gambarini G.
        • Wu M.K.
        • et al.
        The influence of volume, type of irrigant and flushing method on removing artificially placed dentine debris from the apical root canal during passive ultrasonic irrigation.
        Int Endod J. 2006; 39: 472-476
        • Zehnder M.
        Root canal irrigants.
        J Endod. 2006; 32: 389-398
        • Gu L.S.
        • Kim J.R.
        • Ling J.
        • et al.
        Review of contemporary irrigant agitation techniques and devices.
        J Endod. 2009; 35: 791-804
        • Haapasalo M.
        • Shen Y.
        • Qian W.
        • et al.
        Irrigation in endodontics.
        Dent Clin North Am. 2010; 54: 291-312
        • Rosenfeld E.F.
        • James G.A.
        • Burch B.S.
        Vital pulp tissue response to sodium hypochlorite.
        J Endod. 1978; 4: 140-146
        • Turkun M.
        • Cengiz T.
        The effects of sodium hypochlorite and calcium hydroxide on tissue dissolution and root canal cleanliness.
        Int Endod J. 1997; 30: 335-342
        • Moorer W.R.
        • Wesselink P.R.
        Factors promoting the tissue dissolving capability of sodium hypochlorite.
        Int Endod J. 1982; 15: 187-196
        • Stojicic S.
        • Zivkovic S.
        • Qian W.
        • et al.
        Tissue dissolution by sodium hypochlorite: effect of concentration, temperature, agitation, and surfactant.
        J Endod. 2010; 36: 1558-1562
        • Ahmad M.
        • Pitt Ford T.J.
        • Crum L.A.
        Ultrasonic debridement of root canals: acoustic streaming and its possible role.
        J Endod. 1987; 13: 490-499
        • Cameron J.A.
        The synergistic relationship between ultrasound and sodium hypochlorite: a scanning electron microscope evaluation.
        J Endod. 1987; 13: 541-545
        • Lumley P.J.
        • Walmsley A.D.
        • Laird W.R.
        Streaming patterns produced around endosonic files.
        Int Endod J. 1991; 24: 290-297
        • Macedo R.G.
        • Wesselink P.R.
        • Zaccheo F.
        • et al.
        Reaction rate of NaOCl in contact with bovine dentine: effect of activation, exposure time, concentration and pH.
        Int Endod J. 2010; 43: 1108-1115
        • Okino L.A.
        • Siqueira E.L.
        • Santos M.
        • et al.
        Dissolution of pulp tissue by aqueous solution of chlorhexidine digluconate and chlorhexidine digluconate gel.
        Int Endod J. 2004; 37: 38-41
        • Hasselgren G.
        • Olsson B.
        • Cvek M.
        Effects of calcium hydroxide and sodium hypochlorite on the dissolution of necrotic porcine muscle tissue.
        J Endod. 1988; 14: 125-127
        • Tinaz A.C.
        • Alacam T.
        • Uzun O.
        • et al.
        The effect of disruption of apical constriction on periapical extrusion.
        J Endod. 2005; 31: 533-535
        • Mitchell R.P.
        • Baumgartner J.C.
        • Sedgley C.M.
        Apical extrusion of sodium hypochlorite using different root canal irrigation systems.
        J Endod. 2011; 37: 1677-1681
      1. Budris A, Mayleben PA. Effects of entrained air, NPSH margin, and suction piping on cavitation in centrifugal pumps. Proceedings of the 15th International Pump Users Symposium. Houston: Turbomachinery Laboratory; 1998:99–108.

        • de Sermeno R.F.
        • da Silva L.A.
        • Herrera H.
        • et al.
        Tissue damage after sodium hypochlorite extrusion during root canal treatment.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108: e46-e49