Abstract
Introduction
Matrix metalloproteinase (MMP) inhibition may improve endodontic treatment prognosis.
The purpose of this study was to determine if zinc incorporation into experimental
resin cements containing bioactive fillers may modulate MMP-mediated collagen degradation
of dentin.
Methods
Human dentin samples untreated and demineralized using 10% phosphoric acid or 0.5 mol/L
EDTA were infiltrated with the following experimental resins: (1) unfilled resin,
(2) resin with Bioglass 45S5 particles (OSspray, London, UK), (3) resin with beta-tricalcium
silicate particles (βTCS), (4) resin with zinc-doped Bioglass 45S5, and (5) resin
with zinc-doped βTCS particles. The specimens were stored in artificial saliva (for
24 hours, 1 week, and 4 weeks) and submitted to radioimmunoassay to quantify C-terminal
telopeptide. Scanning electron microscopy analysis was also undertaken on dentin samples
after 4 weeks of storage.
Results
Collagen degradation was prominent both in phosphoric acid and EDTA-treated dentin.
Resin infiltration strongly reduced MMP activity in demineralized dentin. Resin containing
Bioglass 45S5 particles exerted higher and stable protection of collagen. The presence
of zinc in βTCS particles increases MMP inhibition. Different mineral precipitation
was attained in dentin infiltrated with the resin cements containing bioactive fillers.
Conclusions
MMP degradation of dentin collagen is strongly reduced after resin infiltration of
dentin. Zinc incorporation in βTCS particles exerted an additional protection against
MMP-mediated collagen degradation. However, it did not occur in resin containing Bioglass
45S5 particles, probably because of the formation of phosphate-zinc compounds.
Key Words
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Article info
Publication history
Published online: August 13, 2014
Identification
Copyright
© 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
