Highlights
- •Bioactive gutta-percha was more biocompatible than conventional gutta-percha at each experimental time interval
- •The better tissue reactions promoted by bioactive gutta-percha might be explained by the presence of bioactive particles
- •Bioactive particles allows the precipitation of calcium phosphate at the material's surface forming a mineralized layer
- •To the present date, no study assessed the biological properties of this self-adhesive gutta-percha based material
Abstract
Introduction
The purpose of this study was to evaluate the biocompatibility of a self-adhesive
gutta-percha material and compare it with that of conventional gutta-percha.
Methods
Standard quantities of bioactive gutta-percha and conventional gutta-percha were directly
inserted subcutaneously into the dorsal connective tissue of 30 BALB/c mice according
to ISO 10993-6. After 7, 21, and 63 days each, 10 animals were euthanized, and the
materials and surrounding tissue were removed. Tissue samples were subjected to histological
processing resulting in 5-μm-thick slices stained with hematoxylin-eosin and Gomori
trichrome stain. A grade ranging from I–IV was used to classify the inflammatory reaction.
The Mann-Whitney U test with Bonferroni correction was used to compare the grade of inflammation induced
by the materials at each time point. Qualitative evaluation of biocompatibility over
time was also performed.
Results
Bioactive gutta-percha was more biocompatible than conventional gutta-percha at each
time interval (P < .05). Tissue exposed to bioactive gutta-percha reached “no inflammation” (grade
I) at the 21-day interval, whereas it took 63 days for the conventional gutta-percha
to reach the “slight inflammation” level (grade II).
Conclusions
Bioactive gutta-percha presented good tissue reaction at all time points. It may serve
as an alternative to gutta-percha in terms of biocompatibility.
Key Words
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Article info
Publication history
Published online: September 01, 2014
Identification
Copyright
© 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
